Clostridiales bacterium KA00134

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A137STR8|A0A137STR8_9FIRM Transporter major facilitator family protein OS=Clostridiales bacterium KA00134 OX=1588750 GN=HMPREF3189_01159 PE=4 SV=1
MM1 pKa = 7.84TYY3 pKa = 10.51QFNQFSQKK11 pKa = 10.55HH12 pKa = 5.19EE13 pKa = 4.66IIDD16 pKa = 3.63FEE18 pKa = 4.36IFLTSDD24 pKa = 3.58CEE26 pKa = 4.14YY27 pKa = 11.24DD28 pKa = 4.84DD29 pKa = 4.89IMLGRR34 pKa = 11.84IDD36 pKa = 3.73FFEE39 pKa = 4.44YY40 pKa = 10.0LWQDD44 pKa = 3.06EE45 pKa = 4.76VEE47 pKa = 4.39VKK49 pKa = 10.73KK50 pKa = 10.6FIASISFYY58 pKa = 10.6IFNIYY63 pKa = 10.19NIEE66 pKa = 4.2DD67 pKa = 3.79MLLLADD73 pKa = 5.08SISGDD78 pKa = 3.62LEE80 pKa = 4.54YY81 pKa = 11.03VASAYY86 pKa = 7.85NTYY89 pKa = 10.09CNEE92 pKa = 3.38VRR94 pKa = 11.84GIGSVAIIDD103 pKa = 4.09EE104 pKa = 4.55FNLADD109 pKa = 3.97EE110 pKa = 5.62AEE112 pKa = 4.3DD113 pKa = 4.3FYY115 pKa = 11.93GKK117 pKa = 9.68IQIIKK122 pKa = 8.61YY123 pKa = 9.01FLVKK127 pKa = 10.04TIEE130 pKa = 3.99KK131 pKa = 9.82LQIIGTGTLLFMSKK145 pKa = 10.88ALILDD150 pKa = 3.45INKK153 pKa = 9.66SDD155 pKa = 4.15RR156 pKa = 11.84IYY158 pKa = 10.86LINEE162 pKa = 4.14LLDD165 pKa = 3.59INCIPIYY172 pKa = 9.99QDD174 pKa = 2.93EE175 pKa = 4.68SDD177 pKa = 3.9VVLARR182 pKa = 11.84NLDD185 pKa = 3.71LTVV188 pKa = 3.05

Molecular weight: 21.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A137SW25|A0A137SW25_9FIRM S4 domain protein OS=Clostridiales bacterium KA00134 OX=1588750 GN=HMPREF3189_00655 PE=4 SV=1
MM1 pKa = 7.52VNIILVSFTKK11 pKa = 10.69GGINVKK17 pKa = 9.06RR18 pKa = 11.84TFQPKK23 pKa = 8.33NKK25 pKa = 9.37SRR27 pKa = 11.84KK28 pKa = 8.1RR29 pKa = 11.84EE30 pKa = 3.74HH31 pKa = 6.46GFRR34 pKa = 11.84ARR36 pKa = 11.84MRR38 pKa = 11.84TKK40 pKa = 10.48NGRR43 pKa = 11.84AVLKK47 pKa = 10.26ARR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 8.96GRR54 pKa = 11.84KK55 pKa = 8.89RR56 pKa = 11.84LSAA59 pKa = 3.96

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1672

0

1672

528957

37

4846

316.4

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.263 ± 0.058

0.917 ± 0.019

6.143 ± 0.054

7.629 ± 0.072

4.681 ± 0.055

6.511 ± 0.062

1.284 ± 0.021

8.781 ± 0.064

9.707 ± 0.07

9.585 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.033

5.228 ± 0.053

2.93 ± 0.04

2.513 ± 0.03

3.907 ± 0.041

6.32 ± 0.047

4.511 ± 0.063

5.941 ± 0.051

0.629 ± 0.017

3.973 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski