Bordetella phage vB_BbrS_PHB09

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RN13|A0A5B8RN13_9CAUD Uncharacterized protein OS=Bordetella phage vB_BbrS_PHB09 OX=2601609 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84FYY4 pKa = 11.54ARR6 pKa = 11.84IWPQSGMGGRR16 pKa = 11.84IMAPEE21 pKa = 4.3FVQRR25 pKa = 11.84SSADD29 pKa = 3.98DD30 pKa = 3.4IDD32 pKa = 4.16EE33 pKa = 4.67EE34 pKa = 4.12EE35 pKa = 5.28AEE37 pKa = 4.51ACAEE41 pKa = 4.29DD42 pKa = 4.5YY43 pKa = 10.87FHH45 pKa = 6.95NHH47 pKa = 7.21DD48 pKa = 3.98GFEE51 pKa = 4.65ANWPLTFAIFDD62 pKa = 3.9GFTGPEE68 pKa = 3.34IGRR71 pKa = 11.84WEE73 pKa = 3.99VALDD77 pKa = 3.5YY78 pKa = 11.06EE79 pKa = 4.63PSFNAAQLHH88 pKa = 6.43KK89 pKa = 10.46GGQVV93 pKa = 2.75

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RNA1|A0A5B8RNA1_9CAUD Minor tail protein OS=Bordetella phage vB_BbrS_PHB09 OX=2601609 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84AGTLNTPLTVLGPAPGRR19 pKa = 11.84DD20 pKa = 3.49NWGHH24 pKa = 5.02TAGPRR29 pKa = 11.84PILGRR34 pKa = 11.84IWANVGRR41 pKa = 11.84LSGKK45 pKa = 10.11AFIRR49 pKa = 11.84SGAATSHH56 pKa = 4.6VTASIRR62 pKa = 11.84VRR64 pKa = 11.84HH65 pKa = 5.73GAAKK69 pKa = 10.21QYY71 pKa = 10.88GLAAGMVLVSPSVRR85 pKa = 11.84YY86 pKa = 8.82EE87 pKa = 4.02VLTVLPDD94 pKa = 3.64DD95 pKa = 4.41AQRR98 pKa = 11.84QHH100 pKa = 6.92VDD102 pKa = 3.85LVCKK106 pKa = 9.48EE107 pKa = 4.2VPPDD111 pKa = 3.25AA112 pKa = 6.02

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13182

38

1200

223.4

24.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.156 ± 0.763

0.888 ± 0.136

5.91 ± 0.254

5.819 ± 0.24

2.89 ± 0.165

8.982 ± 0.259

1.805 ± 0.189

4.681 ± 0.242

3.884 ± 0.284

7.897 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.152

2.594 ± 0.179

4.84 ± 0.327

4.347 ± 0.281

7.518 ± 0.302

5.022 ± 0.279

5.014 ± 0.289

6.865 ± 0.362

1.912 ± 0.143

2.488 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski