Suffolk virus
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1G6U7|A0A0A1G6U7_9VIRU Large structural protein OS=Suffolk virus OX=1577137 PE=4 SV=1
MM1 pKa = 6.73 STSGRR6 pKa = 11.84 SLSSCMLWVILCVLGGGQQAEE27 pKa = 4.3 GLVGFDD33 pKa = 3.64 CTGQAVNVTAVSLSWIQKK51 pKa = 9.34 CPVAEE56 pKa = 4.55 GAQGTIVEE64 pKa = 5.42 FIQLVQEE71 pKa = 4.4 RR72 pKa = 11.84 NVEE75 pKa = 4.05 AVRR78 pKa = 11.84 VKK80 pKa = 10.36 ACLVEE85 pKa = 4.55 RR86 pKa = 11.84 SYY88 pKa = 11.76 LLQHH92 pKa = 6.89 CGMHH96 pKa = 5.76 SHH98 pKa = 7.01 SSMTFNGLVTSEE110 pKa = 3.9 ILRR113 pKa = 11.84 VPKK116 pKa = 9.66 EE117 pKa = 4.02 ACDD120 pKa = 3.63 ALHH123 pKa = 7.1 LYY125 pKa = 10.9 GSFHH129 pKa = 6.29 TSGGKK134 pKa = 9.64 VLTGFAANTTTVVPVVEE151 pKa = 4.66 MGVIDD156 pKa = 5.4 AGSATCKK163 pKa = 10.46 GATFTLNGATYY174 pKa = 9.54 TDD176 pKa = 3.55 VVMQSSYY183 pKa = 11.21 KK184 pKa = 8.77 VTLRR188 pKa = 11.84 TEE190 pKa = 4.39 RR191 pKa = 11.84 AQLDD195 pKa = 3.99 VATSTVRR202 pKa = 11.84 LSSGYY207 pKa = 8.12 SHH209 pKa = 7.98 PFQAHH214 pKa = 5.83 SGFDD218 pKa = 3.52 PEE220 pKa = 5.68 LGQTYY225 pKa = 9.03 WSSEE229 pKa = 3.91 GVGVKK234 pKa = 10.29 CSPTSYY240 pKa = 9.74 IVVYY244 pKa = 10.37 EE245 pKa = 4.18 GDD247 pKa = 2.99 ATIYY251 pKa = 10.51 KK252 pKa = 9.84 NRR254 pKa = 11.84 EE255 pKa = 3.75 GQRR258 pKa = 11.84 TLIVNTTTQVMAVGLRR274 pKa = 11.84 DD275 pKa = 3.72 EE276 pKa = 4.56 TTLCHH281 pKa = 6.72 QEE283 pKa = 3.89 ATEE286 pKa = 4.15 TDD288 pKa = 3.42 HH289 pKa = 7.34 PRR291 pKa = 11.84 LFMVRR296 pKa = 11.84 PLRR299 pKa = 11.84 AGGPRR304 pKa = 11.84 FYY306 pKa = 10.45 FQKK309 pKa = 11.09 GSMDD313 pKa = 3.7 PKK315 pKa = 10.69 EE316 pKa = 4.19 VDD318 pKa = 2.99 LFLYY322 pKa = 9.58 TNSKK326 pKa = 9.61 LVYY329 pKa = 9.88 VEE331 pKa = 3.64 QHH333 pKa = 6.51 LARR336 pKa = 11.84 EE337 pKa = 4.24 LTSVYY342 pKa = 11.06 LHH344 pKa = 6.3 FQKK347 pKa = 10.64 QMCDD351 pKa = 3.11 VNHH354 pKa = 7.18 RR355 pKa = 11.84 LLTHH359 pKa = 6.56 LTTLAMVAPEE369 pKa = 4.05 EE370 pKa = 4.23 FAWTYY375 pKa = 6.94 TQQPGITAVLRR386 pKa = 11.84 GEE388 pKa = 4.05 VVYY391 pKa = 9.37 MVKK394 pKa = 10.35 CAPVSVDD401 pKa = 3.12 YY402 pKa = 10.46 RR403 pKa = 11.84 ASSACFQEE411 pKa = 5.13 IPVTYY416 pKa = 10.51 NGTQVFLKK424 pKa = 9.5 PRR426 pKa = 11.84 SRR428 pKa = 11.84 IITTYY433 pKa = 9.14 GTEE436 pKa = 4.1 IDD438 pKa = 4.71 CSPLAPPLFFLNGNWVSFAPHH459 pKa = 6.56 PTHH462 pKa = 6.66 VLPPQVLNASFQAEE476 pKa = 4.09 WAYY479 pKa = 9.78 TSPPHH484 pKa = 6.94 LISAGLYY491 pKa = 9.28 SQSLLEE497 pKa = 4.3 KK498 pKa = 10.09 YY499 pKa = 9.49 QRR501 pKa = 11.84 QLMFPIEE508 pKa = 3.96 RR509 pKa = 11.84 TAIEE513 pKa = 4.16 TTMAASMAGLNVDD526 pKa = 3.81 HH527 pKa = 6.98 QGLDD531 pKa = 2.98 ASGLLRR537 pKa = 11.84 QSGLDD542 pKa = 3.39 KK543 pKa = 10.98 LQTSFMEE550 pKa = 5.79 RR551 pKa = 11.84 IYY553 pKa = 10.81 GWWWTFSVNLAGVMGVIFVITAIKK577 pKa = 10.47 AIGNVVLNATFLYY590 pKa = 9.11 KK591 pKa = 10.49 TFGCGFRR598 pKa = 11.84 MFAMFWGTLAKK609 pKa = 10.46 YY610 pKa = 10.5 LLIKK614 pKa = 10.77 VPTNPSRR621 pKa = 11.84 DD622 pKa = 3.78 DD623 pKa = 3.69 VEE625 pKa = 6.36 AGTPSPPTSSPTPCSSGACNEE646 pKa = 3.98 EE647 pKa = 3.77 ATILISPPSSHH658 pKa = 7.57 FYY660 pKa = 10.21 PSVSYY665 pKa = 10.57 RR666 pKa = 11.84 PPGATAPIII675 pKa = 3.95
Molecular weight: 73.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.958
IPC2_protein 6.097
IPC_protein 6.173
Toseland 6.415
ProMoST 6.59
Dawson 6.532
Bjellqvist 6.532
Wikipedia 6.547
Rodwell 6.532
Grimsley 6.517
Solomon 6.532
Lehninger 6.532
Nozaki 6.825
DTASelect 6.985
Thurlkill 7.015
EMBOSS 6.985
Sillero 6.927
Patrickios 3.312
IPC_peptide 6.547
IPC2_peptide 6.854
IPC2.peptide.svr19 6.781
Protein with the highest isoelectric point:
>tr|A0A0A1G690|A0A0A1G690_9VIRU Putative glycoprotein OS=Suffolk virus OX=1577137 PE=4 SV=1
MM1 pKa = 7.94 GDD3 pKa = 3.65 PVVQPVVVEE12 pKa = 4.52 AGARR16 pKa = 11.84 AQLQAAPPGFWNPRR30 pKa = 11.84 DD31 pKa = 3.47 TEE33 pKa = 4.19 YY34 pKa = 11.0 QRR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.15 AFGGIVATDD47 pKa = 3.38 QLTGANCDD55 pKa = 4.04 FLAGTSDD62 pKa = 3.58 DD63 pKa = 5.02 AIFPRR68 pKa = 11.84 ITIMARR74 pKa = 11.84 GEE76 pKa = 4.22 AYY78 pKa = 10.54 LKK80 pKa = 10.71 AITDD84 pKa = 3.62 IEE86 pKa = 4.45 KK87 pKa = 9.45 ATLTIAIANAIAPNMTALALPPAEE111 pKa = 3.9 ASARR115 pKa = 11.84 GRR117 pKa = 11.84 TQVVEE122 pKa = 4.85 FLRR125 pKa = 11.84 QEE127 pKa = 3.72 VGKK130 pKa = 9.86 NVKK133 pKa = 9.09 DD134 pKa = 3.51 LCVITPQLWASARR147 pKa = 11.84 AAEE150 pKa = 4.9 GPLHH154 pKa = 7.03 RR155 pKa = 11.84 IPWLCDD161 pKa = 3.72 FNGLPTLVRR170 pKa = 11.84 PADD173 pKa = 3.67 GTDD176 pKa = 3.24 TVGHH180 pKa = 6.1 YY181 pKa = 10.96 NSILAFLLTYY191 pKa = 6.63 PTPYY195 pKa = 9.57 ISNIGTRR202 pKa = 11.84 LYY204 pKa = 10.84 AILYY208 pKa = 9.81 VSLAKK213 pKa = 10.39 QGTISRR219 pKa = 11.84 GKK221 pKa = 10.24 LEE223 pKa = 5.8 KK224 pKa = 10.23 INQDD228 pKa = 3.36 LAVNMGFEE236 pKa = 4.55 VNMTEE241 pKa = 4.22 EE242 pKa = 4.74 VITYY246 pKa = 7.6 TYY248 pKa = 10.0 QQIGTKK254 pKa = 9.5 IPHH257 pKa = 6.32 NALEE261 pKa = 4.23 IMFNTMRR268 pKa = 11.84 DD269 pKa = 3.2 HH270 pKa = 5.94 MQNLSLRR277 pKa = 11.84 MQVTLQQAAGTGLTGLQIIKK297 pKa = 10.16 RR298 pKa = 11.84 AILEE302 pKa = 4.27 HH303 pKa = 6.98 PAFPWGKK310 pKa = 9.12 LAQMLPAEE318 pKa = 4.6 GPKK321 pKa = 10.4 VIAAFEE327 pKa = 4.14 AVGNDD332 pKa = 3.43 PFYY335 pKa = 11.54 GFLPDD340 pKa = 4.73 LGPAKK345 pKa = 9.21 STNYY349 pKa = 9.86 ARR351 pKa = 11.84 YY352 pKa = 8.73 VWVCQRR358 pKa = 11.84 LLRR361 pKa = 11.84 KK362 pKa = 9.81 FNAEE366 pKa = 3.92 DD367 pKa = 3.47 EE368 pKa = 4.5 RR369 pKa = 11.84 TLRR372 pKa = 11.84 NYY374 pKa = 10.44 KK375 pKa = 10.39 GGVRR379 pKa = 11.84 GIPNQPLFEE388 pKa = 4.33 EE389 pKa = 5.78 LIDD392 pKa = 4.2 SYY394 pKa = 11.72 SPPAPEE400 pKa = 4.38 AAVSADD406 pKa = 3.73 FVALGVNITALARR419 pKa = 11.84 SCRR422 pKa = 11.84 ALQSS426 pKa = 3.36
Molecular weight: 46.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.706
IPC2_protein 6.868
IPC_protein 7.015
Toseland 6.795
ProMoST 7.307
Dawson 7.614
Bjellqvist 7.629
Wikipedia 7.541
Rodwell 7.6
Grimsley 6.868
Solomon 7.702
Lehninger 7.717
Nozaki 7.878
DTASelect 7.746
Thurlkill 7.79
EMBOSS 7.878
Sillero 8.039
Patrickios 4.291
IPC_peptide 7.702
IPC2_peptide 7.205
IPC2.peptide.svr19 7.263
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3382
113
2168
845.5
94.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.658 ± 1.353
2.07 ± 0.356
4.879 ± 0.824
5.707 ± 0.441
3.578 ± 0.471
6.062 ± 0.668
2.543 ± 0.402
4.967 ± 0.522
4.021 ± 0.469
10.408 ± 1.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.602 ± 0.151
3.459 ± 0.474
5.795 ± 1.001
3.637 ± 0.582
6.091 ± 0.729
5.943 ± 0.93
7.126 ± 0.739
7.895 ± 0.584
1.626 ± 0.283
3.933 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here