Acidobacteria bacterium SCGC AG-212-P17

Taxonomy: cellular organisms; Bacteria; Acidobacteria; unclassified Acidobacteria

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177RFP3|A0A177RFP3_9BACT Lon protease OS=Acidobacteria bacterium SCGC AG-212-P17 OX=1803033 GN=lon PE=2 SV=1
MM1 pKa = 7.43FFLLVASGILFLPKK15 pKa = 10.14FSLADD20 pKa = 3.42GVIIQTPAQGHH31 pKa = 4.91TVSITHH37 pKa = 6.06TVSSFDD43 pKa = 3.37IGTAAANVTIANTGMWISFNPGISQANFCGRR74 pKa = 11.84SDD76 pKa = 4.77FGVLDD81 pKa = 4.03INLHH85 pKa = 6.73DD86 pKa = 4.0NTTGQDD92 pKa = 3.24AYY94 pKa = 11.11SGQLAFDD101 pKa = 3.32QGVYY105 pKa = 9.16GTTGLSHH112 pKa = 6.72IHH114 pKa = 7.11DD115 pKa = 3.97VTAGDD120 pKa = 5.69DD121 pKa = 3.37IRR123 pKa = 11.84IDD125 pKa = 4.07LNCDD129 pKa = 2.99PSSATTTITAGITDD143 pKa = 4.05IPSVYY148 pKa = 7.92WWNGSGMTQPQYY160 pKa = 11.61DD161 pKa = 3.45AMIAYY166 pKa = 7.32MTSQLTVSSLHH177 pKa = 5.75QYY179 pKa = 10.59KK180 pKa = 10.4SDD182 pKa = 3.06ASTNIAEE189 pKa = 4.86GGLTTEE195 pKa = 4.04NSLVIKK201 pKa = 8.91GIPTVASGTDD211 pKa = 3.26QLRR214 pKa = 11.84LEE216 pKa = 5.21LEE218 pKa = 4.25VQPFATAFTGTATATSAATSSGNVVSLTIPSLANNHH254 pKa = 4.68YY255 pKa = 9.04HH256 pKa = 4.99WRR258 pKa = 11.84ARR260 pKa = 11.84VFDD263 pKa = 3.8TTTNATSSWEE273 pKa = 3.98EE274 pKa = 3.86FGTSGNADD282 pKa = 3.54FTIHH286 pKa = 7.06AGGNTADD293 pKa = 3.81LYY295 pKa = 11.57YY296 pKa = 10.15PVGYY300 pKa = 10.12QWISKK305 pKa = 8.36NAQYY309 pKa = 10.69CYY311 pKa = 11.0DD312 pKa = 3.96HH313 pKa = 7.17FSTSSPACSGVTSATSSDD331 pKa = 3.07NVYY334 pKa = 10.15WGGLVSDD341 pKa = 3.93NTAISFSGYY350 pKa = 10.42VSMLLYY356 pKa = 10.5DD357 pKa = 4.37ASSTSQCASGWWGHH371 pKa = 5.38TQDD374 pKa = 4.75IVDD377 pKa = 4.05ASGTLTDD384 pKa = 3.89VFDD387 pKa = 4.7GSVAAVGGVICHH399 pKa = 6.18NAGGAKK405 pKa = 9.5TISGPLTSRR414 pKa = 11.84RR415 pKa = 11.84YY416 pKa = 8.96VVVWQDD422 pKa = 3.55PAHH425 pKa = 6.36KK426 pKa = 10.29CADD429 pKa = 3.64EE430 pKa = 4.66SLSQCLADD438 pKa = 4.79HH439 pKa = 6.78GGDD442 pKa = 3.43SDD444 pKa = 4.96FSLTTLSFDD453 pKa = 3.91PVSPPPDD460 pKa = 3.43NARR463 pKa = 11.84SIYY466 pKa = 10.59LDD468 pKa = 3.49GSSALGYY475 pKa = 9.94SAANVAFTATDD486 pKa = 3.64PFTIEE491 pKa = 3.13LWYY494 pKa = 10.66RR495 pKa = 11.84SIAPSSTVMKK505 pKa = 10.67FIDD508 pKa = 3.56TRR510 pKa = 11.84SGQGKK515 pKa = 9.06GFFLEE520 pKa = 4.02KK521 pKa = 10.05HH522 pKa = 6.12ANDD525 pKa = 4.8GIEE528 pKa = 4.58FFVACDD534 pKa = 3.14SGEE537 pKa = 4.2VLFHH541 pKa = 6.67GAGALADD548 pKa = 4.7DD549 pKa = 4.68LTLNPAFDD557 pKa = 3.57KK558 pKa = 11.02HH559 pKa = 6.1GVWHH563 pKa = 6.55HH564 pKa = 5.05VAITKK569 pKa = 10.49DD570 pKa = 3.52SGTSWTAFKK579 pKa = 10.74LYY581 pKa = 10.41FDD583 pKa = 5.93GISQSISMDD592 pKa = 3.12TSNTLSGSCFNSTSSDD608 pKa = 3.88AIWFGQNQNPSSTPTFFTGNLDD630 pKa = 3.58EE631 pKa = 4.11VRR633 pKa = 11.84IWNVEE638 pKa = 3.8KK639 pKa = 11.01SVDD642 pKa = 4.02DD643 pKa = 4.09IKK645 pKa = 11.53AAMATEE651 pKa = 4.31ATSTAGLLALWQFNNTSTNIVTSDD675 pKa = 3.34VASGQDD681 pKa = 3.53GMPVFATSTPFGHH694 pKa = 6.78FLIDD698 pKa = 3.2QTVYY702 pKa = 8.35PTSVRR707 pKa = 11.84DD708 pKa = 3.81GQLLYY713 pKa = 10.72YY714 pKa = 10.7SSGTSYY720 pKa = 11.0GSEE723 pKa = 3.9MSDD726 pKa = 4.99AASTWNALGDD736 pKa = 3.83LVIASTTSALSANVVVSDD754 pKa = 3.98VNSNSSDD761 pKa = 3.96YY762 pKa = 10.92AWEE765 pKa = 4.59GAWTPYY771 pKa = 7.86PTSSLPSLRR780 pKa = 11.84FNHH783 pKa = 6.6YY784 pKa = 8.22YY785 pKa = 9.47TDD787 pKa = 3.33SRR789 pKa = 11.84SYY791 pKa = 11.45DD792 pKa = 3.69EE793 pKa = 4.56IMHH796 pKa = 5.96VATHH800 pKa = 6.06EE801 pKa = 4.34LGHH804 pKa = 7.1AIGLDD809 pKa = 3.49HH810 pKa = 7.12SFLGNIMNYY819 pKa = 9.86YY820 pKa = 8.76VTAQTFLGGQDD831 pKa = 2.99QKK833 pKa = 11.89DD834 pKa = 3.54YY835 pKa = 11.54FFINDD840 pKa = 3.15EE841 pKa = 4.32GLWGNN846 pKa = 3.97

Molecular weight: 90.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177RCU4|A0A177RCU4_9BACT Uncharacterized protein OS=Acidobacteria bacterium SCGC AG-212-P17 OX=1803033 GN=AYO50_01475 PE=4 SV=1
MM1 pKa = 7.39NNATCCMMTTSRR13 pKa = 11.84VHH15 pKa = 5.68TSAGKK20 pKa = 10.0AADD23 pKa = 3.6GKK25 pKa = 10.65ARR27 pKa = 11.84PSSAGRR33 pKa = 11.84RR34 pKa = 11.84GFAVGKK40 pKa = 9.92FSLPMLILALLPKK53 pKa = 10.54CPACFAAYY61 pKa = 9.65LALGTGISVSVTVASLFRR79 pKa = 11.84TLLIGICVTSLVWIFASAFRR99 pKa = 11.84SALMNRR105 pKa = 11.84LRR107 pKa = 11.84SDD109 pKa = 2.95SRR111 pKa = 11.84LYY113 pKa = 10.92

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

481

0

481

150889

59

3347

313.7

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.245 ± 0.137

0.956 ± 0.039

5.02 ± 0.07

5.664 ± 0.138

4.123 ± 0.069

7.538 ± 0.099

2.21 ± 0.05

5.242 ± 0.091

4.506 ± 0.111

9.846 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.056

3.585 ± 0.081

5.287 ± 0.109

4.124 ± 0.077

5.928 ± 0.097

6.512 ± 0.106

5.724 ± 0.113

7.269 ± 0.085

1.35 ± 0.038

2.602 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski