Vibrio phage ICP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Icepovirus; Vibrio virus ICP2

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1D0Q8|F1D0Q8_9CAUD Putative DNA helicase OS=Vibrio phage ICP2 OX=979533 GN=49 PE=4 SV=1
MM1 pKa = 7.87GEE3 pKa = 4.17EE4 pKa = 4.07IKK6 pKa = 11.24VGDD9 pKa = 3.72WVRR12 pKa = 11.84YY13 pKa = 5.99ITSGGPDD20 pKa = 3.05LTHH23 pKa = 6.42NKK25 pKa = 9.22EE26 pKa = 3.88YY27 pKa = 10.82QIIQVYY33 pKa = 10.58SSDD36 pKa = 3.0ATYY39 pKa = 11.29GMIRR43 pKa = 11.84VLDD46 pKa = 4.04DD47 pKa = 3.41VGDD50 pKa = 3.77VHH52 pKa = 9.13DD53 pKa = 5.72LMDD56 pKa = 6.26FEE58 pKa = 5.05YY59 pKa = 10.67EE60 pKa = 3.93WLGTRR65 pKa = 11.84NPDD68 pKa = 3.36WILVFQKK75 pKa = 11.12GDD77 pKa = 3.35TSPNGGEE84 pKa = 4.0WASLTVFDD92 pKa = 6.34NEE94 pKa = 4.49TQWYY98 pKa = 8.35CFRR101 pKa = 11.84NIEE104 pKa = 4.06GEE106 pKa = 4.08LRR108 pKa = 11.84FVSDD112 pKa = 3.61SPATKK117 pKa = 10.22EE118 pKa = 3.69PLDD121 pKa = 3.86AVRR124 pKa = 11.84AAAMLLVFADD134 pKa = 4.68EE135 pKa = 4.15EE136 pKa = 4.42AKK138 pKa = 10.83LL139 pKa = 3.68

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1D0P1|F1D0P1_9CAUD Uncharacterized protein 32 OS=Vibrio phage ICP2 OX=979533 GN=32 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.23QRR4 pKa = 11.84NPNRR8 pKa = 11.84AHH10 pKa = 5.66SPEE13 pKa = 3.78RR14 pKa = 11.84LMLRR18 pKa = 11.84KK19 pKa = 8.35QTADD23 pKa = 2.91VGLIKK28 pKa = 10.53VVNLISRR35 pKa = 11.84ATGGAVFGGRR45 pKa = 11.84LGFICAAIKK54 pKa = 10.24QDD56 pKa = 3.25GANIRR61 pKa = 11.84SLSTWEE67 pKa = 4.86AYY69 pKa = 9.18GQVYY73 pKa = 10.82DD74 pKa = 4.53SVTTMAIVHH83 pKa = 6.59

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

15451

25

1416

214.6

23.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.689 ± 0.492

1.042 ± 0.173

6.071 ± 0.256

6.796 ± 0.266

3.773 ± 0.194

7.631 ± 0.446

1.527 ± 0.168

5.637 ± 0.18

6.271 ± 0.26

7.514 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.223

4.873 ± 0.217

3.903 ± 0.27

4.142 ± 0.327

5.055 ± 0.231

6.42 ± 0.339

6.459 ± 0.276

7.016 ± 0.273

1.521 ± 0.116

3.773 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski