Pseudomonas rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089ZRC2|A0A089ZRC2_9PSED YccS/YhfK family integral membrane protein OS=Pseudomonas rhizosphaerae OX=216142 GN=LT40_17670 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.04KK3 pKa = 8.44WQCIVCGLIYY13 pKa = 10.62DD14 pKa = 4.59EE15 pKa = 4.86ADD17 pKa = 2.73GWPEE21 pKa = 3.85DD22 pKa = 4.35GIAAGTLWQDD32 pKa = 3.46VPEE35 pKa = 5.1DD36 pKa = 4.13WLCPDD41 pKa = 4.21CGVGKK46 pKa = 9.63MDD48 pKa = 4.86FEE50 pKa = 4.76MIEE53 pKa = 3.9IGG55 pKa = 4.22

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089YR61|A0A089YR61_9PSED UvrABC system protein B OS=Pseudomonas rhizosphaerae OX=216142 GN=uvrB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.28NGRR28 pKa = 11.84TVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3893

0

3893

1299839

38

6317

333.9

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.332 ± 0.051

0.96 ± 0.014

5.429 ± 0.029

5.49 ± 0.038

3.58 ± 0.027

8.023 ± 0.04

2.294 ± 0.024

4.781 ± 0.03

3.34 ± 0.035

11.597 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.02

2.931 ± 0.029

4.84 ± 0.025

4.648 ± 0.033

6.4 ± 0.044

5.697 ± 0.028

5.015 ± 0.047

7.412 ± 0.039

1.391 ± 0.017

2.471 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski