Amino acid dipepetide frequency for Escherichia phage DTL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.812AlaAla: 9.812 ± 1.442
0.649AlaCys: 0.649 ± 0.269
4.217AlaAsp: 4.217 ± 0.61
5.352AlaGlu: 5.352 ± 0.714
3.163AlaPhe: 3.163 ± 0.469
6.406AlaGly: 6.406 ± 0.84
1.135AlaHis: 1.135 ± 0.34
6.812AlaIle: 6.812 ± 0.653
6.649AlaLys: 6.649 ± 1.182
7.217AlaLeu: 7.217 ± 1.102
2.352AlaMet: 2.352 ± 0.51
4.379AlaAsn: 4.379 ± 0.673
2.189AlaPro: 2.189 ± 0.406
3.325AlaGln: 3.325 ± 0.748
5.028AlaArg: 5.028 ± 0.728
5.028AlaSer: 5.028 ± 0.728
4.946AlaThr: 4.946 ± 0.733
5.19AlaVal: 5.19 ± 0.648
0.973AlaTrp: 0.973 ± 0.311
2.271AlaTyr: 2.271 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.487CysAla: 0.487 ± 0.197
0.243CysCys: 0.243 ± 0.148
0.811CysAsp: 0.811 ± 0.277
0.73CysGlu: 0.73 ± 0.273
0.405CysPhe: 0.405 ± 0.182
1.297CysGly: 1.297 ± 0.348
0.162CysHis: 0.162 ± 0.1
0.487CysIle: 0.487 ± 0.205
1.054CysLys: 1.054 ± 0.303
0.892CysLeu: 0.892 ± 0.251
0.162CysMet: 0.162 ± 0.112
0.73CysAsn: 0.73 ± 0.268
0.405CysPro: 0.405 ± 0.159
0.162CysGln: 0.162 ± 0.128
0.892CysArg: 0.892 ± 0.313
1.297CysSer: 1.297 ± 0.342
0.649CysThr: 0.649 ± 0.213
0.811CysVal: 0.811 ± 0.249
0.243CysTrp: 0.243 ± 0.122
0.243CysTyr: 0.243 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
5.028AspAla: 5.028 ± 0.891
0.405AspCys: 0.405 ± 0.188
3.325AspAsp: 3.325 ± 0.721
4.784AspGlu: 4.784 ± 0.536
1.946AspPhe: 1.946 ± 0.413
7.136AspGly: 7.136 ± 0.78
1.379AspHis: 1.379 ± 0.412
3.892AspIle: 3.892 ± 0.463
4.622AspLys: 4.622 ± 0.483
4.622AspLeu: 4.622 ± 0.617
1.054AspMet: 1.054 ± 0.266
3.163AspAsn: 3.163 ± 0.595
1.379AspPro: 1.379 ± 0.391
1.216AspGln: 1.216 ± 0.249
1.946AspArg: 1.946 ± 0.467
3.244AspSer: 3.244 ± 0.656
3.163AspThr: 3.163 ± 0.539
4.054AspVal: 4.054 ± 0.692
0.973AspTrp: 0.973 ± 0.263
2.676AspTyr: 2.676 ± 0.492
0.0AspXaa: 0.0 ± 0.0
Glu
6.082GluAla: 6.082 ± 0.683
0.892GluCys: 0.892 ± 0.321
3.0GluAsp: 3.0 ± 0.487
3.487GluGlu: 3.487 ± 0.744
3.568GluPhe: 3.568 ± 0.553
3.649GluGly: 3.649 ± 0.546
0.649GluHis: 0.649 ± 0.271
5.433GluIle: 5.433 ± 0.621
3.811GluLys: 3.811 ± 0.675
6.001GluLeu: 6.001 ± 0.727
2.757GluMet: 2.757 ± 0.667
3.649GluAsn: 3.649 ± 0.46
1.946GluPro: 1.946 ± 0.349
3.0GluGln: 3.0 ± 0.828
2.189GluArg: 2.189 ± 0.425
4.136GluSer: 4.136 ± 0.637
3.0GluThr: 3.0 ± 0.399
4.298GluVal: 4.298 ± 0.666
0.73GluTrp: 0.73 ± 0.294
2.595GluTyr: 2.595 ± 0.502
0.0GluXaa: 0.0 ± 0.0
Phe
2.433PheAla: 2.433 ± 0.493
0.973PheCys: 0.973 ± 0.316
2.838PheAsp: 2.838 ± 0.478
2.757PheGlu: 2.757 ± 0.562
0.892PhePhe: 0.892 ± 0.256
3.0PheGly: 3.0 ± 0.48
0.892PheHis: 0.892 ± 0.315
2.027PheIle: 2.027 ± 0.311
3.0PheLys: 3.0 ± 0.538
1.865PheLeu: 1.865 ± 0.458
1.054PheMet: 1.054 ± 0.251
2.514PheAsn: 2.514 ± 0.495
1.135PhePro: 1.135 ± 0.301
1.865PheGln: 1.865 ± 0.379
2.352PheArg: 2.352 ± 0.489
3.163PheSer: 3.163 ± 0.504
2.676PheThr: 2.676 ± 0.451
2.514PheVal: 2.514 ± 0.427
0.405PheTrp: 0.405 ± 0.159
0.811PheTyr: 0.811 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
4.703GlyAla: 4.703 ± 0.674
1.135GlyCys: 1.135 ± 0.303
3.811GlyAsp: 3.811 ± 0.626
4.379GlyGlu: 4.379 ± 0.653
2.919GlyPhe: 2.919 ± 0.365
5.433GlyGly: 5.433 ± 1.063
0.811GlyHis: 0.811 ± 0.312
5.352GlyIle: 5.352 ± 0.698
6.325GlyLys: 6.325 ± 0.876
6.487GlyLeu: 6.487 ± 0.563
1.865GlyMet: 1.865 ± 0.443
3.325GlyAsn: 3.325 ± 0.621
0.73GlyPro: 0.73 ± 0.242
2.676GlyGln: 2.676 ± 0.508
3.487GlyArg: 3.487 ± 0.45
5.595GlySer: 5.595 ± 0.62
3.811GlyThr: 3.811 ± 0.622
6.163GlyVal: 6.163 ± 0.784
1.135GlyTrp: 1.135 ± 0.317
4.46GlyTyr: 4.46 ± 0.722
0.0GlyXaa: 0.0 ± 0.0
His
0.892HisAla: 0.892 ± 0.282
0.405HisCys: 0.405 ± 0.176
0.973HisAsp: 0.973 ± 0.34
0.73HisGlu: 0.73 ± 0.248
0.568HisPhe: 0.568 ± 0.213
1.622HisGly: 1.622 ± 0.417
0.243HisHis: 0.243 ± 0.13
1.865HisIle: 1.865 ± 0.487
1.622HisLys: 1.622 ± 0.398
1.379HisLeu: 1.379 ± 0.345
0.405HisMet: 0.405 ± 0.216
0.568HisAsn: 0.568 ± 0.229
0.162HisPro: 0.162 ± 0.117
0.487HisGln: 0.487 ± 0.237
0.811HisArg: 0.811 ± 0.254
0.892HisSer: 0.892 ± 0.262
0.811HisThr: 0.811 ± 0.207
0.649HisVal: 0.649 ± 0.213
0.162HisTrp: 0.162 ± 0.117
0.405HisTyr: 0.405 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
6.163IleAla: 6.163 ± 0.992
0.487IleCys: 0.487 ± 0.199
6.082IleAsp: 6.082 ± 0.701
3.973IleGlu: 3.973 ± 0.474
1.703IlePhe: 1.703 ± 0.287
4.46IleGly: 4.46 ± 0.615
0.973IleHis: 0.973 ± 0.31
3.325IleIle: 3.325 ± 0.6
5.271IleLys: 5.271 ± 0.736
3.649IleLeu: 3.649 ± 0.64
1.541IleMet: 1.541 ± 0.44
3.73IleAsn: 3.73 ± 0.553
3.244IlePro: 3.244 ± 0.564
2.757IleGln: 2.757 ± 0.54
3.081IleArg: 3.081 ± 0.458
5.352IleSer: 5.352 ± 0.569
4.946IleThr: 4.946 ± 0.639
3.487IleVal: 3.487 ± 0.47
0.73IleTrp: 0.73 ± 0.22
2.676IleTyr: 2.676 ± 0.662
0.0IleXaa: 0.0 ± 0.0
Lys
6.893LysAla: 6.893 ± 0.997
0.568LysCys: 0.568 ± 0.244
4.217LysAsp: 4.217 ± 0.622
4.865LysGlu: 4.865 ± 0.827
3.325LysPhe: 3.325 ± 0.493
3.244LysGly: 3.244 ± 0.468
1.216LysHis: 1.216 ± 0.373
4.054LysIle: 4.054 ± 0.423
4.379LysLys: 4.379 ± 0.687
5.838LysLeu: 5.838 ± 0.848
2.919LysMet: 2.919 ± 0.527
3.0LysAsn: 3.0 ± 0.513
1.703LysPro: 1.703 ± 0.405
2.838LysGln: 2.838 ± 0.433
3.406LysArg: 3.406 ± 0.798
4.136LysSer: 4.136 ± 0.64
3.406LysThr: 3.406 ± 0.437
5.352LysVal: 5.352 ± 0.765
0.811LysTrp: 0.811 ± 0.295
3.0LysTyr: 3.0 ± 0.454
0.0LysXaa: 0.0 ± 0.0
Leu
6.568LeuAla: 6.568 ± 0.91
1.297LeuCys: 1.297 ± 0.258
4.622LeuAsp: 4.622 ± 0.649
4.054LeuGlu: 4.054 ± 0.65
2.352LeuPhe: 2.352 ± 0.392
5.109LeuGly: 5.109 ± 0.841
1.054LeuHis: 1.054 ± 0.355
5.433LeuIle: 5.433 ± 0.66
3.892LeuLys: 3.892 ± 0.516
4.136LeuLeu: 4.136 ± 0.514
1.46LeuMet: 1.46 ± 0.342
2.919LeuAsn: 2.919 ± 0.449
2.676LeuPro: 2.676 ± 0.427
2.433LeuGln: 2.433 ± 0.828
4.054LeuArg: 4.054 ± 0.629
6.649LeuSer: 6.649 ± 0.748
4.136LeuThr: 4.136 ± 0.65
5.514LeuVal: 5.514 ± 0.448
0.405LeuTrp: 0.405 ± 0.194
2.595LeuTyr: 2.595 ± 0.502
0.0LeuXaa: 0.0 ± 0.0
Met
3.081MetAla: 3.081 ± 0.465
0.162MetCys: 0.162 ± 0.119
0.811MetAsp: 0.811 ± 0.244
1.703MetGlu: 1.703 ± 0.459
1.216MetPhe: 1.216 ± 0.273
0.811MetGly: 0.811 ± 0.237
0.324MetHis: 0.324 ± 0.142
1.622MetIle: 1.622 ± 0.321
1.379MetLys: 1.379 ± 0.301
1.703MetLeu: 1.703 ± 0.409
0.811MetMet: 0.811 ± 0.356
1.622MetAsn: 1.622 ± 0.42
0.568MetPro: 0.568 ± 0.211
1.216MetGln: 1.216 ± 0.291
1.46MetArg: 1.46 ± 0.415
1.622MetSer: 1.622 ± 0.312
2.189MetThr: 2.189 ± 0.412
2.027MetVal: 2.027 ± 0.452
0.162MetTrp: 0.162 ± 0.107
0.73MetTyr: 0.73 ± 0.299
0.0MetXaa: 0.0 ± 0.0
Asn
4.379AsnAla: 4.379 ± 0.569
0.649AsnCys: 0.649 ± 0.208
2.271AsnAsp: 2.271 ± 0.362
4.379AsnGlu: 4.379 ± 0.663
1.784AsnPhe: 1.784 ± 0.395
5.757AsnGly: 5.757 ± 1.199
1.297AsnHis: 1.297 ± 0.388
2.271AsnIle: 2.271 ± 0.343
2.514AsnLys: 2.514 ± 0.415
3.487AsnLeu: 3.487 ± 0.664
0.973AsnMet: 0.973 ± 0.225
2.838AsnAsn: 2.838 ± 0.539
2.108AsnPro: 2.108 ± 0.413
2.433AsnGln: 2.433 ± 0.495
2.189AsnArg: 2.189 ± 0.445
3.973AsnSer: 3.973 ± 0.615
2.757AsnThr: 2.757 ± 0.466
3.73AsnVal: 3.73 ± 0.551
1.216AsnTrp: 1.216 ± 0.248
2.189AsnTyr: 2.189 ± 0.453
0.0AsnXaa: 0.0 ± 0.0
Pro
2.595ProAla: 2.595 ± 0.351
0.487ProCys: 0.487 ± 0.224
1.865ProAsp: 1.865 ± 0.511
3.244ProGlu: 3.244 ± 0.45
1.541ProPhe: 1.541 ± 0.344
1.541ProGly: 1.541 ± 0.296
0.487ProHis: 0.487 ± 0.226
2.433ProIle: 2.433 ± 0.346
1.135ProLys: 1.135 ± 0.307
1.703ProLeu: 1.703 ± 0.419
0.568ProMet: 0.568 ± 0.236
1.46ProAsn: 1.46 ± 0.376
0.568ProPro: 0.568 ± 0.179
1.135ProGln: 1.135 ± 0.352
1.379ProArg: 1.379 ± 0.338
1.135ProSer: 1.135 ± 0.308
1.379ProThr: 1.379 ± 0.305
3.0ProVal: 3.0 ± 0.619
0.487ProTrp: 0.487 ± 0.22
1.379ProTyr: 1.379 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
4.298GlnAla: 4.298 ± 1.059
0.324GlnCys: 0.324 ± 0.145
1.784GlnAsp: 1.784 ± 0.393
2.919GlnGlu: 2.919 ± 0.583
1.622GlnPhe: 1.622 ± 0.339
2.352GlnGly: 2.352 ± 0.438
0.487GlnHis: 0.487 ± 0.225
3.406GlnIle: 3.406 ± 0.633
2.189GlnLys: 2.189 ± 0.441
3.163GlnLeu: 3.163 ± 0.687
0.649GlnMet: 0.649 ± 0.221
2.027GlnAsn: 2.027 ± 0.467
0.973GlnPro: 0.973 ± 0.28
3.081GlnGln: 3.081 ± 1.311
1.784GlnArg: 1.784 ± 0.357
3.0GlnSer: 3.0 ± 0.576
1.297GlnThr: 1.297 ± 0.313
2.595GlnVal: 2.595 ± 0.388
0.405GlnTrp: 0.405 ± 0.188
1.054GlnTyr: 1.054 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
3.568ArgAla: 3.568 ± 0.49
0.811ArgCys: 0.811 ± 0.355
2.189ArgAsp: 2.189 ± 0.533
3.406ArgGlu: 3.406 ± 0.631
2.189ArgPhe: 2.189 ± 0.358
2.352ArgGly: 2.352 ± 0.355
0.568ArgHis: 0.568 ± 0.236
3.892ArgIle: 3.892 ± 0.6
4.541ArgLys: 4.541 ± 0.546
3.73ArgLeu: 3.73 ± 0.556
1.379ArgMet: 1.379 ± 0.382
2.189ArgAsn: 2.189 ± 0.473
1.703ArgPro: 1.703 ± 0.415
1.622ArgGln: 1.622 ± 0.382
2.919ArgArg: 2.919 ± 0.48
3.0ArgSer: 3.0 ± 0.484
2.189ArgThr: 2.189 ± 0.468
3.811ArgVal: 3.811 ± 0.571
0.568ArgTrp: 0.568 ± 0.198
2.189ArgTyr: 2.189 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
5.352SerAla: 5.352 ± 0.639
0.324SerCys: 0.324 ± 0.158
5.271SerAsp: 5.271 ± 0.618
4.865SerGlu: 4.865 ± 0.703
2.838SerPhe: 2.838 ± 0.416
6.325SerGly: 6.325 ± 0.637
1.297SerHis: 1.297 ± 0.29
4.541SerIle: 4.541 ± 0.505
5.109SerLys: 5.109 ± 0.755
4.946SerLeu: 4.946 ± 0.613
1.622SerMet: 1.622 ± 0.327
3.406SerAsn: 3.406 ± 0.715
1.946SerPro: 1.946 ± 0.414
2.676SerGln: 2.676 ± 0.503
2.919SerArg: 2.919 ± 0.476
3.649SerSer: 3.649 ± 0.669
3.487SerThr: 3.487 ± 0.749
4.946SerVal: 4.946 ± 0.488
0.649SerTrp: 0.649 ± 0.26
1.622SerTyr: 1.622 ± 0.339
0.0SerXaa: 0.0 ± 0.0
Thr
5.19ThrAla: 5.19 ± 0.749
