Amino acid dipepetide frequency for Apibacter muscae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.823AlaAla: 2.823 ± 0.078
0.553AlaCys: 0.553 ± 0.035
2.506AlaAsp: 2.506 ± 0.066
3.429AlaGlu: 3.429 ± 0.081
2.479AlaPhe: 2.479 ± 0.058
3.469AlaGly: 3.469 ± 0.086
0.906AlaHis: 0.906 ± 0.032
4.846AlaIle: 4.846 ± 0.1
4.281AlaLys: 4.281 ± 0.084
5.366AlaLeu: 5.366 ± 0.09
1.204AlaMet: 1.204 ± 0.041
2.759AlaAsn: 2.759 ± 0.069
1.515AlaPro: 1.515 ± 0.048
2.035AlaGln: 2.035 ± 0.06
1.723AlaArg: 1.723 ± 0.054
3.394AlaSer: 3.394 ± 0.08
2.837AlaThr: 2.837 ± 0.065
2.974AlaVal: 2.974 ± 0.074
0.477AlaTrp: 0.477 ± 0.026
2.272AlaTyr: 2.272 ± 0.063
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.028
0.088CysCys: 0.088 ± 0.012
0.378CysAsp: 0.378 ± 0.028
0.446CysGlu: 0.446 ± 0.027
0.479CysPhe: 0.479 ± 0.028
0.602CysGly: 0.602 ± 0.034
0.143CysHis: 0.143 ± 0.018
0.801CysIle: 0.801 ± 0.035
0.682CysLys: 0.682 ± 0.032
0.871CysLeu: 0.871 ± 0.038
0.163CysMet: 0.163 ± 0.015
0.514CysAsn: 0.514 ± 0.026
0.379CysPro: 0.379 ± 0.028
0.21CysGln: 0.21 ± 0.017
0.232CysArg: 0.232 ± 0.018
0.628CysSer: 0.628 ± 0.032
0.424CysThr: 0.424 ± 0.025
0.402CysVal: 0.402 ± 0.027
0.072CysTrp: 0.072 ± 0.011
0.346CysTyr: 0.346 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
2.534AspAla: 2.534 ± 0.075
0.389AspCys: 0.389 ± 0.022
2.089AspAsp: 2.089 ± 0.055
3.525AspGlu: 3.525 ± 0.067
3.131AspPhe: 3.131 ± 0.074
2.859AspGly: 2.859 ± 0.075
0.871AspHis: 0.871 ± 0.034
4.677AspIle: 4.677 ± 0.079
4.381AspLys: 4.381 ± 0.086
5.395AspLeu: 5.395 ± 0.093
0.86AspMet: 0.86 ± 0.029
2.802AspAsn: 2.802 ± 0.057
1.683AspPro: 1.683 ± 0.052
1.702AspGln: 1.702 ± 0.049
1.764AspArg: 1.764 ± 0.047
3.276AspSer: 3.276 ± 0.064
2.288AspThr: 2.288 ± 0.063
2.666AspVal: 2.666 ± 0.065
0.632AspTrp: 0.632 ± 0.035
2.688AspTyr: 2.688 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
3.67GluAla: 3.67 ± 0.084
0.434GluCys: 0.434 ± 0.026
3.558GluAsp: 3.558 ± 0.079
5.594GluGlu: 5.594 ± 0.113
3.148GluPhe: 3.148 ± 0.071
3.662GluGly: 3.662 ± 0.081
1.009GluHis: 1.009 ± 0.041
6.802GluIle: 6.802 ± 0.108
7.138GluLys: 7.138 ± 0.126
6.304GluLeu: 6.304 ± 0.115
1.415GluMet: 1.415 ± 0.05
5.388GluAsn: 5.388 ± 0.093
1.533GluPro: 1.533 ± 0.047
2.276GluGln: 2.276 ± 0.059
2.204GluArg: 2.204 ± 0.062
3.506GluSer: 3.506 ± 0.071
3.077GluThr: 3.077 ± 0.059
3.994GluVal: 3.994 ± 0.084
0.652GluTrp: 0.652 ± 0.032
2.967GluTyr: 2.967 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
2.288PheAla: 2.288 ± 0.056
0.493PheCys: 0.493 ± 0.028
2.577PheAsp: 2.577 ± 0.063
2.891PheGlu: 2.891 ± 0.057
3.021PhePhe: 3.021 ± 0.088
3.124PheGly: 3.124 ± 0.072
0.863PheHis: 0.863 ± 0.039
5.087PheIle: 5.087 ± 0.101
4.0PheLys: 4.0 ± 0.077
5.258PheLeu: 5.258 ± 0.105
1.103PheMet: 1.103 ± 0.037
3.48PheAsn: 3.48 ± 0.068
1.716PhePro: 1.716 ± 0.05
1.6PheGln: 1.6 ± 0.049
1.445PheArg: 1.445 ± 0.053
4.296PheSer: 4.296 ± 0.093
2.913PheThr: 2.913 ± 0.068
2.547PheVal: 2.547 ± 0.065
0.527PheTrp: 0.527 ± 0.028
2.559PheTyr: 2.559 ± 0.065
0.0PheXaa: 0.0 ± 0.0
Gly
3.632GlyAla: 3.632 ± 0.088
0.589GlyCys: 0.589 ± 0.031
2.921GlyAsp: 2.921 ± 0.07
3.658GlyGlu: 3.658 ± 0.086
3.191GlyPhe: 3.191 ± 0.065
4.187GlyGly: 4.187 ± 0.097
0.926GlyHis: 0.926 ± 0.039
5.98GlyIle: 5.98 ± 0.102
5.334GlyLys: 5.334 ± 0.091
5.146GlyLeu: 5.146 ± 0.097
1.423GlyMet: 1.423 ± 0.045
3.548GlyAsn: 3.548 ± 0.076
1.07GlyPro: 1.07 ± 0.041
1.598GlyGln: 1.598 ± 0.046
1.93GlyArg: 1.93 ± 0.063
3.593GlySer: 3.593 ± 0.085
3.203GlyThr: 3.203 ± 0.067
3.848GlyVal: 3.848 ± 0.078
0.702GlyTrp: 0.702 ± 0.034
2.81GlyTyr: 2.81 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
0.849HisAla: 0.849 ± 0.033
0.175HisCys: 0.175 ± 0.018
0.68HisAsp: 0.68 ± 0.028
0.98HisGlu: 0.98 ± 0.032
0.923HisPhe: 0.923 ± 0.031
0.92HisGly: 0.92 ± 0.039
0.339HisHis: 0.339 ± 0.026
1.462HisIle: 1.462 ± 0.052
1.349HisLys: 1.349 ± 0.05
1.864HisLeu: 1.864 ± 0.049
0.32HisMet: 0.32 ± 0.021
0.874HisAsn: 0.874 ± 0.035
0.864HisPro: 0.864 ± 0.037
0.609HisGln: 0.609 ± 0.029
0.58HisArg: 0.58 ± 0.032
1.192HisSer: 1.192 ± 0.039
0.884HisThr: 0.884 ± 0.037
0.739HisVal: 0.739 ± 0.031
0.213HisTrp: 0.213 ± 0.017
0.732HisTyr: 0.732 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.056IleAla: 5.056 ± 0.1
0.827IleCys: 0.827 ± 0.037
4.898IleAsp: 4.898 ± 0.074
6.261IleGlu: 6.261 ± 0.107
4.841IlePhe: 4.841 ± 0.094
5.269IleGly: 5.269 ± 0.1
1.716IleHis: 1.716 ± 0.05
8.189IleIle: 8.189 ± 0.146
7.779IleLys: 7.779 ± 0.119
9.043IleLeu: 9.043 ± 0.141
1.509IleMet: 1.509 ± 0.053
6.122IleAsn: 6.122 ± 0.114
3.833IlePro: 3.833 ± 0.075
3.411IleGln: 3.411 ± 0.084
2.613IleArg: 2.613 ± 0.068
7.082IleSer: 7.082 ± 0.104
4.704IleThr: 4.704 ± 0.078
4.899IleVal: 4.899 ± 0.098
0.662IleTrp: 0.662 ± 0.029
3.869IleTyr: 3.869 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
4.314LysAla: 4.314 ± 0.096
0.42LysCys: 0.42 ± 0.026
5.151LysAsp: 5.151 ± 0.099
7.651LysGlu: 7.651 ± 0.13
3.608LysPhe: 3.608 ± 0.076
4.674LysGly: 4.674 ± 0.088
1.23LysHis: 1.23 ± 0.044
8.893LysIle: 8.893 ± 0.12
8.596LysLys: 8.596 ± 0.142
7.377LysLeu: 7.377 ± 0.111
1.928LysMet: 1.928 ± 0.052
7.339LysAsn: 7.339 ± 0.11
2.45LysPro: 2.45 ± 0.053
3.123LysGln: 3.123 ± 0.065
2.68LysArg: 2.68 ± 0.062
5.158LysSer: 5.158 ± 0.093
4.256LysThr: 4.256 ± 0.074
4.749LysVal: 4.749 ± 0.095
0.655LysTrp: 0.655 ± 0.03
3.973LysTyr: 3.973 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
5.392LeuAla: 5.392 ± 0.096
0.827LeuCys: 0.827 ± 0.033
5.066LeuAsp: 5.066 ± 0.083
6.611LeuGlu: 6.611 ± 0.115
5.084LeuPhe: 5.084 ± 0.121
6.135LeuGly: 6.135 ± 0.117
1.44LeuHis: 1.44 ± 0.044
8.229LeuIle: 8.229 ± 0.12
8.654LeuLys: 8.654 ± 0.112
9.583LeuLeu: 9.583 ± 0.158
2.111LeuMet: 2.111 ± 0.056
6.638LeuAsn: 6.638 ± 0.109
3.602LeuPro: 3.602 ± 0.069
3.247LeuGln: 3.247 ± 0.077
3.063LeuArg: 3.063 ± 0.075
7.269LeuSer: 7.269 ± 0.105
4.96LeuThr: 4.96 ± 0.081
5.205LeuVal: 5.205 ± 0.091
0.91LeuTrp: 0.91 ± 0.034
3.665LeuTyr: 3.665 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
1.262MetAla: 1.262 ± 0.047
0.108MetCys: 0.108 ± 0.013
1.166MetAsp: 1.166 ± 0.037
1.387MetGlu: 1.387 ± 0.044
0.899MetPhe: 0.899 ± 0.041
1.387MetGly: 1.387 ± 0.044
0.349MetHis: 0.349 ± 0.021
1.619MetIle: 1.619 ± 0.045
2.218MetLys: 2.218 ± 0.055
1.734MetLeu: 1.734 ± 0.05
0.47MetMet: 0.47 ± 0.032
1.501MetAsn: 1.501 ± 0.043
0.781MetPro: 0.781 ± 0.032
0.707MetGln: 0.707 ± 0.032
0.725MetArg: 0.725 ± 0.029
1.276MetSer: 1.276 ± 0.044
0.862MetThr: 0.862 ± 0.039
1.198MetVal: 1.198 ± 0.035
0.136MetTrp: 0.136 ± 0.014
0.649MetTyr: 0.649 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.892AsnAla: 2.892 ± 0.067
0.585AsnCys: 0.585 ± 0.034
2.977AsnAsp: 2.977 ± 0.074
4.014AsnGlu: 4.014 ± 0.081
3.691AsnPhe: 3.691 ± 0.08
3.361AsnGly: 3.361 ± 0.082
1.279AsnHis: 1.279 ± 0.039
6.03AsnIle: 6.03 ± 0.088
6.118AsnLys: 6.118 ± 0.097
6.621AsnLeu: 6.621 ± 0.1
1.206AsnMet: 1.206 ± 0.043
4.533AsnAsn: 4.533 ± 0.11
2.941AsnPro: 2.941 ± 0.069
2.825AsnGln: 2.825 ± 0.065
2.072AsnArg: 2.072 ± 0.055
4.795AsnSer: 4.795 ± 0.097
3.355AsnThr: 3.355 ± 0.072
2.952AsnVal: 2.952 ± 0.057
0.7AsnTrp: 0.7 ± 0.032
3.468AsnTyr: 3.468 ± 0.078
0.0AsnXaa: 0.0 ± 0.0
Pro
1.5ProAla: 1.5 ± 0.054
0.271ProCys: 0.271 ± 0.02
1.693ProAsp: 1.693 ± 0.053
2.741ProGlu: 2.741 ± 0.064
1.709ProPhe: 1.709 ± 0.047
1.739ProGly: 1.739 ± 0.049
0.555ProHis: 0.555 ± 0.03
3.167ProIle: 3.167 ± 0.063
2.787ProLys: 2.787 ± 0.063
3.347ProLeu: 3.347 ± 0.068
0.649ProMet: 0.649 ± 0.03
2.143ProAsn: 2.143 ± 0.053
0.908ProPro: 0.908 ± 0.036
1.204ProGln: 1.204 ± 0.043
0.856ProArg: 0.856 ± 0.034
2.385ProSer: 2.385 ± 0.051
1.876ProThr: 1.876 ± 0.046
2.149ProVal: 2.149 ± 0.059
0.366ProTrp: 0.366 ± 0.021
1.533ProTyr: 1.533 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
1.997GlnAla: 1.997 ± 0.063
0.182GlnCys: 0.182 ± 0.017
1.502GlnAsp: 1.502 ± 0.044
2.798GlnGlu: 2.798 ± 0.065
1.444GlnPhe: 1.444 ± 0.046
2.035GlnGly: 2.035 ± 0.066
0.506GlnHis: 0.506 ± 0.028
3.123GlnIle: 3.123 ± 0.067
3.335GlnLys: 3.335 ± 0.07
3.527GlnLeu: 3.527 ± 0.075
0.824GlnMet: 0.824 ± 0.034
2.31GlnAsn: 2.31 ± 0.06
1.06GlnPro: 1.06 ± 0.043
1.361GlnGln: 1.361 ± 0.051
1.244GlnArg: 1.244 ± 0.046
2.019GlnSer: 2.019 ± 0.053
1.961GlnThr: 1.961 ± 0.06
1.968GlnVal: 1.968 ± 0.047
0.491GlnTrp: 0.491 ± 0.026
1.479GlnTyr: 1.479 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
1.65ArgAla: 1.65 ± 0.053
0.247ArgCys: 0.247 ± 0.02
1.47ArgAsp: 1.47 ± 0.047
2.204ArgGlu: 2.204 ± 0.06
1.589ArgPhe: 1.589 ± 0.052
1.759ArgGly: 1.759 ± 0.053
0.432ArgHis: 0.432 ± 0.023
3.037ArgIle: 3.037 ± 0.068
2.867ArgLys: 2.867 ± 0.066
3.019ArgLeu: 3.019 ± 0.072
0.828ArgMet: 0.828 ± 0.034
2.062ArgAsn: 2.062 ± 0.054
0.919ArgPro: 0.919 ± 0.035
0.953ArgGln: 0.953 ± 0.039
1.19ArgArg: 1.19 ± 0.041
1.791ArgSer: 1.791 ± 0.059
1.484ArgThr: 1.484 ± 0.047
1.914ArgVal: 1.914 ± 0.06
0.35ArgTrp: 0.35 ± 0.025
1.495ArgTyr: 1.495 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.13SerAla: 3.13 ± 0.071
0.73SerCys: 0.73 ± 0.037
3.224SerAsp: 3.224 ± 0.058
4.104SerGlu: 4.104 ± 0.09
3.916SerPhe: 3.916 ± 0.071
4.048SerGly: 4.048 ± 0.077
1.106SerHis: 1.106 ± 0.044
6.66SerIle: 6.66 ± 0.111
5.802SerLys: 5.802 ± 0.085
7.251SerLeu: 7.251 ± 0.108
1.39SerMet: 1.39 ± 0.042
4.232SerAsn: 4.232 ± 0.071
2.278SerPro: 2.278 ± 0.061
2.468SerGln: 2.468 ± 0.069
1.896SerArg: 1.896 ± 0.055
4.835SerSer: 4.835 ± 0.095
3.563SerThr: 3.563 ± 0.077
3.498SerVal: 3.498 ± 0.079
0.62SerTrp: 0.62 ± 0.031
3.001SerTyr: 3.001 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
2.689ThrAla: 2.689 ± 0.063
0.399ThrCys: 0.399 ± 0.023
2.613ThrAsp: 2.613 ± 0.063
3.153ThrGlu: 3.153 ± 0.066
2.702ThrPhe: 2.702 ± 0.066
3.355ThrGly: 3.355 ± 0.078
1.019ThrHis: 1.019 ± 0.034
4.791ThrIle: 4.791 ± 0.087
3.598ThrLys: 3.598 ± 0.068
5.252ThrLeu: 5.252 ± 0.087
0.851ThrMet: 0.851 ± 0.034
2.995ThrAsn: 2.995 ± 0.074
2.179ThrPro: 2.179 ± 0.061
1.992ThrGln: 1.992 ± 0.055
1.461ThrArg: 1.461 ± 0.05
3.59ThrSer: 3.59 ± 0.067
2.869ThrThr: 2.869 ± 0.077
2.763ThrVal: 2.763 ± 0.061
0.457ThrTrp: 0.457 ± 0.027
2.404ThrTyr: 2.404 ± 0.07
0.0ThrXaa: 0.0 ± 0.0
Val
3.064ValAla: 3.064 ± 0.064
0.543ValCys: 0.543 ± 0.03
2.996ValAsp: 2.996 ± 0.074
3.679ValGlu: 3.679 ± 0.078
2.817ValPhe: 2.817 ± 0.063
3.515ValGly: 3.515 ± 0.085
0.87ValHis: 0.87 ± 0.042
4.686ValIle: 4.686 ± 0.103
4.403ValLys: 4.403 ± 0.087
5.07ValLeu: 5.07 ± 0.093
1.123ValMet: 1.123 ± 0.041
3.466ValAsn: 3.466 ± 0.074
2.005ValPro: 2.005 ± 0.059
1.764ValGln: 1.764 ± 0.057
1.714ValArg: 1.714 ± 0.049
3.825ValSer: 3.825 ± 0.08
2.695ValThr: 2.695 ± 0.063
3.518ValVal: 3.518 ± 0.092
0.573ValTrp: 0.573 ± 0.03
2.363ValTyr: 2.363 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.523TrpAla: 0.523 ± 0.029
0.083TrpCys: 0.083 ± 0.012
0.538TrpAsp: 0.538 ± 0.03
0.668TrpGlu: 0.668 ± 0.029
0.502TrpPhe: 0.502 ± 0.03
0.678TrpGly: 0.678 ± 0.037
0.157TrpHis: 0.157 ± 0.014
0.831TrpIle: 0.831 ± 0.037
0.905TrpLys: 0.905 ± 0.04
0.98TrpLeu: 0.98 ± 0.039
0.29TrpMet: 0.29 ± 0.018
0.735TrpAsn: 0.735 ± 0.031
0.165TrpPro: 0.165 ± 0.014
0.331TrpGln: 0.331 ± 0.018
0.325TrpArg: 0.325 ± 0.024
0.559TrpSer: 0.559 ± 0.029
0.425TrpThr: 0.425 ± 0.025
0.614TrpVal: 0.614 ± 0.028
0.121TrpTrp: 0.121 ± 0.013
0.409TrpTyr: 0.409 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.132TyrAla: 2.132 ± 0.057
0.367TyrCys: 0.367 ± 0.02
2.208TyrAsp: 2.208 ± 0.079
2.539TyrGlu: 2.539 ± 0.059
2.613TyrPhe: 2.613 ± 0.075
2.514TyrGly: 2.514 ± 0.066
0.832TyrHis: 0.832 ± 0.033
3.673TyrIle: 3.673 ± 0.08
3.997TyrLys: 3.997 ± 0.079
4.682TyrLeu: 4.682 ± 0.092
0.767TyrMet: 0.767 ± 0.035
2.973TyrAsn: 2.973 ± 0.077
1.691TyrPro: 1.691 ± 0.054
1.751TyrGln: 1.751 ± 0.05
1.545TyrArg: 1.545 ± 0.053
3.284TyrSer: 3.284 ± 0.078
2.495TyrThr: 2.495 ± 0.073
2.092TyrVal: 2.092 ± 0.055
0.525TyrTrp: 0.525 ± 0.032
2.235TyrTyr: 2.235 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2183 proteins (719535 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski