Rose rosette emaravirus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2YBR2|F2YBR2_9VIRU Replicase OS=Rose rosette emaravirus OX=1980433 PE=4 SV=1
MM1 pKa = 6.58 EE2 pKa = 4.95 QKK4 pKa = 9.59 TFMKK8 pKa = 9.99 TYY10 pKa = 10.17 KK11 pKa = 10.74 ALVNNKK17 pKa = 9.41 FSDD20 pKa = 3.78 HH21 pKa = 5.71 FCEE24 pKa = 5.31 LEE26 pKa = 3.98 TAIFNSMIEE35 pKa = 3.85 ILDD38 pKa = 3.53 NDD40 pKa = 3.81 EE41 pKa = 4.85 EE42 pKa = 4.55 YY43 pKa = 11.23 SEE45 pKa = 5.53 VIVLHH50 pKa = 6.91 DD51 pKa = 4.94 AYY53 pKa = 11.08 LKK55 pKa = 10.33 NQLEE59 pKa = 4.28 FSEE62 pKa = 5.2 INYY65 pKa = 6.9 QTQQIEE71 pKa = 3.98 ISGMKK76 pKa = 8.93 IQKK79 pKa = 9.79 KK80 pKa = 7.38 SLRR83 pKa = 11.84 KK84 pKa = 9.01 FLRR87 pKa = 11.84 YY88 pKa = 9.28 LQITCSLFGLTSVLPEE104 pKa = 4.6 SIHH107 pKa = 7.18 HH108 pKa = 6.94 DD109 pKa = 3.06 FHH111 pKa = 9.46 IFGTLVRR118 pKa = 11.84 GEE120 pKa = 4.68 RR121 pKa = 11.84 IFPYY125 pKa = 10.24 IPDD128 pKa = 3.54 HH129 pKa = 5.81 SQYY132 pKa = 11.86 LLMSSNIVKK141 pKa = 10.6 AIVKK145 pKa = 9.29 QVVLGYY151 pKa = 10.54 DD152 pKa = 3.52 PEE154 pKa = 4.23 DD155 pKa = 4.0 TIIKK159 pKa = 9.9 AQSQQQEE166 pKa = 4.16 DD167 pKa = 4.11 VNYY170 pKa = 10.81 LEE172 pKa = 4.38 STVAFLFHH180 pKa = 5.63 VHH182 pKa = 7.02 AIAYY186 pKa = 5.79 TTGRR190 pKa = 11.84 LNNKK194 pKa = 9.54 LPLEE198 pKa = 4.05 THH200 pKa = 6.59 QINNTINNASASSVMVIDD218 pKa = 5.21 DD219 pKa = 4.14 KK220 pKa = 11.85 YY221 pKa = 10.19 DD222 pKa = 3.35 TKK224 pKa = 11.34 LEE226 pKa = 3.84 EE227 pKa = 4.32 TVYY230 pKa = 10.87 HH231 pKa = 5.59 NHH233 pKa = 6.37
Molecular weight: 27.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.313
IPC2_protein 5.308
IPC_protein 5.296
Toseland 5.626
ProMoST 5.474
Dawson 5.461
Bjellqvist 5.512
Wikipedia 5.423
Rodwell 5.423
Grimsley 5.753
Solomon 5.461
Lehninger 5.448
Nozaki 5.677
DTASelect 5.855
Thurlkill 5.779
EMBOSS 5.728
Sillero 5.779
Patrickios 3.668
IPC_peptide 5.474
IPC2_peptide 5.792
IPC2.peptide.svr19 5.752
Protein with the highest isoelectric point:
>tr|A0A0K0M9E3|A0A0K0M9E3_9VIRU p5 OS=Rose rosette emaravirus OX=1980433 GN=ORF5 PE=4 SV=1
MM1 pKa = 7.44 LVLHH5 pKa = 5.96 QSWLLMINMILSLKK19 pKa = 9.19 KK20 pKa = 10.38 LCIIIIKK27 pKa = 9.03 TKK29 pKa = 9.01 TLIRR33 pKa = 11.84 CNKK36 pKa = 8.58 YY37 pKa = 8.04 WYY39 pKa = 10.18 CSISLSLNYY48 pKa = 10.27 NSLQALVSFIMFYY61 pKa = 10.42 II62 pKa = 4.27
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.623
IPC2_protein 9.107
IPC_protein 9.063
Toseland 9.472
ProMoST 9.341
Dawson 9.78
Bjellqvist 9.604
Wikipedia 9.97
Rodwell 10.014
Grimsley 9.867
Solomon 9.867
Lehninger 9.838
Nozaki 9.721
DTASelect 9.531
Thurlkill 9.633
EMBOSS 9.911
Sillero 9.78
Patrickios 2.562
IPC_peptide 9.853
IPC2_peptide 8.653
IPC2.peptide.svr19 8.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4825
62
2276
603.1
69.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.104 ± 0.538
1.762 ± 0.401
6.321 ± 0.421
5.741 ± 0.502
4.58 ± 0.193
3.13 ± 0.238
2.591 ± 0.407
9.451 ± 0.541
8.601 ± 0.209
9.492 ± 0.315
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.57 ± 0.231
7.109 ± 0.308
3.358 ± 0.234
2.528 ± 0.378
3.212 ± 0.333
7.088 ± 0.216
6.052 ± 0.491
5.803 ± 0.915
0.56 ± 0.155
5.948 ± 0.665
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here