Staphylococcus phage StauST398-2
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9QYQ9|M9QYQ9_9CAUD Phage tail tape measure protein-like protein OS=Staphylococcus phage StauST398-2 OX=1277672 GN=StauST398-2_0050 PE=4 SV=1
MM1 pKa = 7.82 IYY3 pKa = 10.52 FEE5 pKa = 4.76 SPYY8 pKa = 10.47 SAEE11 pKa = 3.98 TVADD15 pKa = 4.3 FEE17 pKa = 4.64 LTATSTFWGLAGG29 pKa = 4.1
Molecular weight: 3.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.281
IPC2_protein 3.77
IPC_protein 3.172
Toseland 3.185
ProMoST 3.554
Dawson 3.274
Bjellqvist 3.503
Wikipedia 3.338
Rodwell 3.16
Grimsley 3.172
Solomon 2.943
Lehninger 2.893
Nozaki 3.554
DTASelect 3.376
Thurlkill 3.363
EMBOSS 3.338
Sillero 3.376
Patrickios 0.006
IPC_peptide 2.931
IPC2_peptide 3.274
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|M9QYP6|M9QYP6_9CAUD Terminase small subunit OS=Staphylococcus phage StauST398-2 OX=1277672 GN=StauST398-2_0035 PE=4 SV=1
MM1 pKa = 6.76 QHH3 pKa = 5.98 WKK5 pKa = 7.98 QTGLNLTARR14 pKa = 11.84 RR15 pKa = 11.84 EE16 pKa = 4.02 RR17 pKa = 11.84 GVVSFVASMLHH28 pKa = 6.02 HH29 pKa = 7.12
Molecular weight: 3.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.153
IPC2_protein 10.014
IPC_protein 11.55
Toseland 11.725
ProMoST 12.208
Dawson 11.74
Bjellqvist 11.711
Wikipedia 12.193
Rodwell 11.506
Grimsley 11.769
Solomon 12.208
Lehninger 12.12
Nozaki 11.725
DTASelect 11.711
Thurlkill 11.725
EMBOSS 12.223
Sillero 11.725
Patrickios 11.374
IPC_peptide 12.223
IPC2_peptide 11.199
IPC2.peptide.svr19 9.478
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
13880
29
2066
223.9
25.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.749 ± 0.597
0.497 ± 0.111
6.282 ± 0.308
7.68 ± 0.399
4.071 ± 0.235
6.059 ± 0.546
1.787 ± 0.135
6.988 ± 0.378
9.805 ± 0.53
8.386 ± 0.307
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.514 ± 0.115
6.318 ± 0.249
2.781 ± 0.256
3.61 ± 0.159
4.078 ± 0.189
6.463 ± 0.349
5.648 ± 0.263
5.692 ± 0.245
1.297 ± 0.144
4.294 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here