Amino acid dipepetide frequency for Paenibacillus graminis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.726AlaAla: 10.726 ± 0.119
0.728AlaCys: 0.728 ± 0.025
4.464AlaAsp: 4.464 ± 0.059
6.352AlaGlu: 6.352 ± 0.076
3.319AlaPhe: 3.319 ± 0.048
8.029AlaGly: 8.029 ± 0.078
1.389AlaHis: 1.389 ± 0.031
4.854AlaIle: 4.854 ± 0.064
4.036AlaLys: 4.036 ± 0.055
8.607AlaLeu: 8.607 ± 0.081
2.227AlaMet: 2.227 ± 0.039
2.444AlaAsn: 2.444 ± 0.045
3.007AlaPro: 3.007 ± 0.049
2.702AlaGln: 2.702 ± 0.039
3.685AlaArg: 3.685 ± 0.049
5.172AlaSer: 5.172 ± 0.069
3.482AlaThr: 3.482 ± 0.056
7.254AlaVal: 7.254 ± 0.07
0.967AlaTrp: 0.967 ± 0.024
2.805AlaTyr: 2.805 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.557CysAla: 0.557 ± 0.018
0.114CysCys: 0.114 ± 0.009
0.374CysAsp: 0.374 ± 0.013
0.407CysGlu: 0.407 ± 0.017
0.323CysPhe: 0.323 ± 0.014
0.812CysGly: 0.812 ± 0.023
0.194CysHis: 0.194 ± 0.012
0.533CysIle: 0.533 ± 0.019
0.314CysLys: 0.314 ± 0.014
0.804CysLeu: 0.804 ± 0.024
0.223CysMet: 0.223 ± 0.012
0.276CysAsn: 0.276 ± 0.013
0.348CysPro: 0.348 ± 0.014
0.243CysGln: 0.243 ± 0.012
0.529CysArg: 0.529 ± 0.018
0.591CysSer: 0.591 ± 0.018
0.4CysThr: 0.4 ± 0.015
0.415CysVal: 0.415 ± 0.016
0.111CysTrp: 0.111 ± 0.008
0.282CysTyr: 0.282 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.661AspAla: 3.661 ± 0.052
0.379AspCys: 0.379 ± 0.014
2.196AspAsp: 2.196 ± 0.046
3.483AspGlu: 3.483 ± 0.047
2.147AspPhe: 2.147 ± 0.038
3.912AspGly: 3.912 ± 0.056
1.051AspHis: 1.051 ± 0.026
3.592AspIle: 3.592 ± 0.05
2.786AspLys: 2.786 ± 0.044
4.759AspLeu: 4.759 ± 0.047
1.337AspMet: 1.337 ± 0.028
1.889AspAsn: 1.889 ± 0.033
2.308AspPro: 2.308 ± 0.043
1.713AspGln: 1.713 ± 0.032
2.474AspArg: 2.474 ± 0.044
2.956AspSer: 2.956 ± 0.043
2.585AspThr: 2.585 ± 0.037
3.117AspVal: 3.117 ± 0.04
0.803AspTrp: 0.803 ± 0.023
2.128AspTyr: 2.128 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.255GluAla: 6.255 ± 0.07
0.411GluCys: 0.411 ± 0.016
3.1GluAsp: 3.1 ± 0.044
5.35GluGlu: 5.35 ± 0.066
2.196GluPhe: 2.196 ± 0.037
4.572GluGly: 4.572 ± 0.056
1.562GluHis: 1.562 ± 0.03
4.446GluIle: 4.446 ± 0.052
3.543GluLys: 3.543 ± 0.051
7.382GluLeu: 7.382 ± 0.075
2.059GluMet: 2.059 ± 0.039
2.444GluAsn: 2.444 ± 0.035
2.318GluPro: 2.318 ± 0.037
3.487GluGln: 3.487 ± 0.059
3.846GluArg: 3.846 ± 0.053
3.395GluSer: 3.395 ± 0.042
3.168GluThr: 3.168 ± 0.05
4.349GluVal: 4.349 ± 0.05
0.908GluTrp: 0.908 ± 0.023
2.157GluTyr: 2.157 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.433PheAla: 3.433 ± 0.051
0.386PheCys: 0.386 ± 0.015
2.063PheAsp: 2.063 ± 0.039
2.241PheGlu: 2.241 ± 0.038
1.869PhePhe: 1.869 ± 0.038
3.275PheGly: 3.275 ± 0.051
0.866PheHis: 0.866 ± 0.018
2.855PheIle: 2.855 ± 0.045
1.939PheLys: 1.939 ± 0.034
3.902PheLeu: 3.902 ± 0.059
1.194PheMet: 1.194 ± 0.029
1.611PheAsn: 1.611 ± 0.035
1.649PhePro: 1.649 ± 0.03
1.428PheGln: 1.428 ± 0.027
2.054PheArg: 2.054 ± 0.032
2.986PheSer: 2.986 ± 0.045
2.545PheThr: 2.545 ± 0.036
2.729PheVal: 2.729 ± 0.043
0.56PheTrp: 0.56 ± 0.02
1.529PheTyr: 1.529 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
5.972GlyAla: 5.972 ± 0.079
0.747GlyCys: 0.747 ± 0.023
3.548GlyAsp: 3.548 ± 0.047
4.763GlyGlu: 4.763 ± 0.053
3.297GlyPhe: 3.297 ± 0.045
6.133GlyGly: 6.133 ± 0.066
1.541GlyHis: 1.541 ± 0.03
5.895GlyIle: 5.895 ± 0.057
4.506GlyLys: 4.506 ± 0.056
7.834GlyLeu: 7.834 ± 0.084
2.471GlyMet: 2.471 ± 0.042
2.964GlyAsn: 2.964 ± 0.046
2.111GlyPro: 2.111 ± 0.041
2.818GlyGln: 2.818 ± 0.041
3.802GlyArg: 3.802 ± 0.055
5.1GlySer: 5.1 ± 0.069
4.587GlyThr: 4.587 ± 0.062
5.346GlyVal: 5.346 ± 0.062
1.05GlyTrp: 1.05 ± 0.025
3.081GlyTyr: 3.081 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
1.484HisAla: 1.484 ± 0.034
0.21HisCys: 0.21 ± 0.011
0.891HisAsp: 0.891 ± 0.023
1.263HisGlu: 1.263 ± 0.024
1.01HisPhe: 1.01 ± 0.025
1.519HisGly: 1.519 ± 0.033
0.541HisHis: 0.541 ± 0.019
1.346HisIle: 1.346 ± 0.028
0.904HisLys: 0.904 ± 0.024
2.098HisLeu: 2.098 ± 0.059
0.576HisMet: 0.576 ± 0.017
0.74HisAsn: 0.74 ± 0.021
1.236HisPro: 1.236 ± 0.029
0.718HisGln: 0.718 ± 0.021
1.062HisArg: 1.062 ± 0.025
1.303HisSer: 1.303 ± 0.029
1.167HisThr: 1.167 ± 0.025
1.245HisVal: 1.245 ± 0.029
0.299HisTrp: 0.299 ± 0.013
0.844HisTyr: 0.844 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.816IleAla: 5.816 ± 0.053
0.599IleCys: 0.599 ± 0.017
3.306IleAsp: 3.306 ± 0.048
3.886IleGlu: 3.886 ± 0.053
2.51IlePhe: 2.51 ± 0.036
5.093IleGly: 5.093 ± 0.063
1.471IleHis: 1.471 ± 0.027
4.363IleIle: 4.363 ± 0.059
2.906IleLys: 2.906 ± 0.042
6.091IleLeu: 6.091 ± 0.064
1.663IleMet: 1.663 ± 0.027
2.383IleAsn: 2.383 ± 0.043
3.177IlePro: 3.177 ± 0.045
2.402IleGln: 2.402 ± 0.042
3.654IleArg: 3.654 ± 0.047
4.701IleSer: 4.701 ± 0.061
4.0IleThr: 4.0 ± 0.049
4.704IleVal: 4.704 ± 0.057
0.655IleTrp: 0.655 ± 0.02
2.122IleTyr: 2.122 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.303LysAla: 4.303 ± 0.054
0.276LysCys: 0.276 ± 0.012
2.873LysAsp: 2.873 ± 0.049
4.083LysGlu: 4.083 ± 0.057
1.559LysPhe: 1.559 ± 0.029
3.631LysGly: 3.631 ± 0.053
0.988LysHis: 0.988 ± 0.026
3.139LysIle: 3.139 ± 0.048
3.094LysLys: 3.094 ± 0.044
5.552LysLeu: 5.552 ± 0.063
1.496LysMet: 1.496 ± 0.031
2.075LysAsn: 2.075 ± 0.034
2.114LysPro: 2.114 ± 0.034
2.233LysGln: 2.233 ± 0.036
2.665LysArg: 2.665 ± 0.041
2.867LysSer: 2.867 ± 0.041
2.694LysThr: 2.694 ± 0.038
3.552LysVal: 3.552 ± 0.048
0.638LysTrp: 0.638 ± 0.018
1.851LysTyr: 1.851 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
8.86LeuAla: 8.86 ± 0.092
0.879LeuCys: 0.879 ± 0.024
5.167LeuAsp: 5.167 ± 0.06
6.705LeuGlu: 6.705 ± 0.075
4.56LeuPhe: 4.56 ± 0.06
7.219LeuGly: 7.219 ± 0.074
2.16LeuHis: 2.16 ± 0.032
6.422LeuIle: 6.422 ± 0.074
5.461LeuLys: 5.461 ± 0.065
12.051LeuLeu: 12.051 ± 0.129
2.752LeuMet: 2.752 ± 0.04
3.976LeuAsn: 3.976 ± 0.055
4.936LeuPro: 4.936 ± 0.061
4.37LeuGln: 4.37 ± 0.055
5.181LeuArg: 5.181 ± 0.061
7.411LeuSer: 7.411 ± 0.067
5.69LeuThr: 5.69 ± 0.056
6.23LeuVal: 6.23 ± 0.062
1.082LeuTrp: 1.082 ± 0.027
3.378LeuTyr: 3.378 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.23MetAla: 2.23 ± 0.037
0.162MetCys: 0.162 ± 0.01
1.537MetAsp: 1.537 ± 0.03
1.933MetGlu: 1.933 ± 0.029
1.034MetPhe: 1.034 ± 0.027
1.81MetGly: 1.81 ± 0.035
0.447MetHis: 0.447 ± 0.017
1.871MetIle: 1.871 ± 0.036
1.909MetLys: 1.909 ± 0.032
3.05MetLeu: 3.05 ± 0.046
0.819MetMet: 0.819 ± 0.026
1.505MetAsn: 1.505 ± 0.028
1.149MetPro: 1.149 ± 0.034
0.968MetGln: 0.968 ± 0.023
1.23MetArg: 1.23 ± 0.023
1.774MetSer: 1.774 ± 0.032
1.583MetThr: 1.583 ± 0.032
1.687MetVal: 1.687 ± 0.025
0.194MetTrp: 0.194 ± 0.011
0.799MetTyr: 0.799 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.92AsnAla: 2.92 ± 0.047
0.268AsnCys: 0.268 ± 0.012
1.785AsnAsp: 1.785 ± 0.033
2.308AsnGlu: 2.308 ± 0.032
1.434AsnPhe: 1.434 ± 0.031
3.237AsnGly: 3.237 ± 0.057
0.835AsnHis: 0.835 ± 0.023
2.476AsnIle: 2.476 ± 0.042
1.997AsnLys: 1.997 ± 0.032
3.494AsnLeu: 3.494 ± 0.046
0.982AsnMet: 0.982 ± 0.024
1.713AsnAsn: 1.713 ± 0.04
2.045AsnPro: 2.045 ± 0.035
1.347AsnGln: 1.347 ± 0.03
1.949AsnArg: 1.949 ± 0.036
2.264AsnSer: 2.264 ± 0.042
2.119AsnThr: 2.119 ± 0.041
2.487AsnVal: 2.487 ± 0.04
0.506AsnTrp: 0.506 ± 0.018
1.41AsnTyr: 1.41 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
3.944ProAla: 3.944 ± 0.064
0.261ProCys: 0.261 ± 0.013
2.523ProAsp: 2.523 ± 0.043
3.666ProGlu: 3.666 ± 0.051
1.881ProPhe: 1.881 ± 0.041
3.54ProGly: 3.54 ± 0.051
0.813ProHis: 0.813 ± 0.021
2.003ProIle: 2.003 ± 0.035
1.708ProLys: 1.708 ± 0.032
4.39ProLeu: 4.39 ± 0.059
0.911ProMet: 0.911 ± 0.022
1.32ProAsn: 1.32 ± 0.03
1.353ProPro: 1.353 ± 0.038
1.657ProGln: 1.657 ± 0.041
1.472ProArg: 1.472 ± 0.034
2.467ProSer: 2.467 ± 0.041
1.699ProThr: 1.699 ± 0.044
3.644ProVal: 3.644 ± 0.046
0.49ProTrp: 0.49 ± 0.015
1.546ProTyr: 1.546 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.46GlnAla: 3.46 ± 0.048
0.212GlnCys: 0.212 ± 0.012
1.663GlnAsp: 1.663 ± 0.034
2.791GlnGlu: 2.791 ± 0.046
1.436GlnPhe: 1.436 ± 0.031
2.759GlnGly: 2.759 ± 0.046
0.814GlnHis: 0.814 ± 0.021
2.478GlnIle: 2.478 ± 0.042
2.039GlnLys: 2.039 ± 0.033
4.106GlnLeu: 4.106 ± 0.049
1.135GlnMet: 1.135 ± 0.022
1.377GlnAsn: 1.377 ± 0.027
1.585GlnPro: 1.585 ± 0.032
1.851GlnGln: 1.851 ± 0.035
1.944GlnArg: 1.944 ± 0.034
2.215GlnSer: 2.215 ± 0.038
1.931GlnThr: 1.931 ± 0.03
2.385GlnVal: 2.385 ± 0.037
0.475GlnTrp: 0.475 ± 0.016
1.344GlnTyr: 1.344 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
3.288ArgAla: 3.288 ± 0.045
0.369ArgCys: 0.369 ± 0.016
2.385ArgAsp: 2.385 ± 0.038
3.834ArgGlu: 3.834 ± 0.056
2.076ArgPhe: 2.076 ± 0.035
3.089ArgGly: 3.089 ± 0.043
1.122ArgHis: 1.122 ± 0.03
3.58ArgIle: 3.58 ± 0.049
3.05ArgLys: 3.05 ± 0.041
5.455ArgLeu: 5.455 ± 0.063
1.54ArgMet: 1.54 ± 0.034
2.01ArgAsn: 2.01 ± 0.039
1.719ArgPro: 1.719 ± 0.038
2.166ArgGln: 2.166 ± 0.037
2.888ArgArg: 2.888 ± 0.048
3.262ArgSer: 3.262 ± 0.047
2.713ArgThr: 2.713 ± 0.036
3.029ArgVal: 3.029 ± 0.039
0.607ArgTrp: 0.607 ± 0.018
1.927ArgTyr: 1.927 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
5.371SerAla: 5.371 ± 0.062
0.47SerCys: 0.47 ± 0.017
2.919SerAsp: 2.919 ± 0.046
3.627SerGlu: 3.627 ± 0.051
3.034SerPhe: 3.034 ± 0.047
6.168SerGly: 6.168 ± 0.066
1.216SerHis: 1.216 ± 0.029
4.306SerIle: 4.306 ± 0.055
3.081SerLys: 3.081 ± 0.04
6.806SerLeu: 6.806 ± 0.069
1.712SerMet: 1.712 ± 0.027
2.148SerAsn: 2.148 ± 0.037
2.661SerPro: 2.661 ± 0.044
1.991SerGln: 1.991 ± 0.032
3.43SerArg: 3.43 ± 0.045
4.566SerSer: 4.566 ± 0.066
3.097SerThr: 3.097 ± 0.049
4.548SerVal: 4.548 ± 0.058
0.817SerTrp: 0.817 ± 0.022
2.317SerTyr: 2.317 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
5.317ThrAla: 5.317 ± 0.07
0.334ThrCys: 0.334 ± 0.014
2.663ThrAsp: 2.663 ± 0.041
3.132ThrGlu: 3.132 ± 0.043
2.276ThrPhe: 2.276 ± 0.037
4.899ThrGly: 4.899 ± 0.057
1.016ThrHis: 1.016 ± 0.027
3.32ThrIle: 3.32 ± 0.048
2.232ThrLys: 2.232 ± 0.035
5.648ThrLeu: 5.648 ± 0.066
1.254ThrMet: 1.254 ± 0.028
1.683ThrAsn: 1.683 ± 0.037
2.651ThrPro: 2.651 ± 0.047
1.507ThrGln: 1.507 ± 0.03
2.327ThrArg: 2.327 ± 0.035
3.23ThrSer: 3.23 ± 0.047
2.777ThrThr: 2.777 ± 0.046
4.557ThrVal: 4.557 ± 0.06
0.577ThrTrp: 0.577 ± 0.02
1.762ThrTyr: 1.762 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
5.088ValAla: 5.088 ± 0.054
0.626ValCys: 0.626 ± 0.018
3.325ValAsp: 3.325 ± 0.05
4.173ValGlu: 4.173 ± 0.06
2.995ValPhe: 2.995 ± 0.049
4.246ValGly: 4.246 ± 0.055
1.388ValHis: 1.388 ± 0.029
4.969ValIle: 4.969 ± 0.058
3.755ValLys: 3.755 ± 0.048
7.619ValLeu: 7.619 ± 0.075
1.987ValMet: 1.987 ± 0.035
2.856ValAsn: 2.856 ± 0.05
3.014ValPro: 3.014 ± 0.047
2.677ValGln: 2.677 ± 0.043
3.287ValArg: 3.287 ± 0.046
4.768ValSer: 4.768 ± 0.057
4.274ValThr: 4.274 ± 0.066
4.771ValVal: 4.771 ± 0.066
0.811ValTrp: 0.811 ± 0.019
2.432ValTyr: 2.432 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.803TrpAla: 0.803 ± 0.021
0.101TrpCys: 0.101 ± 0.007
0.648TrpAsp: 0.648 ± 0.02
0.749TrpGlu: 0.749 ± 0.021
0.516TrpPhe: 0.516 ± 0.017
0.862TrpGly: 0.862 ± 0.02
0.248TrpHis: 0.248 ± 0.012
0.806TrpIle: 0.806 ± 0.02
0.723TrpLys: 0.723 ± 0.019
1.334TrpLeu: 1.334 ± 0.03
0.397TrpMet: 0.397 ± 0.016
0.716TrpAsn: 0.716 ± 0.023
0.356TrpPro: 0.356 ± 0.014
0.48TrpGln: 0.48 ± 0.016
0.585TrpArg: 0.585 ± 0.019
0.84TrpSer: 0.84 ± 0.022
0.678TrpThr: 0.678 ± 0.023
0.757TrpVal: 0.757 ± 0.02
0.169TrpTrp: 0.169 ± 0.011
0.384TrpTyr: 0.384 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.76TyrAla: 2.76 ± 0.039
0.319TyrCys: 0.319 ± 0.013
1.844TyrAsp: 1.844 ± 0.028
2.204TyrGlu: 2.204 ± 0.035
1.641TyrPhe: 1.641 ± 0.034
2.762TyrGly: 2.762 ± 0.039
0.794TyrHis: 0.794 ± 0.021
2.247TyrIle: 2.247 ± 0.034
1.698TyrLys: 1.698 ± 0.029
3.508TyrLeu: 3.508 ± 0.043
0.957TyrMet: 0.957 ± 0.027
1.449TyrAsn: 1.449 ± 0.036
1.553TyrPro: 1.553 ± 0.034
1.241TyrGln: 1.241 ± 0.031
2.045TyrArg: 2.045 ± 0.032
2.418TyrSer: 2.418 ± 0.041
1.971TyrThr: 1.971 ± 0.034
2.254TyrVal: 2.254 ± 0.042
0.433TyrTrp: 0.433 ± 0.016
1.44TyrTyr: 1.44 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5576 proteins (1797145 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski