Grapevine badnavirus 1

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4N979|A0A2H4N979_9VIRU Uncharacterized protein OS=Grapevine badnavirus 1 OX=2052838 PE=4 SV=1
MM1 pKa = 7.01EE2 pKa = 5.27HH3 pKa = 6.91AGEE6 pKa = 4.59GATATIPGNSHH17 pKa = 5.72GRR19 pKa = 11.84SKK21 pKa = 10.07TEE23 pKa = 3.68TQQGHH28 pKa = 7.89LITTQPNNWEE38 pKa = 3.87NGEE41 pKa = 4.12LFRR44 pKa = 11.84SYY46 pKa = 11.4SNTVQRR52 pKa = 11.84GHH54 pKa = 6.2EE55 pKa = 4.06PGGISLSEE63 pKa = 4.26FIKK66 pKa = 10.8LRR68 pKa = 11.84AQLKK72 pKa = 9.29EE73 pKa = 3.98IEE75 pKa = 4.42SLAITRR81 pKa = 11.84ALVCLKK87 pKa = 9.68EE88 pKa = 4.12LRR90 pKa = 11.84DD91 pKa = 3.31IHH93 pKa = 5.99TIKK96 pKa = 10.64LDD98 pKa = 3.49EE99 pKa = 4.49CRR101 pKa = 11.84KK102 pKa = 9.63SSSPGRR108 pKa = 11.84DD109 pKa = 2.84GNYY112 pKa = 9.64WSDD115 pKa = 3.28HH116 pKa = 6.2CPSCQHH122 pKa = 6.79HH123 pKa = 7.03DD124 pKa = 3.65RR125 pKa = 11.84NLEE128 pKa = 4.21EE129 pKa = 4.87ILSQLEE135 pKa = 3.8RR136 pKa = 11.84VAADD140 pKa = 3.38VQKK143 pKa = 11.15FSLL146 pKa = 3.98

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4N978|A0A2H4N978_9VIRU Reverse transcriptase OS=Grapevine badnavirus 1 OX=2052838 PE=4 SV=1
MM1 pKa = 7.58SNYY4 pKa = 9.9LYY6 pKa = 10.89SQGTVTYY13 pKa = 10.14KK14 pKa = 10.56EE15 pKa = 4.34AIKK18 pKa = 9.5ATEE21 pKa = 4.47SIEE24 pKa = 4.31SPALGFVKK32 pKa = 10.51PSDD35 pKa = 3.76YY36 pKa = 10.86KK37 pKa = 11.3GGISAPTAQIKK48 pKa = 9.67QNNTQLQILVGISEE62 pKa = 4.59SLRR65 pKa = 11.84DD66 pKa = 3.5IKK68 pKa = 11.27DD69 pKa = 3.34DD70 pKa = 3.8LKK72 pKa = 11.29VIRR75 pKa = 11.84EE76 pKa = 4.25SLRR79 pKa = 11.84QIQSKK84 pKa = 10.11EE85 pKa = 4.04GSSSSAALPEE95 pKa = 4.02EE96 pKa = 4.54LVEE99 pKa = 4.31KK100 pKa = 10.81LNNLSLGEE108 pKa = 4.24SKK110 pKa = 10.62PSRR113 pKa = 11.84EE114 pKa = 3.56KK115 pKa = 10.64RR116 pKa = 11.84GQLRR120 pKa = 11.84VFKK123 pKa = 10.65DD124 pKa = 3.2PLRR127 pKa = 11.84ILEE130 pKa = 4.11EE131 pKa = 3.97EE132 pKa = 4.21KK133 pKa = 11.2AKK135 pKa = 11.01LKK137 pKa = 10.83

Molecular weight: 15.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2129

137

1846

709.7

81.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.73 ± 0.067

1.644 ± 0.439

4.979 ± 0.528

8.783 ± 0.54

3.241 ± 0.712

6.059 ± 0.412

2.114 ± 0.921

6.576 ± 0.188

6.811 ± 1.059

8.032 ± 1.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 1.035

4.65 ± 0.264

4.556 ± 0.355

5.449 ± 0.113

6.435 ± 0.338

6.858 ± 2.008

4.838 ± 0.395

5.355 ± 0.912

1.456 ± 0.416

3.57 ± 0.737
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski