Sewage-associated circular DNA virus-2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J457|A0A075J457_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-2 OX=1519391 PE=4 SV=1
MM1 pKa = 7.17TRR3 pKa = 11.84FAAKK7 pKa = 10.01QFFLTYY13 pKa = 9.44PRR15 pKa = 11.84CDD17 pKa = 4.02LDD19 pKa = 4.6LQLLLDD25 pKa = 4.44GLTTALAPRR34 pKa = 11.84LFRR37 pKa = 11.84HH38 pKa = 6.6KK39 pKa = 10.06IVQEE43 pKa = 3.82RR44 pKa = 11.84HH45 pKa = 5.17GDD47 pKa = 3.54EE48 pKa = 4.43GLHH51 pKa = 4.1VHH53 pKa = 7.28AIIVCAEE60 pKa = 4.56RR61 pKa = 11.84IDD63 pKa = 3.82TSNPRR68 pKa = 11.84FFDD71 pKa = 3.28VAGFHH76 pKa = 6.96PNIQTVRR83 pKa = 11.84NLRR86 pKa = 11.84QAYY89 pKa = 7.66TYY91 pKa = 10.83LDD93 pKa = 3.91KK94 pKa = 11.45EE95 pKa = 4.53PVQALCNLDD104 pKa = 3.53EE105 pKa = 6.0PIPKK109 pKa = 9.5MSWGEE114 pKa = 4.04LLEE117 pKa = 4.49KK118 pKa = 10.32ATDD121 pKa = 3.36AVEE124 pKa = 4.89FMNLMKK130 pKa = 10.37KK131 pKa = 8.26YY132 pKa = 10.24HH133 pKa = 6.1PRR135 pKa = 11.84DD136 pKa = 3.62YY137 pKa = 10.71ILSFTRR143 pKa = 11.84LLDD146 pKa = 3.53FAEE149 pKa = 4.36VHH151 pKa = 5.31FTEE154 pKa = 5.25PPPLYY159 pKa = 7.2EE160 pKa = 4.16TPEE163 pKa = 4.77GYY165 pKa = 10.5SFLTYY170 pKa = 10.01RR171 pKa = 11.84PKK173 pKa = 10.82SLVLIGPSRR182 pKa = 11.84TGKK185 pKa = 5.9TTWARR190 pKa = 11.84SLGRR194 pKa = 11.84HH195 pKa = 5.39VYY197 pKa = 9.08WNSLVNLDD205 pKa = 3.57VWSPLADD212 pKa = 3.6YY213 pKa = 10.78IIFDD217 pKa = 4.49DD218 pKa = 4.3VDD220 pKa = 3.33IDD222 pKa = 3.63FLPGYY227 pKa = 9.3KK228 pKa = 9.64CWLGAQKK235 pKa = 10.66EE236 pKa = 4.28FTVTDD241 pKa = 3.62KK242 pKa = 11.32YY243 pKa = 10.86KK244 pKa = 10.7KK245 pKa = 9.45KK246 pKa = 10.6RR247 pKa = 11.84KK248 pKa = 9.55IMWGKK253 pKa = 8.41PCIWLCIDD261 pKa = 4.98DD262 pKa = 5.22KK263 pKa = 11.82DD264 pKa = 4.05PLLSSKK270 pKa = 10.21VYY272 pKa = 10.56RR273 pKa = 11.84DD274 pKa = 4.22WISMNCVFVTVINKK288 pKa = 8.86MYY290 pKa = 10.87

Molecular weight: 33.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J457|A0A075J457_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-2 OX=1519391 PE=4 SV=1
MM1 pKa = 7.0GVKK4 pKa = 10.36RR5 pKa = 11.84KK6 pKa = 8.35RR7 pKa = 11.84TSSNIYY13 pKa = 8.17NTAISAYY20 pKa = 9.14GAGKK24 pKa = 9.88AAYY27 pKa = 9.09SAAKK31 pKa = 8.04EE32 pKa = 4.28TYY34 pKa = 10.08KK35 pKa = 10.71AGQYY39 pKa = 9.58AKK41 pKa = 10.38KK42 pKa = 10.25VYY44 pKa = 10.25KK45 pKa = 9.99KK46 pKa = 10.87VKK48 pKa = 9.4TAVQRR53 pKa = 11.84NWPQAKK59 pKa = 9.58GLRR62 pKa = 11.84LGHH65 pKa = 6.06RR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 9.67IVSPSYY74 pKa = 10.51QGMSRR79 pKa = 11.84IEE81 pKa = 3.88EE82 pKa = 4.4SKK84 pKa = 10.18SHH86 pKa = 6.35SKK88 pKa = 10.86RR89 pKa = 11.84KK90 pKa = 7.31MSPYY94 pKa = 9.89IRR96 pKa = 11.84TICKK100 pKa = 9.13TNRR103 pKa = 11.84PQLMKK108 pKa = 10.03IDD110 pKa = 5.06GIVKK114 pKa = 9.96DD115 pKa = 3.98QLTTKK120 pKa = 10.12PNRR123 pKa = 11.84QAVSTVITVGGRR135 pKa = 11.84TTYY138 pKa = 11.7ALIQNSISNQAAQAGITEE156 pKa = 4.33TDD158 pKa = 3.31IGGHH162 pKa = 5.73PANSNSNVNQTKK174 pKa = 10.45RR175 pKa = 11.84LIALYY180 pKa = 10.09EE181 pKa = 4.15SGNIQIANQTTVSCYY196 pKa = 8.82MDD198 pKa = 4.37LYY200 pKa = 10.54IIKK203 pKa = 9.95QIKK206 pKa = 10.07ACDD209 pKa = 3.94LDD211 pKa = 3.76SAAIITHH218 pKa = 6.76GGPNANVGVEE228 pKa = 4.19LNTTSVTAPHH238 pKa = 6.5GTPEE242 pKa = 4.75AIWNLGLYY250 pKa = 9.97DD251 pKa = 3.8EE252 pKa = 4.81QKK254 pKa = 10.54INVGGLVWMMHH265 pKa = 6.23SDD267 pKa = 3.53HH268 pKa = 7.01HH269 pKa = 5.89GHH271 pKa = 6.6PVDD274 pKa = 3.61PVTVNSSNGTLKK286 pKa = 10.42QSKK289 pKa = 9.78KK290 pKa = 10.66SRR292 pKa = 11.84LKK294 pKa = 10.85CPQAWFTIISFFINN308 pKa = 3.08

Molecular weight: 33.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

598

290

308

299.0

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.856 ± 1.156

1.839 ± 0.394

5.184 ± 1.649

3.512 ± 0.666

3.177 ± 1.606

5.853 ± 1.177

2.843 ± 0.058

7.023 ± 1.034

7.86 ± 0.661

8.027 ± 2.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.05

5.017 ± 1.313

5.017 ± 0.817

4.013 ± 1.098

5.184 ± 0.465

6.355 ± 1.521

7.191 ± 0.675

6.187 ± 0.013

2.007 ± 0.516

4.515 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski