Companilactobacillus allii
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2425 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8Q5T7|A0A1P8Q5T7_9LACO LacI family transcriptional regulator OS=Companilactobacillus allii OX=1847728 GN=BTM29_12025 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.57 NLIKK6 pKa = 10.11 GTAVLGAIVVGAASTFLTVGAATTDD31 pKa = 3.45 AAEE34 pKa = 4.24 TSPAPTTTNSTATITQTGALNLDD57 pKa = 3.81 SAPSIDD63 pKa = 4.83 FGSQAYY69 pKa = 7.49 TVGEE73 pKa = 4.08 TKK75 pKa = 10.55 YY76 pKa = 10.26 ASSAIGSDD84 pKa = 3.29 LHH86 pKa = 5.45 VTNPGFASGWSVTVSATPFTDD107 pKa = 3.09 SATSNTLKK115 pKa = 10.13 GTTLSLDD122 pKa = 3.43 SSKK125 pKa = 10.9 AAPITADD132 pKa = 3.34 DD133 pKa = 3.97 SSNVSGLPSYY143 pKa = 9.61 VAPLTIGSSATEE155 pKa = 4.12 VEE157 pKa = 4.45 SAGAGDD163 pKa = 4.06 GVGAYY168 pKa = 7.38 STSYY172 pKa = 10.62 GAADD176 pKa = 3.41 ASLNVPAGNLPGTYY190 pKa = 10.09 SSTLTWTLNDD200 pKa = 3.52 APAA203 pKa = 4.9
Molecular weight: 19.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.961
Patrickios 1.914
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A1P8Q145|A0A1P8Q145_9LACO Uncharacterized protein OS=Companilactobacillus allii OX=1847728 GN=BTM29_02830 PE=4 SV=1
MM1 pKa = 6.05 TTKK4 pKa = 9.88 RR5 pKa = 11.84 TYY7 pKa = 9.64 QPKK10 pKa = 8.58 KK11 pKa = 7.47 RR12 pKa = 11.84 HH13 pKa = 5.71 RR14 pKa = 11.84 EE15 pKa = 3.79 RR16 pKa = 11.84 VHH18 pKa = 7.7 GFMKK22 pKa = 10.54 RR23 pKa = 11.84 MSTSNGRR30 pKa = 11.84 RR31 pKa = 11.84 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.38 KK39 pKa = 10.05 GRR41 pKa = 11.84 KK42 pKa = 8.66 VLSAA46 pKa = 4.05
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2425
0
2425
738784
34
2044
304.7
34.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.408 ± 0.053
0.481 ± 0.012
6.419 ± 0.054
5.59 ± 0.053
4.475 ± 0.045
6.52 ± 0.047
1.765 ± 0.02
8.284 ± 0.054
7.545 ± 0.051
9.168 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.78 ± 0.023
5.899 ± 0.047
3.296 ± 0.026
3.519 ± 0.031
3.389 ± 0.039
6.876 ± 0.046
5.993 ± 0.052
6.861 ± 0.042
0.939 ± 0.017
3.794 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here