Vibrio phage ICP2
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1D0Q8|F1D0Q8_9CAUD Putative DNA helicase OS=Vibrio phage ICP2 OX=979533 GN=49 PE=4 SV=1
MM1 pKa = 7.87 GEE3 pKa = 4.17 EE4 pKa = 4.07 IKK6 pKa = 11.24 VGDD9 pKa = 3.72 WVRR12 pKa = 11.84 YY13 pKa = 5.99 ITSGGPDD20 pKa = 3.05 LTHH23 pKa = 6.42 NKK25 pKa = 9.22 EE26 pKa = 3.88 YY27 pKa = 10.82 QIIQVYY33 pKa = 10.58 SSDD36 pKa = 3.0 ATYY39 pKa = 11.29 GMIRR43 pKa = 11.84 VLDD46 pKa = 4.04 DD47 pKa = 3.41 VGDD50 pKa = 3.77 VHH52 pKa = 9.13 DD53 pKa = 5.72 LMDD56 pKa = 6.26 FEE58 pKa = 5.05 YY59 pKa = 10.67 EE60 pKa = 3.93 WLGTRR65 pKa = 11.84 NPDD68 pKa = 3.36 WILVFQKK75 pKa = 11.12 GDD77 pKa = 3.35 TSPNGGEE84 pKa = 4.0 WASLTVFDD92 pKa = 6.34 NEE94 pKa = 4.49 TQWYY98 pKa = 8.35 CFRR101 pKa = 11.84 NIEE104 pKa = 4.06 GEE106 pKa = 4.08 LRR108 pKa = 11.84 FVSDD112 pKa = 3.61 SPATKK117 pKa = 10.22 EE118 pKa = 3.69 PLDD121 pKa = 3.86 AVRR124 pKa = 11.84 AAAMLLVFADD134 pKa = 4.68 EE135 pKa = 4.15 EE136 pKa = 4.42 AKK138 pKa = 10.83 LL139 pKa = 3.68
Molecular weight: 15.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.258
IPC2_protein 4.164
IPC_protein 4.113
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.999
Rodwell 3.948
Grimsley 3.834
Solomon 4.088
Lehninger 4.037
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.24
Patrickios 3.554
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.151
Protein with the highest isoelectric point:
>tr|F1D0P1|F1D0P1_9CAUD Uncharacterized protein 32 OS=Vibrio phage ICP2 OX=979533 GN=32 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.23 QRR4 pKa = 11.84 NPNRR8 pKa = 11.84 AHH10 pKa = 5.66 SPEE13 pKa = 3.78 RR14 pKa = 11.84 LMLRR18 pKa = 11.84 KK19 pKa = 8.35 QTADD23 pKa = 2.91 VGLIKK28 pKa = 10.53 VVNLISRR35 pKa = 11.84 ATGGAVFGGRR45 pKa = 11.84 LGFICAAIKK54 pKa = 10.24 QDD56 pKa = 3.25 GANIRR61 pKa = 11.84 SLSTWEE67 pKa = 4.86 AYY69 pKa = 9.18 GQVYY73 pKa = 10.82 DD74 pKa = 4.53 SVTTMAIVHH83 pKa = 6.59
Molecular weight: 9.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.575
IPC_protein 10.101
Toseland 10.467
ProMoST 10.116
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.906
Grimsley 10.657
Solomon 10.672
Lehninger 10.657
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 9.18
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
15451
25
1416
214.6
23.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.689 ± 0.492
1.042 ± 0.173
6.071 ± 0.256
6.796 ± 0.266
3.773 ± 0.194
7.631 ± 0.446
1.527 ± 0.168
5.637 ± 0.18
6.271 ± 0.26
7.514 ± 0.292
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.887 ± 0.223
4.873 ± 0.217
3.903 ± 0.27
4.142 ± 0.327
5.055 ± 0.231
6.42 ± 0.339
6.459 ± 0.276
7.016 ± 0.273
1.521 ± 0.116
3.773 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here