Sodalis-like endosymbiont of Proechinophthirus fluctus
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1269 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A151UM84|A0A151UM84_9GAMM Isoform of A0A151UM80 23S rRNA methyltransferase (Fragment) OS=Sodalis-like endosymbiont of Proechinophthirus fluctus OX=1462730 GN=BG74_00825 PE=4 SV=1
FF1 pKa = 6.4 YY2 pKa = 10.39 TPDD5 pKa = 3.42 PQMFDD10 pKa = 3.18 VLEE13 pKa = 4.47 KK14 pKa = 11.01 YY15 pKa = 10.57 GILGVEE21 pKa = 4.26 MEE23 pKa = 4.14 AAGIYY28 pKa = 9.99 GVAAEE33 pKa = 4.75 FGARR37 pKa = 11.84 ALAICTVSDD46 pKa = 4.5 HH47 pKa = 6.28 IRR49 pKa = 11.84 SGEE52 pKa = 3.95 ALSAADD58 pKa = 4.17 RR59 pKa = 11.84 QTTFNDD65 pKa = 3.71 MIEE68 pKa = 4.24 VALEE72 pKa = 4.04 SVLLGDD78 pKa = 4.0 PCC80 pKa = 6.29
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.824
IPC2_protein 4.075
IPC_protein 3.948
Toseland 3.77
ProMoST 4.126
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.834
Rodwell 3.783
Grimsley 3.694
Solomon 3.91
Lehninger 3.859
Nozaki 4.05
DTASelect 4.202
Thurlkill 3.821
EMBOSS 3.846
Sillero 4.062
Patrickios 1.914
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.966
Protein with the highest isoelectric point:
>tr|A0A151UKM3|A0A151UKM3_9GAMM Uncharacterized protein OS=Sodalis-like endosymbiont of Proechinophthirus fluctus OX=1462730 GN=BG74_04755 PE=4 SV=1
MM1 pKa = 7.73 IILSAPHH8 pKa = 4.91 TTKK11 pKa = 10.7 LAYY14 pKa = 8.34 ATGMGKK20 pKa = 9.52 VLVAANNWSTYY31 pKa = 10.41 SPATRR36 pKa = 11.84 RR37 pKa = 11.84 LEE39 pKa = 3.98 AGRR42 pKa = 11.84 LVVRR46 pKa = 11.84 RR47 pKa = 11.84 EE48 pKa = 3.74 NLAYY52 pKa = 9.29 PAAEE56 pKa = 4.4 TGSSAGPARR65 pKa = 11.84 RR66 pKa = 11.84 PLCRR70 pKa = 11.84 CFFLTAGCCLPPGGTTLKK88 pKa = 10.85 NQILTLGGDD97 pKa = 3.45 GHH99 pKa = 7.19 NIFTAKK105 pKa = 10.34 PILLGRR111 pKa = 11.84 RR112 pKa = 3.57
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.663
IPC_protein 10.438
Toseland 10.482
ProMoST 10.511
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.833
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.599
IPC_peptide 10.716
IPC2_peptide 9.721
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1168
101
1269
249220
12
1342
196.4
21.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.055 ± 0.069
1.24 ± 0.033
5.337 ± 0.058
5.543 ± 0.067
3.853 ± 0.053
7.204 ± 0.073
2.413 ± 0.042
6.344 ± 0.065
4.346 ± 0.064
10.761 ± 0.099
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.89 ± 0.039
3.653 ± 0.053
4.211 ± 0.048
4.297 ± 0.063
6.364 ± 0.071
5.922 ± 0.052
5.171 ± 0.045
7.264 ± 0.063
1.163 ± 0.027
2.893 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here