0.487ThrCys: 0.487 ± 0.181
3.0ThrAsp: 3.0 ± 0.533
2.189ThrGlu: 2.189 ± 0.39
2.271ThrPhe: 2.271 ± 0.405
6.163ThrGly: 6.163 ± 0.817
0.892ThrHis: 0.892 ± 0.302
3.487ThrIle: 3.487 ± 0.512
3.0ThrLys: 3.0 ± 0.525
2.838ThrLeu: 2.838 ± 0.449
1.054ThrMet: 1.054 ± 0.278
3.649ThrAsn: 3.649 ± 0.619
2.352ThrPro: 2.352 ± 0.404
2.595ThrGln: 2.595 ± 0.436
1.946ThrArg: 1.946 ± 0.335
3.487ThrSer: 3.487 ± 0.474
2.838ThrThr: 2.838 ± 0.517
3.649ThrVal: 3.649 ± 0.515
0.568ThrTrp: 0.568 ± 0.187
2.433ThrTyr: 2.433 ± 0.497
0.0ThrXaa: 0.0 ± 0.0
Val
5.757ValAla: 5.757 ± 0.828
1.135ValCys: 1.135 ± 0.366
4.946ValAsp: 4.946 ± 0.479
3.568ValGlu: 3.568 ± 0.63
2.838ValPhe: 2.838 ± 0.467
4.946ValGly: 4.946 ± 0.509
0.892ValHis: 0.892 ± 0.244
4.136ValIle: 4.136 ± 0.614
5.19ValLys: 5.19 ± 0.578
4.217ValLeu: 4.217 ± 0.553
1.541ValMet: 1.541 ± 0.344
5.271ValAsn: 5.271 ± 0.58
2.108ValPro: 2.108 ± 0.431
2.027ValGln: 2.027 ± 0.603
4.379ValArg: 4.379 ± 0.731
4.865ValSer: 4.865 ± 0.651
3.73ValThr: 3.73 ± 0.589
5.514ValVal: 5.514 ± 0.829
1.054ValTrp: 1.054 ± 0.305
2.352ValTyr: 2.352 ± 0.496
0.0ValXaa: 0.0 ± 0.0
Trp
0.487TrpAla: 0.487 ± 0.194
0.162TrpCys: 0.162 ± 0.132
1.216TrpAsp: 1.216 ± 0.276
0.73TrpGlu: 0.73 ± 0.265
0.568TrpPhe: 0.568 ± 0.22
0.811TrpGly: 0.811 ± 0.229
0.324TrpHis: 0.324 ± 0.145
0.811TrpIle: 0.811 ± 0.257
1.379TrpLys: 1.379 ± 0.315
1.054TrpLeu: 1.054 ± 0.24
0.0TrpMet: 0.0 ± 0.0
0.811TrpAsn: 0.811 ± 0.24
0.324TrpPro: 0.324 ± 0.142
0.487TrpGln: 0.487 ± 0.193
0.892TrpArg: 0.892 ± 0.255
0.892TrpSer: 0.892 ± 0.408
0.487TrpThr: 0.487 ± 0.222
0.649TrpVal: 0.649 ± 0.21
0.081TrpTrp: 0.081 ± 0.074
0.324TrpTyr: 0.324 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.325TyrAla: 3.325 ± 0.575
0.568TyrCys: 0.568 ± 0.186
2.757TyrAsp: 2.757 ± 0.483
2.757TyrGlu: 2.757 ± 0.535
1.46TyrPhe: 1.46 ± 0.379
2.189TyrGly: 2.189 ± 0.402
0.568TyrHis: 0.568 ± 0.217
2.352TyrIle: 2.352 ± 0.448
2.189TyrLys: 2.189 ± 0.515
2.433TyrLeu: 2.433 ± 0.421
0.973TyrMet: 0.973 ± 0.259
1.946TyrAsn: 1.946 ± 0.425
1.297TyrPro: 1.297 ± 0.334
1.379TyrGln: 1.379 ± 0.364
1.703TyrArg: 1.703 ± 0.319
2.676TyrSer: 2.676 ± 0.497
2.189TyrThr: 2.189 ± 0.386
2.514TyrVal: 2.514 ± 0.419
0.73TyrTrp: 0.73 ± 0.241
1.297TyrTyr: 1.297 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (12333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski