Emilia yellow vein virus-associated DNA beta
Average proteome isoelectric point is 4.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3VIY0|C3VIY0_9VIRU C1 OS=Emilia yellow vein virus-associated DNA beta OX=640136 GN=C1 PE=4 SV=1
MM1 pKa = 7.59 QIMTITYY8 pKa = 10.12 KK9 pKa = 9.14 NTKK12 pKa = 8.05 GVKK15 pKa = 10.14 FIIDD19 pKa = 3.25 VRR21 pKa = 11.84 LCPRR25 pKa = 11.84 LNVQVTMISTKK36 pKa = 10.57 EE37 pKa = 4.04 PILSKK42 pKa = 10.16 ATYY45 pKa = 8.0 TLPYY49 pKa = 8.44 NHH51 pKa = 6.55 NEE53 pKa = 3.57 IEE55 pKa = 4.22 EE56 pKa = 4.36 PFDD59 pKa = 4.2 FNGTEE64 pKa = 3.68 EE65 pKa = 4.89 AIRR68 pKa = 11.84 NTIQVMVHH76 pKa = 7.19 DD77 pKa = 5.05 IPFNDD82 pKa = 4.11 IKK84 pKa = 11.23 SEE86 pKa = 4.72 DD87 pKa = 3.73 ILDD90 pKa = 4.59 SIDD93 pKa = 3.05 ITMMDD98 pKa = 3.48 RR99 pKa = 11.84 FVFIDD104 pKa = 4.21 LDD106 pKa = 3.65 TGGTCKK112 pKa = 9.56 TRR114 pKa = 11.84 CICTLL119 pKa = 3.32
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.819
IPC2_protein 4.914
IPC_protein 4.774
Toseland 4.635
ProMoST 4.914
Dawson 4.749
Bjellqvist 4.889
Wikipedia 4.647
Rodwell 4.635
Grimsley 4.546
Solomon 4.749
Lehninger 4.698
Nozaki 4.863
DTASelect 5.054
Thurlkill 4.647
EMBOSS 4.673
Sillero 4.914
Patrickios 3.897
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.895
Protein with the highest isoelectric point:
>tr|C3VIY0|C3VIY0_9VIRU C1 OS=Emilia yellow vein virus-associated DNA beta OX=640136 GN=C1 PE=4 SV=1
MM1 pKa = 7.59 QIMTITYY8 pKa = 10.12 KK9 pKa = 9.14 NTKK12 pKa = 8.05 GVKK15 pKa = 10.14 FIIDD19 pKa = 3.25 VRR21 pKa = 11.84 LCPRR25 pKa = 11.84 LNVQVTMISTKK36 pKa = 10.57 EE37 pKa = 4.04 PILSKK42 pKa = 10.16 ATYY45 pKa = 8.0 TLPYY49 pKa = 8.44 NHH51 pKa = 6.55 NEE53 pKa = 3.57 IEE55 pKa = 4.22 EE56 pKa = 4.36 PFDD59 pKa = 4.2 FNGTEE64 pKa = 3.68 EE65 pKa = 4.89 AIRR68 pKa = 11.84 NTIQVMVHH76 pKa = 7.19 DD77 pKa = 5.05 IPFNDD82 pKa = 4.11 IKK84 pKa = 11.23 SEE86 pKa = 4.72 DD87 pKa = 3.73 ILDD90 pKa = 4.59 SIDD93 pKa = 3.05 ITMMDD98 pKa = 3.48 RR99 pKa = 11.84 FVFIDD104 pKa = 4.21 LDD106 pKa = 3.65 TGGTCKK112 pKa = 9.56 TRR114 pKa = 11.84 CICTLL119 pKa = 3.32
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.819
IPC2_protein 4.914
IPC_protein 4.774
Toseland 4.635
ProMoST 4.914
Dawson 4.749
Bjellqvist 4.889
Wikipedia 4.647
Rodwell 4.635
Grimsley 4.546
Solomon 4.749
Lehninger 4.698
Nozaki 4.863
DTASelect 5.054
Thurlkill 4.647
EMBOSS 4.673
Sillero 4.914
Patrickios 3.897
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.895
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
119
119
119
119.0
13.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.681 ± 0.0
3.361 ± 0.0
8.403 ± 0.0
5.882 ± 0.0
5.042 ± 0.0
3.361 ± 0.0
1.681 ± 0.0
13.445 ± 0.0
5.882 ± 0.0
5.882 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.042 ± 0.0
5.882 ± 0.0
4.202 ± 0.0
2.521 ± 0.0
4.202 ± 0.0
3.361 ± 0.0
11.765 ± 0.0
5.882 ± 0.0
0.0 ± 0.0
2.521 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here