Pseudomonas sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519DSZ8|A0A519DSZ8_PSESP Sodium:proton antiporter OS=Pseudomonas sp. OX=306 GN=EOP12_03145 PE=4 SV=1
MM1 pKa = 7.3SLVDD5 pKa = 3.73TYY7 pKa = 11.61LAGLRR12 pKa = 11.84QSLVAEE18 pKa = 4.73DD19 pKa = 4.11LAQLKK24 pKa = 9.18HH25 pKa = 5.76AHH27 pKa = 6.18GASAADD33 pKa = 3.71LQRR36 pKa = 11.84LIEE39 pKa = 4.76RR40 pKa = 11.84YY41 pKa = 8.43PLCPASLLQLLGQIDD56 pKa = 3.85GTHH59 pKa = 5.67YY60 pKa = 10.45RR61 pKa = 11.84DD62 pKa = 3.74YY63 pKa = 11.32PNGEE67 pKa = 4.17VMVLMLGSDD76 pKa = 3.93LPGYY80 pKa = 9.48PYY82 pKa = 10.82YY83 pKa = 10.62LSSVADD89 pKa = 3.64ILTEE93 pKa = 4.14ADD95 pKa = 3.7GLDD98 pKa = 3.46TEE100 pKa = 5.52SIVEE104 pKa = 4.28LYY106 pKa = 10.54EE107 pKa = 3.87GWFEE111 pKa = 5.56KK112 pKa = 10.79GWLDD116 pKa = 4.87QEE118 pKa = 4.06MDD120 pKa = 4.68LIGEE124 pKa = 4.87GIDD127 pKa = 3.29PTVGMNEE134 pKa = 4.08LLCFSHH140 pKa = 7.41CINNGGTSKK149 pKa = 11.02LYY151 pKa = 10.17IDD153 pKa = 5.47FNPAPAGTVGQVVRR167 pKa = 11.84FLHH170 pKa = 7.28DD171 pKa = 4.04PDD173 pKa = 3.86NYY175 pKa = 10.73EE176 pKa = 4.08VIAPSFDD183 pKa = 4.03HH184 pKa = 6.81YY185 pKa = 10.95LQQLIDD191 pKa = 4.19DD192 pKa = 5.24DD193 pKa = 4.51YY194 pKa = 12.01AFIRR198 pKa = 11.84PEE200 pKa = 3.72PP201 pKa = 3.74

Molecular weight: 22.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G2NWK9|A0A2G2NWK9_PSESP 50S ribosomal protein L30 OS=Pseudomonas sp. OX=306 GN=rpmD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1027

0

1027

256579

23

2527

249.8

27.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.945 ± 0.1

0.922 ± 0.022

5.403 ± 0.064

5.743 ± 0.073

3.612 ± 0.05

7.528 ± 0.089

2.219 ± 0.042

5.2 ± 0.061

4.178 ± 0.071

10.338 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.037

3.248 ± 0.051

4.486 ± 0.059

4.051 ± 0.058

6.561 ± 0.076

6.5 ± 0.071

5.427 ± 0.06

7.399 ± 0.07

1.346 ± 0.031

2.447 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski