Rheinheimera sp. F8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera; unclassified Rheinheimera

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4W1V6|A0A0U4W1V6_9GAMM Dephospho-CoA kinase OS=Rheinheimera sp. F8 OX=1763998 GN=coaE PE=3 SV=1
MM1 pKa = 7.66SDD3 pKa = 2.81NRR5 pKa = 11.84LFLVYY10 pKa = 10.51DD11 pKa = 3.75PAFDD15 pKa = 5.14DD16 pKa = 4.97MDD18 pKa = 6.32AEE20 pKa = 4.68GCPAFGYY27 pKa = 10.63VLLFNEE33 pKa = 4.97ADD35 pKa = 3.39ADD37 pKa = 4.25LYY39 pKa = 11.35KK40 pKa = 10.89SGEE43 pKa = 4.25NPPFAAVSLLFTDD56 pKa = 4.61HH57 pKa = 7.23ADD59 pKa = 3.48EE60 pKa = 5.46SISGDD65 pKa = 3.64LLGWAQLDD73 pKa = 3.81APEE76 pKa = 4.92LQDD79 pKa = 4.29FPLGYY84 pKa = 10.46FFMLMEE90 pKa = 4.0QAAQVAINAYY100 pKa = 8.78RR101 pKa = 11.84QVGHH105 pKa = 6.26VPDD108 pKa = 4.34RR109 pKa = 11.84LIALNMPEE117 pKa = 5.16DD118 pKa = 4.4DD119 pKa = 5.66LIQFDD124 pKa = 4.04VQFANLQLEE133 pKa = 4.55DD134 pKa = 4.21KK135 pKa = 10.72DD136 pKa = 5.75AEE138 pKa = 4.37VQLAQQMMAGRR149 pKa = 11.84PYY151 pKa = 10.95LDD153 pKa = 3.2SS154 pKa = 4.12

Molecular weight: 17.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4VZS1|A0A0U4VZS1_9GAMM FAD-binding FR-type domain-containing protein OS=Rheinheimera sp. F8 OX=1763998 GN=ATY27_04855 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.26QKK14 pKa = 10.16RR15 pKa = 11.84NHH17 pKa = 5.58GFRR20 pKa = 11.84ARR22 pKa = 11.84MADD25 pKa = 3.21KK26 pKa = 10.82NGRR29 pKa = 11.84AVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.73GRR40 pKa = 11.84KK41 pKa = 8.88RR42 pKa = 11.84LTVV45 pKa = 3.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2872

0

2872

1005369

45

3531

350.1

38.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.622 ± 0.051

1.007 ± 0.014

5.263 ± 0.034

5.021 ± 0.046

3.934 ± 0.025

6.837 ± 0.047

2.204 ± 0.019

4.92 ± 0.035

4.131 ± 0.046

11.996 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.024

3.381 ± 0.035

4.493 ± 0.028

6.568 ± 0.064

5.092 ± 0.033

6.084 ± 0.037

5.119 ± 0.041

6.76 ± 0.032

1.43 ± 0.02

2.914 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski