Streptococcus satellite phage Javan304

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJH8|A0A4D5ZJH8_9VIRU DNA-binding protein OS=Streptococcus satellite phage Javan304 OX=2558624 GN=JavanS304_0005 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 4.76EE3 pKa = 4.01HH4 pKa = 5.62QQAHH8 pKa = 6.72PEE10 pKa = 3.91YY11 pKa = 9.84EE12 pKa = 4.05RR13 pKa = 11.84YY14 pKa = 8.55NFKK17 pKa = 10.94YY18 pKa = 10.39IEE20 pKa = 4.96DD21 pKa = 3.85GDD23 pKa = 4.63AIGAIIDD30 pKa = 3.91YY31 pKa = 10.28NVEE34 pKa = 3.93EE35 pKa = 4.57SVLQAEE41 pKa = 4.5QDD43 pKa = 3.53NAA45 pKa = 3.77

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZK12|A0A4D5ZK12_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan304 OX=2558624 GN=JavanS304_0011 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.25ITEE5 pKa = 4.16YY6 pKa = 10.68KK7 pKa = 9.9KK8 pKa = 10.05KK9 pKa = 10.45NGNIVYY15 pKa = 10.07RR16 pKa = 11.84SSVYY20 pKa = 10.79LGVDD24 pKa = 2.7KK25 pKa = 10.61LTGKK29 pKa = 10.25KK30 pKa = 10.31ARR32 pKa = 11.84TTVTAKK38 pKa = 9.86TKK40 pKa = 10.0TGVKK44 pKa = 9.65IKK46 pKa = 10.65ARR48 pKa = 11.84EE49 pKa = 4.27AINAFASNGYY59 pKa = 6.03TVKK62 pKa = 10.73DD63 pKa = 3.41KK64 pKa = 9.74PTITTYY70 pKa = 11.08KK71 pKa = 10.18EE72 pKa = 4.03LVKK75 pKa = 10.72VWWDD79 pKa = 3.31SYY81 pKa = 11.64KK82 pKa = 10.2NTVKK86 pKa = 10.73PNTRR90 pKa = 11.84QSMDD94 pKa = 2.91GLVRR98 pKa = 11.84VHH100 pKa = 6.94LLPVFGDD107 pKa = 3.64YY108 pKa = 11.04KK109 pKa = 10.72LDD111 pKa = 3.67KK112 pKa = 9.79LTTPILQQQVNKK124 pKa = 9.66WADD127 pKa = 3.33KK128 pKa = 10.63ANKK131 pKa = 9.13GEE133 pKa = 4.15KK134 pKa = 10.18GAFANYY140 pKa = 10.46SLLHH144 pKa = 5.84NMNKK148 pKa = 10.25RR149 pKa = 11.84ILKK152 pKa = 9.6YY153 pKa = 10.08GVAIQVIQYY162 pKa = 10.08NPANDD167 pKa = 3.56VIVPRR172 pKa = 11.84KK173 pKa = 6.2QQKK176 pKa = 9.21EE177 pKa = 4.03KK178 pKa = 10.5STVKK182 pKa = 10.85YY183 pKa = 10.68LDD185 pKa = 3.51NKK187 pKa = 8.84EE188 pKa = 4.13LKK190 pKa = 10.45QFLNYY195 pKa = 10.31LDD197 pKa = 4.7TLDD200 pKa = 3.5QSNYY204 pKa = 9.49EE205 pKa = 3.97NLFDD209 pKa = 3.81VVLYY213 pKa = 8.25KK214 pKa = 10.5TLLATGCRR222 pKa = 11.84ISEE225 pKa = 4.07ALALEE230 pKa = 4.29WADD233 pKa = 2.97IDD235 pKa = 4.78LEE237 pKa = 4.4NGVISINKK245 pKa = 7.32TLNRR249 pKa = 11.84YY250 pKa = 7.65QEE252 pKa = 4.1INSPKK257 pKa = 10.13SSAGYY262 pKa = 9.92RR263 pKa = 11.84DD264 pKa = 3.32IPIDD268 pKa = 3.63RR269 pKa = 11.84TTLLLLKK276 pKa = 10.07QYY278 pKa = 11.16KK279 pKa = 9.59NRR281 pKa = 11.84QQIQSWKK288 pKa = 9.82LGRR291 pKa = 11.84TEE293 pKa = 3.97TVVFSVFTEE302 pKa = 4.95KK303 pKa = 10.78YY304 pKa = 10.36AYY306 pKa = 10.28ACNLRR311 pKa = 11.84KK312 pKa = 9.65RR313 pKa = 11.84LNKK316 pKa = 9.68HH317 pKa = 5.6FEE319 pKa = 4.07NAGVTNVSFHH329 pKa = 6.03GFRR332 pKa = 11.84HH333 pKa = 4.73THH335 pKa = 4.12TTMMLYY341 pKa = 10.55AQTSPKK347 pKa = 10.18DD348 pKa = 3.36VQYY351 pKa = 11.49RR352 pKa = 11.84LGHH355 pKa = 6.01SNLLVTEE362 pKa = 3.92NVYY365 pKa = 9.05WHH367 pKa = 6.7TNQEE371 pKa = 4.08NAKK374 pKa = 9.53KK375 pKa = 10.29AVSNYY380 pKa = 6.63EE381 pKa = 3.82TAINNLL387 pKa = 3.52

Molecular weight: 44.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

2863

37

505

159.1

18.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.693 ± 0.508

0.419 ± 0.162

5.938 ± 0.516

8.662 ± 0.828

4.436 ± 0.552

4.331 ± 0.482

1.711 ± 0.246

7.09 ± 0.54

10.059 ± 0.476

10.199 ± 0.771

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.234

6.427 ± 0.444

2.515 ± 0.535

4.471 ± 0.317

4.715 ± 0.239

5.589 ± 0.534

5.589 ± 0.529

4.995 ± 0.485

0.908 ± 0.203

4.261 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski