Amino acid dipepetide frequency for Lactobacillus ceti DSM 22408

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.258AlaAla: 6.258 ± 0.181
0.466AlaCys: 0.466 ± 0.034
4.565AlaAsp: 4.565 ± 0.118
5.182AlaGlu: 5.182 ± 0.137
2.978AlaPhe: 2.978 ± 0.109
5.162AlaGly: 5.162 ± 0.133
1.26AlaHis: 1.26 ± 0.055
5.445AlaIle: 5.445 ± 0.129
5.822AlaLys: 5.822 ± 0.159
7.508AlaLeu: 7.508 ± 0.156
1.969AlaMet: 1.969 ± 0.069
3.288AlaAsn: 3.288 ± 0.091
2.184AlaPro: 2.184 ± 0.086
4.421AlaGln: 4.421 ± 0.165
2.757AlaArg: 2.757 ± 0.093
3.541AlaSer: 3.541 ± 0.097
4.461AlaThr: 4.461 ± 0.124
5.299AlaVal: 5.299 ± 0.14
0.603AlaTrp: 0.603 ± 0.038
2.784AlaTyr: 2.784 ± 0.094
0.0AlaXaa: 0.0 ± 0.0
Cys
0.466CysAla: 0.466 ± 0.034
0.072CysCys: 0.072 ± 0.014
0.402CysAsp: 0.402 ± 0.029
0.426CysGlu: 0.426 ± 0.036
0.28CysPhe: 0.28 ± 0.034
0.536CysGly: 0.536 ± 0.036
0.179CysHis: 0.179 ± 0.019
0.394CysIle: 0.394 ± 0.031
0.325CysLys: 0.325 ± 0.033
0.679CysLeu: 0.679 ± 0.048
0.129CysMet: 0.129 ± 0.019
0.248CysAsn: 0.248 ± 0.027
0.327CysPro: 0.327 ± 0.03
0.36CysGln: 0.36 ± 0.028
0.27CysArg: 0.27 ± 0.026
0.367CysSer: 0.367 ± 0.029
0.283CysThr: 0.283 ± 0.03
0.374CysVal: 0.374 ± 0.031
0.084CysTrp: 0.084 ± 0.015
0.342CysTyr: 0.342 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.667AspAla: 3.667 ± 0.119
0.369AspCys: 0.369 ± 0.032
2.819AspAsp: 2.819 ± 0.108
4.25AspGlu: 4.25 ± 0.137
2.908AspPhe: 2.908 ± 0.085
3.196AspGly: 3.196 ± 0.107
1.148AspHis: 1.148 ± 0.058
4.056AspIle: 4.056 ± 0.113
3.754AspLys: 3.754 ± 0.106
6.246AspLeu: 6.246 ± 0.119
1.381AspMet: 1.381 ± 0.06
2.41AspAsn: 2.41 ± 0.089
2.125AspPro: 2.125 ± 0.071
2.804AspGln: 2.804 ± 0.089
2.033AspArg: 2.033 ± 0.072
2.289AspSer: 2.289 ± 0.075
2.415AspThr: 2.415 ± 0.075
4.138AspVal: 4.138 ± 0.123
0.538AspTrp: 0.538 ± 0.031
2.554AspTyr: 2.554 ± 0.081
0.002AspXaa: 0.002 ± 0.002
Glu
4.589GluAla: 4.589 ± 0.131
0.369GluCys: 0.369 ± 0.03
3.27GluAsp: 3.27 ± 0.099
4.756GluGlu: 4.756 ± 0.171
2.817GluPhe: 2.817 ± 0.103
2.849GluGly: 2.849 ± 0.102
1.257GluHis: 1.257 ± 0.054
5.755GluIle: 5.755 ± 0.119
6.01GluLys: 6.01 ± 0.136
7.25GluLeu: 7.25 ± 0.163
2.155GluMet: 2.155 ± 0.076
3.657GluAsn: 3.657 ± 0.114
1.832GluPro: 1.832 ± 0.068
3.412GluGln: 3.412 ± 0.118
2.846GluArg: 2.846 ± 0.109
2.633GluSer: 2.633 ± 0.097
3.454GluThr: 3.454 ± 0.11
4.728GluVal: 4.728 ± 0.138
0.501GluTrp: 0.501 ± 0.04
2.536GluTyr: 2.536 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
2.993PheAla: 2.993 ± 0.084
0.327PheCys: 0.327 ± 0.028
2.358PheAsp: 2.358 ± 0.085
2.455PheGlu: 2.455 ± 0.088
1.874PhePhe: 1.874 ± 0.076
2.921PheGly: 2.921 ± 0.079
0.62PheHis: 0.62 ± 0.038
3.325PheIle: 3.325 ± 0.115
3.102PheLys: 3.102 ± 0.092
3.781PheLeu: 3.781 ± 0.104
1.195PheMet: 1.195 ± 0.056
2.031PheAsn: 2.031 ± 0.073
1.265PhePro: 1.265 ± 0.053
1.374PheGln: 1.374 ± 0.05
1.19PheArg: 1.19 ± 0.058
2.673PheSer: 2.673 ± 0.104
2.398PheThr: 2.398 ± 0.084
2.958PheVal: 2.958 ± 0.089
0.474PheTrp: 0.474 ± 0.036
1.773PheTyr: 1.773 ± 0.07
0.002PheXaa: 0.002 ± 0.003
Gly
4.252GlyAla: 4.252 ± 0.111
0.513GlyCys: 0.513 ± 0.039
3.067GlyAsp: 3.067 ± 0.096
3.781GlyGlu: 3.781 ± 0.098
2.896GlyPhe: 2.896 ± 0.105
4.111GlyGly: 4.111 ± 0.138
1.364GlyHis: 1.364 ± 0.06
5.199GlyIle: 5.199 ± 0.131
4.572GlyLys: 4.572 ± 0.107
6.087GlyLeu: 6.087 ± 0.152
1.884GlyMet: 1.884 ± 0.068
2.46GlyAsn: 2.46 ± 0.082
1.446GlyPro: 1.446 ± 0.067
2.732GlyGln: 2.732 ± 0.081
2.504GlyArg: 2.504 ± 0.099
3.605GlySer: 3.605 ± 0.103
3.583GlyThr: 3.583 ± 0.088
4.671GlyVal: 4.671 ± 0.121
0.588GlyTrp: 0.588 ± 0.046
2.757GlyTyr: 2.757 ± 0.094
0.002GlyXaa: 0.002 ± 0.003
His
1.145HisAla: 1.145 ± 0.049
0.114HisCys: 0.114 ± 0.018
1.101HisAsp: 1.101 ± 0.059
1.093HisGlu: 1.093 ± 0.045
0.848HisPhe: 0.848 ± 0.036
1.26HisGly: 1.26 ± 0.056
0.575HisHis: 0.575 ± 0.049
1.16HisIle: 1.16 ± 0.051
1.2HisLys: 1.2 ± 0.052
2.001HisLeu: 2.001 ± 0.071
0.424HisMet: 0.424 ± 0.034
0.868HisAsn: 0.868 ± 0.051
0.974HisPro: 0.974 ± 0.046
1.002HisGln: 1.002 ± 0.056
0.679HisArg: 0.679 ± 0.043
0.92HisSer: 0.92 ± 0.045
0.883HisThr: 0.883 ± 0.047
1.227HisVal: 1.227 ± 0.052
0.183HisTrp: 0.183 ± 0.023
0.86HisTyr: 0.86 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
6.075IleAla: 6.075 ± 0.135
0.62IleCys: 0.62 ± 0.043
4.408IleAsp: 4.408 ± 0.107
4.778IleGlu: 4.778 ± 0.117
3.154IlePhe: 3.154 ± 0.091
4.78IleGly: 4.78 ± 0.134
1.178IleHis: 1.178 ± 0.055
5.936IleIle: 5.936 ± 0.171
5.678IleLys: 5.678 ± 0.142
7.039IleLeu: 7.039 ± 0.168
1.857IleMet: 1.857 ± 0.068
3.704IleAsn: 3.704 ± 0.101
3.134IlePro: 3.134 ± 0.096
2.946IleGln: 2.946 ± 0.091
2.43IleArg: 2.43 ± 0.085
4.461IleSer: 4.461 ± 0.112
4.763IleThr: 4.763 ± 0.122
5.326IleVal: 5.326 ± 0.109
0.677IleTrp: 0.677 ± 0.047
2.75IleTyr: 2.75 ± 0.101
0.002IleXaa: 0.002 ± 0.002
Lys
5.48LysAla: 5.48 ± 0.147
0.362LysCys: 0.362 ± 0.035
4.123LysAsp: 4.123 ± 0.125
5.913LysGlu: 5.913 ± 0.14
2.256LysPhe: 2.256 ± 0.069
3.791LysGly: 3.791 ± 0.103
1.265LysHis: 1.265 ± 0.059
6.03LysIle: 6.03 ± 0.136
6.665LysLys: 6.665 ± 0.164
6.531LysLeu: 6.531 ± 0.127
2.611LysMet: 2.611 ± 0.099
4.22LysAsn: 4.22 ± 0.116
2.328LysPro: 2.328 ± 0.094
3.667LysGln: 3.667 ± 0.131
3.149LysArg: 3.149 ± 0.09
3.278LysSer: 3.278 ± 0.079
4.597LysThr: 4.597 ± 0.106
5.162LysVal: 5.162 ± 0.114
0.655LysTrp: 0.655 ± 0.045
3.159LysTyr: 3.159 ± 0.107
0.0LysXaa: 0.0 ± 0.0
Leu
8.656LeuAla: 8.656 ± 0.182
0.62LeuCys: 0.62 ± 0.042
5.48LeuAsp: 5.48 ± 0.128
6.072LeuGlu: 6.072 ± 0.133
3.806LeuPhe: 3.806 ± 0.109
6.233LeuGly: 6.233 ± 0.146
1.641LeuHis: 1.641 ± 0.063
7.215LeuIle: 7.215 ± 0.169
7.228LeuLys: 7.228 ± 0.176
9.033LeuLeu: 9.033 ± 0.201
2.492LeuMet: 2.492 ± 0.084
5.053LeuAsn: 5.053 ± 0.109
3.987LeuPro: 3.987 ± 0.11
4.379LeuGln: 4.379 ± 0.15
3.466LeuArg: 3.466 ± 0.099
5.97LeuSer: 5.97 ± 0.134
6.578LeuThr: 6.578 ± 0.138
6.702LeuVal: 6.702 ± 0.113
0.739LeuTrp: 0.739 ± 0.048
3.013LeuTyr: 3.013 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
2.065MetAla: 2.065 ± 0.078
0.171MetCys: 0.171 ± 0.022
1.47MetAsp: 1.47 ± 0.062
1.659MetGlu: 1.659 ± 0.061
0.955MetPhe: 0.955 ± 0.053
1.641MetGly: 1.641 ± 0.073
0.474MetHis: 0.474 ± 0.034
2.031MetIle: 2.031 ± 0.075
2.127MetLys: 2.127 ± 0.067
2.554MetLeu: 2.554 ± 0.077
0.818MetMet: 0.818 ± 0.051
1.443MetAsn: 1.443 ± 0.059
1.111MetPro: 1.111 ± 0.053
1.473MetGln: 1.473 ± 0.066
1.017MetArg: 1.017 ± 0.052
1.478MetSer: 1.478 ± 0.062
1.574MetThr: 1.574 ± 0.068
1.676MetVal: 1.676 ± 0.072
0.171MetTrp: 0.171 ± 0.02
0.736MetTyr: 0.736 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.149AsnAla: 3.149 ± 0.087
0.332AsnCys: 0.332 ± 0.026
2.76AsnAsp: 2.76 ± 0.085
3.424AsnGlu: 3.424 ± 0.098
1.936AsnPhe: 1.936 ± 0.071
3.04AsnGly: 3.04 ± 0.109
1.056AsnHis: 1.056 ± 0.053
3.466AsnIle: 3.466 ± 0.092
3.702AsnLys: 3.702 ± 0.101
4.795AsnLeu: 4.795 ± 0.123
1.227AsnMet: 1.227 ± 0.057
2.715AsnAsn: 2.715 ± 0.116
2.06AsnPro: 2.06 ± 0.071
2.621AsnGln: 2.621 ± 0.096
1.703AsnArg: 1.703 ± 0.062
2.328AsnSer: 2.328 ± 0.075
2.231AsnThr: 2.231 ± 0.081
3.231AsnVal: 3.231 ± 0.087
0.536AsnTrp: 0.536 ± 0.034
2.234AsnTyr: 2.234 ± 0.087
0.002AsnXaa: 0.002 ± 0.002
Pro
2.75ProAla: 2.75 ± 0.091
0.206ProCys: 0.206 ± 0.024
2.033ProAsp: 2.033 ± 0.066
3.015ProGlu: 3.015 ± 0.104
1.488ProPhe: 1.488 ± 0.06
2.202ProGly: 2.202 ± 0.087
0.692ProHis: 0.692 ± 0.039
2.529ProIle: 2.529 ± 0.084
2.737ProLys: 2.737 ± 0.081
3.149ProLeu: 3.149 ± 0.087
0.687ProMet: 0.687 ± 0.042
1.808ProAsn: 1.808 ± 0.059
0.739ProPro: 0.739 ± 0.046
1.679ProGln: 1.679 ± 0.063
1.093ProArg: 1.093 ± 0.053
1.661ProSer: 1.661 ± 0.06
2.269ProThr: 2.269 ± 0.084
2.708ProVal: 2.708 ± 0.1
0.293ProTrp: 0.293 ± 0.029
1.47ProTyr: 1.47 ± 0.068
0.002ProXaa: 0.002 ± 0.003
Gln
4.951GlnAla: 4.951 ± 0.195
0.156GlnCys: 0.156 ± 0.019
2.353GlnAsp: 2.353 ± 0.074
3.506GlnGlu: 3.506 ± 0.129
1.465GlnPhe: 1.465 ± 0.063
2.792GlnGly: 2.792 ± 0.084
0.853GlnHis: 0.853 ± 0.046
3.595GlnIle: 3.595 ± 0.108
4.384GlnLys: 4.384 ± 0.134
4.173GlnLeu: 4.173 ± 0.124
1.274GlnMet: 1.274 ± 0.059
2.507GlnAsn: 2.507 ± 0.101
1.733GlnPro: 1.733 ± 0.076
3.03GlnGln: 3.03 ± 0.187
1.984GlnArg: 1.984 ± 0.073
2.07GlnSer: 2.07 ± 0.07
2.926GlnThr: 2.926 ± 0.105
3.526GlnVal: 3.526 ± 0.102
0.367GlnTrp: 0.367 ± 0.03
1.614GlnTyr: 1.614 ± 0.062
0.0GlnXaa: 0.0 ± 0.0
Arg
2.455ArgAla: 2.455 ± 0.079
0.268ArgCys: 0.268 ± 0.026
2.184ArgAsp: 2.184 ± 0.08
2.447ArgGlu: 2.447 ± 0.088
1.676ArgPhe: 1.676 ± 0.067
2.209ArgGly: 2.209 ± 0.084
0.722ArgHis: 0.722 ± 0.039
2.651ArgIle: 2.651 ± 0.082
2.943ArgLys: 2.943 ± 0.099
3.707ArgLeu: 3.707 ± 0.107
1.071ArgMet: 1.071 ± 0.049
1.892ArgAsn: 1.892 ± 0.065
1.297ArgPro: 1.297 ± 0.061
1.904ArgGln: 1.904 ± 0.08
2.001ArgArg: 2.001 ± 0.089
1.783ArgSer: 1.783 ± 0.064
1.847ArgThr: 1.847 ± 0.074
2.479ArgVal: 2.479 ± 0.102
0.293ArgTrp: 0.293 ± 0.028
1.696ArgTyr: 1.696 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.446SerAla: 3.446 ± 0.088
0.36SerCys: 0.36 ± 0.032
2.958SerAsp: 2.958 ± 0.082
3.498SerGlu: 3.498 ± 0.094
2.425SerPhe: 2.425 ± 0.089
3.756SerGly: 3.756 ± 0.081
1.014SerHis: 1.014 ± 0.05
3.585SerIle: 3.585 ± 0.124
3.303SerLys: 3.303 ± 0.1
5.489SerLeu: 5.489 ± 0.126
1.158SerMet: 1.158 ± 0.055
2.108SerAsn: 2.108 ± 0.074
1.681SerPro: 1.681 ± 0.068
2.767SerGln: 2.767 ± 0.097
2.013SerArg: 2.013 ± 0.076
2.96SerSer: 2.96 ± 0.113
2.579SerThr: 2.579 ± 0.091
3.533SerVal: 3.533 ± 0.108
0.563SerTrp: 0.563 ± 0.038
2.125SerTyr: 2.125 ± 0.09
0.0SerXaa: 0.0 ± 0.0
Thr
4.294ThrAla: 4.294 ± 0.1
0.325ThrCys: 0.325 ± 0.029
3.181ThrAsp: 3.181 ± 0.108
3.573ThrGlu: 3.573 ± 0.098
2.298ThrPhe: 2.298 ± 0.082
4.059ThrGly: 4.059 ± 0.113
1.041ThrHis: 1.041 ± 0.059
4.448ThrIle: 4.448 ± 0.114
4.074ThrLys: 4.074 ± 0.104
5.708ThrLeu: 5.708 ± 0.126
1.386ThrMet: 1.386 ± 0.054
2.67ThrAsn: 2.67 ± 0.095
2.653ThrPro: 2.653 ± 0.096
2.527ThrGln: 2.527 ± 0.1
1.803ThrArg: 1.803 ± 0.075
2.973ThrSer: 2.973 ± 0.087
3.863ThrThr: 3.863 ± 0.101
4.027ThrVal: 4.027 ± 0.103
0.508ThrTrp: 0.508 ± 0.036
2.115ThrTyr: 2.115 ± 0.056
0.005ThrXaa: 0.005 ± 0.003
Val
6.045ValAla: 6.045 ± 0.136
0.526ValCys: 0.526 ± 0.036
3.873ValAsp: 3.873 ± 0.105
4.076ValGlu: 4.076 ± 0.127
2.698ValPhe: 2.698 ± 0.088
4.456ValGly: 4.456 ± 0.111
1.076ValHis: 1.076 ± 0.055
5.715ValIle: 5.715 ± 0.147
5.142ValLys: 5.142 ± 0.142
6.905ValLeu: 6.905 ± 0.119
1.793ValMet: 1.793 ± 0.065
3.32ValAsn: 3.32 ± 0.084
2.544ValPro: 2.544 ± 0.071
2.893ValGln: 2.893 ± 0.092
2.355ValArg: 2.355 ± 0.093
3.94ValSer: 3.94 ± 0.102
4.423ValThr: 4.423 ± 0.122
5.549ValVal: 5.549 ± 0.126
0.538ValTrp: 0.538 ± 0.04
2.427ValTyr: 2.427 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.466TrpAla: 0.466 ± 0.033
0.072TrpCys: 0.072 ± 0.015
0.531TrpAsp: 0.531 ± 0.039
0.541TrpGlu: 0.541 ± 0.038
0.424TrpPhe: 0.424 ± 0.03
0.583TrpGly: 0.583 ± 0.041
0.216TrpHis: 0.216 ± 0.024
0.655TrpIle: 0.655 ± 0.038
0.528TrpLys: 0.528 ± 0.038
1.086TrpLeu: 1.086 ± 0.055
0.278TrpMet: 0.278 ± 0.023
0.456TrpAsn: 0.456 ± 0.035
0.188TrpPro: 0.188 ± 0.023
0.469TrpGln: 0.469 ± 0.039
0.382TrpArg: 0.382 ± 0.031
0.488TrpSer: 0.488 ± 0.037
0.374TrpThr: 0.374 ± 0.032
0.568TrpVal: 0.568 ± 0.04
0.087TrpTrp: 0.087 ± 0.014
0.352TrpTyr: 0.352 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.747TyrAla: 2.747 ± 0.084
0.295TyrCys: 0.295 ± 0.029
2.167TyrAsp: 2.167 ± 0.082
2.231TyrGlu: 2.231 ± 0.083
1.904TyrPhe: 1.904 ± 0.084
2.499TyrGly: 2.499 ± 0.085
0.89TyrHis: 0.89 ± 0.047
2.504TyrIle: 2.504 ± 0.088
1.949TyrLys: 1.949 ± 0.086
4.639TyrLeu: 4.639 ± 0.124
0.853TyrMet: 0.853 ± 0.044
1.693TyrAsn: 1.693 ± 0.075
1.599TyrPro: 1.599 ± 0.071
2.794TyrGln: 2.794 ± 0.106
1.845TyrArg: 1.845 ± 0.071
1.899TyrSer: 1.899 ± 0.08
2.031TyrThr: 2.031 ± 0.075
2.41TyrVal: 2.41 ± 0.09
0.347TyrTrp: 0.347 ± 0.026
1.912TyrTyr: 1.912 ± 0.086
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.002XaaLeu: 0.002 ± 0.002
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.007XaaGln: 0.007 ± 0.005
0.002XaaArg: 0.002 ± 0.003
0.005XaaSer: 0.005 ± 0.003
0.0XaaThr: 0.0 ± 0.0
0.002XaaVal: 0.002 ± 0.002
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.446XaaXaa: 0.446 ± 0.199
Statistics based on 1244 proteins (403319 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski