Rhizobium tumorigenes
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5385 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W4BV51|A0A2W4BV51_9RHIZ Uncharacterized protein OS=Rhizobium tumorigenes OX=2041385 GN=CPJ17_02720 PE=4 SV=1
MM1 pKa = 7.86 SDD3 pKa = 3.76 LSLAQNHH10 pKa = 6.17 AFDD13 pKa = 4.45 LARR16 pKa = 11.84 TLMVPVTLFEE26 pKa = 5.21 IDD28 pKa = 3.61 GEE30 pKa = 4.23 IGVMISAEE38 pKa = 3.99 YY39 pKa = 10.81 DD40 pKa = 3.26 GDD42 pKa = 3.41 EE43 pKa = 4.18 DD44 pKa = 5.87 AIINIYY50 pKa = 10.9 DD51 pKa = 3.6 PFAYY55 pKa = 10.1 GSAHH59 pKa = 6.27
Molecular weight: 6.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.732
IPC_protein 3.63
Toseland 3.439
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.363
Solomon 3.605
Lehninger 3.567
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.77
Patrickios 0.604
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A2W4BVU9|A0A2W4BVU9_9RHIZ GntR family transcriptional regulator OS=Rhizobium tumorigenes OX=2041385 GN=CPJ17_07985 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.22 GGRR28 pKa = 11.84 KK29 pKa = 9.48 VIIARR34 pKa = 11.84 RR35 pKa = 11.84 GRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.08 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5385
0
5385
1661158
26
2833
308.5
33.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.724 ± 0.04
0.807 ± 0.01
5.851 ± 0.023
5.411 ± 0.034
3.888 ± 0.022
8.248 ± 0.033
2.037 ± 0.015
5.774 ± 0.026
3.699 ± 0.026
9.939 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.631 ± 0.014
2.875 ± 0.019
4.853 ± 0.029
3.184 ± 0.018
6.543 ± 0.033
6.101 ± 0.033
5.501 ± 0.024
7.373 ± 0.027
1.266 ± 0.013
2.295 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here