Novosphingobium sp. B1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1ZE00|A0A1W1ZE00_9SPHN Methyl-accepting chemotaxis protein OS=Novosphingobium sp. B1 OX=1938756 GN=SAMN06272759_10372 PE=4 SV=1
MM1 pKa = 7.79LDD3 pKa = 3.6SDD5 pKa = 4.14AQDD8 pKa = 3.34VCSLPDD14 pKa = 3.27QVANCGWPEE23 pKa = 3.77NCLISQDD30 pKa = 3.81GPLEE34 pKa = 3.61IDD36 pKa = 3.07IRR38 pKa = 11.84HH39 pKa = 6.43DD40 pKa = 4.05FIEE43 pKa = 5.7DD44 pKa = 3.39PAQINTVPVLICAMVVLALANAAWIVLRR72 pKa = 4.73

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2AGZ9|A0A1W2AGZ9_9SPHN 2 3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Novosphingobium sp. B1 OX=1938756 GN=gpmA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4444

0

4444

1394920

26

6659

313.9

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.012 ± 0.054

0.878 ± 0.013

5.761 ± 0.045

5.517 ± 0.04

3.577 ± 0.025

8.857 ± 0.078

1.983 ± 0.021

4.903 ± 0.025

3.211 ± 0.033

9.851 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.021

2.698 ± 0.026

5.214 ± 0.038

3.254 ± 0.021

7.149 ± 0.046

5.554 ± 0.039

5.253 ± 0.034

7.24 ± 0.028

1.464 ± 0.018

2.156 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski