Dinghuibacter silviterrae
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5399 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R8DFI1|A0A4R8DFI1_9BACT Uncharacterized protein OS=Dinghuibacter silviterrae OX=1539049 GN=EDB95_3501 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.31 HH3 pKa = 6.62 LLTLLCLVILGGLSSFKK20 pKa = 10.82 AVADD24 pKa = 4.2 NITFTVSINSPYY36 pKa = 10.31 DD37 pKa = 3.0 KK38 pKa = 10.52 HH39 pKa = 8.59 GSVEE43 pKa = 4.49 LVNQDD48 pKa = 3.02 TFADD52 pKa = 3.47 NTYY55 pKa = 10.15 YY56 pKa = 10.56 FAEE59 pKa = 5.17 GSSGFQVTIPTGNYY73 pKa = 9.5 DD74 pKa = 3.78 VLVDD78 pKa = 4.2 VNNTANDD85 pKa = 3.82 GLFSVTFNGTTQSADD100 pKa = 2.82 WPGEE104 pKa = 4.08 FFCSWDD110 pKa = 3.28 NVQITDD116 pKa = 4.7 AQNTWLSIADD126 pKa = 3.54
Molecular weight: 13.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.503
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.859
Patrickios 0.477
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|A0A4R8DPJ3|A0A4R8DPJ3_9BACT Putative Zn-dependent peptidase OS=Dinghuibacter silviterrae OX=1539049 GN=EDB95_0665 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPHH8 pKa = 4.58 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 6.91 TVHH16 pKa = 6.14 GFRR19 pKa = 11.84 KK20 pKa = 10.11 RR21 pKa = 11.84 MQTANGRR28 pKa = 11.84 KK29 pKa = 8.71 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.54 GRR39 pKa = 11.84 KK40 pKa = 8.93 RR41 pKa = 11.84 LTVSDD46 pKa = 4.21 DD47 pKa = 3.62 SRR49 pKa = 11.84 LKK51 pKa = 10.83
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.252
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.974
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5399
0
5399
2034043
29
4174
376.7
41.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.673 ± 0.039
0.867 ± 0.012
5.492 ± 0.023
4.849 ± 0.039
4.575 ± 0.026
7.935 ± 0.034
2.179 ± 0.017
5.632 ± 0.026
4.701 ± 0.035
9.866 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.016
4.309 ± 0.037
4.773 ± 0.025
3.719 ± 0.022
5.207 ± 0.031
6.1 ± 0.034
6.364 ± 0.054
6.974 ± 0.028
1.46 ± 0.012
4.139 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here