Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946)
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9URS8|A0A1L9URS8_ASPBC Uncharacterized protein OS=Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946) OX=767769 GN=ASPBRDRAFT_193058 PE=4 SV=1
MM1 pKa = 7.18 VSSSVIVLTLWAALVSASPVADD23 pKa = 3.73 PLVTPAPKK31 pKa = 10.7 LEE33 pKa = 4.33 DD34 pKa = 3.46 LEE36 pKa = 4.92 KK37 pKa = 10.62 RR38 pKa = 11.84 ATSCTFSGSKK48 pKa = 9.97 GASSASKK55 pKa = 10.7 SKK57 pKa = 9.53 TSCSTIVLSDD67 pKa = 3.44 VAVPSGTTLDD77 pKa = 3.9 LTDD80 pKa = 5.4 LNDD83 pKa = 3.69 GTHH86 pKa = 6.48 VIFEE90 pKa = 4.49 GEE92 pKa = 4.06 TTFGYY97 pKa = 10.42 KK98 pKa = 9.1 EE99 pKa = 3.82 WSGPLVSVSGTDD111 pKa = 3.23 ITVTGADD118 pKa = 3.38 GAYY121 pKa = 10.71 LNGDD125 pKa = 3.92 GSRR128 pKa = 11.84 WWDD131 pKa = 3.38 DD132 pKa = 3.26 EE133 pKa = 4.13 GSNGGKK139 pKa = 8.24 TKK141 pKa = 10.59 PKK143 pKa = 10.12 FFYY146 pKa = 10.74 AHH148 pKa = 7.7 DD149 pKa = 4.24 LTSSTISGIYY159 pKa = 8.66 IQNSPVQVFSIDD171 pKa = 3.1 GSTDD175 pKa = 3.07 LTLEE179 pKa = 5.37 DD180 pKa = 3.42 ITIDD184 pKa = 3.96 NSDD187 pKa = 3.9 GDD189 pKa = 4.52 DD190 pKa = 3.38 EE191 pKa = 5.12 GAANTDD197 pKa = 3.17 GFDD200 pKa = 3.88 IGDD203 pKa = 3.68 STYY206 pKa = 9.73 ITITGANVYY215 pKa = 10.14 NQDD218 pKa = 3.1 DD219 pKa = 4.24 CVAVNSGEE227 pKa = 4.07 NIYY230 pKa = 10.7 FSGGVCSGGHH240 pKa = 5.7 GLSIGSVGGRR250 pKa = 11.84 SDD252 pKa = 3.45 NTVKK256 pKa = 10.81 NVTFYY261 pKa = 11.3 NSEE264 pKa = 3.84 IKK266 pKa = 10.65 NSQNGVRR273 pKa = 11.84 IKK275 pKa = 9.88 TIYY278 pKa = 10.53 GDD280 pKa = 3.51 TGSVSEE286 pKa = 4.22 VTYY289 pKa = 11.1 KK290 pKa = 10.76 EE291 pKa = 3.78 ITLSEE296 pKa = 3.79 ITKK299 pKa = 10.57 YY300 pKa = 10.95 GIIVQQNYY308 pKa = 10.46 DD309 pKa = 3.91 DD310 pKa = 4.27 TSEE313 pKa = 4.37 SPTDD317 pKa = 4.3 GITIEE322 pKa = 5.64 DD323 pKa = 3.85 FVLDD327 pKa = 3.86 NVQGSVEE334 pKa = 4.1 SSGTNIYY341 pKa = 9.42 IVCGSDD347 pKa = 4.0 SCTDD351 pKa = 3.47 WTWTDD356 pKa = 2.93 VDD358 pKa = 3.88 VTGGKK363 pKa = 8.27 TSSSCEE369 pKa = 3.93 NVPDD373 pKa = 6.1 DD374 pKa = 4.49 ISCC377 pKa = 3.93
Molecular weight: 39.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.986
Patrickios 1.329
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.882
Protein with the highest isoelectric point:
>tr|A0A1L9UCD6|A0A1L9UCD6_ASPBC Component of oligomeric Golgi complex 2 OS=Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946) OX=767769 GN=ASPBRDRAFT_57044 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.78 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.25 WRR45 pKa = 11.84 KK46 pKa = 9.64 SRR48 pKa = 11.84 LGVV51 pKa = 3.24
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12981
1
12982
6024818
49
7067
464.1
51.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.422 ± 0.018
1.345 ± 0.008
5.59 ± 0.013
6.04 ± 0.019
3.749 ± 0.013
6.782 ± 0.02
2.49 ± 0.009
4.994 ± 0.016
4.357 ± 0.017
9.205 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.008
3.55 ± 0.011
6.024 ± 0.02
4.017 ± 0.016
6.137 ± 0.018
8.316 ± 0.027
6.061 ± 0.017
6.262 ± 0.016
1.526 ± 0.008
2.951 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here