Mimosa yellow leaf curl virus satellite DNA beta
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5H1F4|A5H1F4_9VIRU C1 protein OS=Mimosa yellow leaf curl virus satellite DNA beta OX=447600 GN=C1 PE=4 SV=1
MM1 pKa = 7.28 HH2 pKa = 7.5 SKK4 pKa = 7.93 MTIQYY9 pKa = 9.7 RR10 pKa = 11.84 NKK12 pKa = 9.7 KK13 pKa = 8.62 GLRR16 pKa = 11.84 FMIDD20 pKa = 2.54 VRR22 pKa = 11.84 LQGEE26 pKa = 4.31 RR27 pKa = 11.84 FVMVKK32 pKa = 9.67 IRR34 pKa = 11.84 FSSTRR39 pKa = 11.84 APALIKK45 pKa = 10.4 RR46 pKa = 11.84 RR47 pKa = 11.84 FNIPYY52 pKa = 9.19 GHH54 pKa = 7.46 DD55 pKa = 4.0 GIIAPFDD62 pKa = 4.05 FNGLEE67 pKa = 4.12 TEE69 pKa = 4.43 IKK71 pKa = 11.07 DD72 pKa = 3.37 MMEE75 pKa = 4.17 ILFKK79 pKa = 10.84 DD80 pKa = 3.42 ASLEE84 pKa = 4.22 TFKK87 pKa = 10.95 PEE89 pKa = 4.74 EE90 pKa = 4.03 MVQTIDD96 pKa = 3.52 IIMMHH101 pKa = 6.82 EE102 pKa = 4.27 ANVMDD107 pKa = 4.19 INLDD111 pKa = 3.01 VDD113 pKa = 3.77 YY114 pKa = 11.33 DD115 pKa = 3.79 VANNVGAA122 pKa = 4.38
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.8
IPC2_protein 5.69
IPC_protein 5.677
Toseland 6.122
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.326
Solomon 5.881
Lehninger 5.868
Nozaki 6.122
DTASelect 6.313
Thurlkill 6.287
EMBOSS 6.262
Sillero 6.224
Patrickios 4.647
IPC_peptide 5.906
IPC2_peptide 6.211
IPC2.peptide.svr19 6.25
Protein with the highest isoelectric point:
>tr|A5H1F4|A5H1F4_9VIRU C1 protein OS=Mimosa yellow leaf curl virus satellite DNA beta OX=447600 GN=C1 PE=4 SV=1
MM1 pKa = 7.28 HH2 pKa = 7.5 SKK4 pKa = 7.93 MTIQYY9 pKa = 9.7 RR10 pKa = 11.84 NKK12 pKa = 9.7 KK13 pKa = 8.62 GLRR16 pKa = 11.84 FMIDD20 pKa = 2.54 VRR22 pKa = 11.84 LQGEE26 pKa = 4.31 RR27 pKa = 11.84 FVMVKK32 pKa = 9.67 IRR34 pKa = 11.84 FSSTRR39 pKa = 11.84 APALIKK45 pKa = 10.4 RR46 pKa = 11.84 RR47 pKa = 11.84 FNIPYY52 pKa = 9.19 GHH54 pKa = 7.46 DD55 pKa = 4.0 GIIAPFDD62 pKa = 4.05 FNGLEE67 pKa = 4.12 TEE69 pKa = 4.43 IKK71 pKa = 11.07 DD72 pKa = 3.37 MMEE75 pKa = 4.17 ILFKK79 pKa = 10.84 DD80 pKa = 3.42 ASLEE84 pKa = 4.22 TFKK87 pKa = 10.95 PEE89 pKa = 4.74 EE90 pKa = 4.03 MVQTIDD96 pKa = 3.52 IIMMHH101 pKa = 6.82 EE102 pKa = 4.27 ANVMDD107 pKa = 4.19 INLDD111 pKa = 3.01 VDD113 pKa = 3.77 YY114 pKa = 11.33 DD115 pKa = 3.79 VANNVGAA122 pKa = 4.38
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.8
IPC2_protein 5.69
IPC_protein 5.677
Toseland 6.122
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.326
Solomon 5.881
Lehninger 5.868
Nozaki 6.122
DTASelect 6.313
Thurlkill 6.287
EMBOSS 6.262
Sillero 6.224
Patrickios 4.647
IPC_peptide 5.906
IPC2_peptide 6.211
IPC2.peptide.svr19 6.25
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
122
122
122
122.0
14.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.738 ± 0.0
0.0 ± 0.0
8.197 ± 0.0
6.557 ± 0.0
6.557 ± 0.0
4.918 ± 0.0
2.459 ± 0.0
10.656 ± 0.0
6.557 ± 0.0
5.738 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
8.197 ± 0.0
5.738 ± 0.0
3.279 ± 0.0
2.459 ± 0.0
6.557 ± 0.0
3.279 ± 0.0
4.098 ± 0.0
6.557 ± 0.0
0.0 ± 0.0
2.459 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here