Streptococcus phage IPP24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SA33|A0A1S5SA33_9CAUD Rho_N domain-containing protein OS=Streptococcus phage IPP24 OX=1916164 GN=IPP24_00041 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.52RR3 pKa = 11.84GLFGTFDD10 pKa = 3.85YY11 pKa = 11.25DD12 pKa = 4.1CDD14 pKa = 3.82YY15 pKa = 10.95LQPPEE20 pKa = 4.11PTEE23 pKa = 4.05EE24 pKa = 4.21RR25 pKa = 11.84DD26 pKa = 3.43LVDD29 pKa = 4.42WIFSAGQWIYY39 pKa = 11.59VGDD42 pKa = 4.05CC43 pKa = 3.39

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SA81|A0A1S5SA81_9CAUD YopX domain-containing protein OS=Streptococcus phage IPP24 OX=1916164 GN=IPP24_00028 PE=4 SV=1
MM1 pKa = 7.49GGASVKK7 pKa = 9.91IDD9 pKa = 3.47LKK11 pKa = 10.88GVEE14 pKa = 4.82KK15 pKa = 10.55KK16 pKa = 10.79VSPQNFAKK24 pKa = 10.51GQLAIANQMLLDD36 pKa = 4.15MDD38 pKa = 4.37PFVPKK43 pKa = 10.44RR44 pKa = 11.84KK45 pKa = 10.01GILRR49 pKa = 11.84ASGHH53 pKa = 4.8VRR55 pKa = 11.84QDD57 pKa = 2.72AVVYY61 pKa = 6.11VTPYY65 pKa = 10.49VRR67 pKa = 11.84LLYY70 pKa = 10.21YY71 pKa = 10.17GKK73 pKa = 9.93KK74 pKa = 9.48RR75 pKa = 11.84KK76 pKa = 9.93GFFSEE81 pKa = 4.06KK82 pKa = 8.54QRR84 pKa = 11.84KK85 pKa = 8.15FFFANKK91 pKa = 9.44EE92 pKa = 3.94KK93 pKa = 10.8LLSQKK98 pKa = 7.49PTPGTGPRR106 pKa = 11.84WDD108 pKa = 3.86KK109 pKa = 10.85KK110 pKa = 11.03ASALYY115 pKa = 9.38SKK117 pKa = 10.65NWAEE121 pKa = 4.31VGAKK125 pKa = 10.44AMGVKK130 pKa = 10.02

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10800

40

1145

192.9

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.769 ± 0.747

0.648 ± 0.119

6.139 ± 0.333

8.046 ± 0.581

4.241 ± 0.247

6.741 ± 0.579

1.481 ± 0.211

7.37 ± 0.272

8.296 ± 0.529

8.074 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.183

5.222 ± 0.305

2.722 ± 0.245

4.343 ± 0.262

4.528 ± 0.345

6.519 ± 0.382

5.204 ± 0.247

6.269 ± 0.262

1.12 ± 0.136

3.667 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski