Penicillium coprophilum
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8993 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6UCI3|A0A1V6UCI3_9EURO Acetohydroxy-acid synthase small subunit OS=Penicillium coprophilum OX=36646 GN=PENCOP_c012G00357 PE=3 SV=1
MM1 pKa = 7.26 HH2 pKa = 6.55 TQKK5 pKa = 11.08 LLPLCGLLLASNIAIASKK23 pKa = 10.91 LDD25 pKa = 3.82 LDD27 pKa = 5.33 DD28 pKa = 5.08 VPTPCRR34 pKa = 11.84 PACSSVIRR42 pKa = 11.84 TAKK45 pKa = 10.47 SCDD48 pKa = 3.26 AKK50 pKa = 10.81 HH51 pKa = 6.04 EE52 pKa = 4.29 RR53 pKa = 11.84 DD54 pKa = 3.57 SAEE57 pKa = 3.92 IQCICDD63 pKa = 2.94 WDD65 pKa = 3.85 AAKK68 pKa = 8.92 TQIPLCSACITQYY81 pKa = 11.13 QSDD84 pKa = 3.51 KK85 pKa = 10.79 RR86 pKa = 11.84 NNNFTDD92 pKa = 3.83 GDD94 pKa = 3.82 RR95 pKa = 11.84 GDD97 pKa = 4.43 YY98 pKa = 11.08 DD99 pKa = 5.61 DD100 pKa = 6.83 DD101 pKa = 6.73 SDD103 pKa = 6.56 DD104 pKa = 6.05 DD105 pKa = 6.18 DD106 pKa = 6.57 DD107 pKa = 6.68 DD108 pKa = 6.15 NEE110 pKa = 4.55 ALDD113 pKa = 4.68 LVYY116 pKa = 10.76 SCSLTTTTYY125 pKa = 11.04 NSAAVTTVTGTSTSTATSNTATTTSSSSTSEE156 pKa = 4.18 TNSQGSTGSTSSGSGSSDD174 pKa = 3.12 SVGNSAGAASSATSIPDD191 pKa = 3.17 AAAGISVPGAASMAGVMGLMALAWLL216 pKa = 4.36
Molecular weight: 22.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.884
IPC_protein 3.91
Toseland 3.668
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.732
Grimsley 3.579
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.368
Thurlkill 3.745
EMBOSS 3.923
Sillero 4.05
Patrickios 1.138
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A0A1V6UUQ0|A0A1V6UUQ0_9EURO SGNH_hydro domain-containing protein OS=Penicillium coprophilum OX=36646 GN=PENCOP_c004G00999 PE=4 SV=1
MM1 pKa = 6.97 FCLRR5 pKa = 11.84 ARR7 pKa = 11.84 AVPSTFRR14 pKa = 11.84 ATTSAMRR21 pKa = 11.84 TPMNRR26 pKa = 11.84 FAKK29 pKa = 10.14 IPQQLSTPSTTLSSRR44 pKa = 11.84 SFSSLLSTPTRR55 pKa = 11.84 FQPSRR60 pKa = 11.84 TLGARR65 pKa = 11.84 LSSPITTATASAFSPLSSLRR85 pKa = 11.84 SQKK88 pKa = 10.68 APSQSRR94 pKa = 11.84 SFSASASLGVRR105 pKa = 11.84 RR106 pKa = 11.84 VTFRR110 pKa = 11.84 PSRR113 pKa = 11.84 RR114 pKa = 11.84 VQKK117 pKa = 10.3 RR118 pKa = 11.84 RR119 pKa = 11.84 HH120 pKa = 5.75 GYY122 pKa = 9.09 LARR125 pKa = 11.84 KK126 pKa = 9.08 KK127 pKa = 10.39 DD128 pKa = 3.6 RR129 pKa = 11.84 NGRR132 pKa = 11.84 KK133 pKa = 6.83 TLIRR137 pKa = 11.84 RR138 pKa = 11.84 TLKK141 pKa = 10.34 GRR143 pKa = 11.84 KK144 pKa = 7.8 EE145 pKa = 4.02 LSWW148 pKa = 4.66
Molecular weight: 16.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.921
IPC_protein 12.427
Toseland 12.588
ProMoST 13.086
Dawson 12.588
Bjellqvist 12.588
Wikipedia 13.056
Rodwell 12.237
Grimsley 12.632
Solomon 13.086
Lehninger 12.983
Nozaki 12.588
DTASelect 12.588
Thurlkill 12.588
EMBOSS 13.086
Sillero 12.588
Patrickios 11.945
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8993
0
8993
4534520
12
9592
504.2
55.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.391 ± 0.025
1.227 ± 0.009
5.642 ± 0.017
6.108 ± 0.027
3.817 ± 0.016
6.751 ± 0.02
2.432 ± 0.01
5.191 ± 0.017
4.687 ± 0.024
9.016 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.276 ± 0.01
3.756 ± 0.014
5.996 ± 0.026
4.05 ± 0.018
5.906 ± 0.021
8.423 ± 0.029
5.968 ± 0.018
6.127 ± 0.017
1.463 ± 0.01
2.774 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here