Pseudomonas phage MP42

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus; unclassified Casadabanvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6PBD6|I6PBD6_9CAUD Uncharacterized protein OS=Pseudomonas phage MP42 OX=1161903 GN=PPMP42_53 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84LYY4 pKa = 10.51QFSRR8 pKa = 11.84GAIRR12 pKa = 11.84WSYY15 pKa = 10.82NSSDD19 pKa = 4.7RR20 pKa = 11.84DD21 pKa = 3.12ITYY24 pKa = 10.21QNQIFRR30 pKa = 11.84TVPGGITDD38 pKa = 3.54NGIICSGDD46 pKa = 3.43PQSDD50 pKa = 3.53QFVITAPADD59 pKa = 3.6LDD61 pKa = 3.68VALLYY66 pKa = 8.52KK67 pKa = 10.05TRR69 pKa = 11.84SPSGAIDD76 pKa = 3.52LVVYY80 pKa = 10.5DD81 pKa = 3.72MHH83 pKa = 8.34YY84 pKa = 11.14GDD86 pKa = 4.46TEE88 pKa = 4.2AAVSWVGQIGDD99 pKa = 4.0VDD101 pKa = 3.86WPTVDD106 pKa = 3.06SCRR109 pKa = 11.84ITCVSEE115 pKa = 4.47DD116 pKa = 3.9EE117 pKa = 6.08LMDD120 pKa = 3.76QPGLIDD126 pKa = 3.86TYY128 pKa = 11.23CRR130 pKa = 11.84TCTAVLGDD138 pKa = 4.13HH139 pKa = 6.26RR140 pKa = 11.84CKK142 pKa = 11.05VNLVPHH148 pKa = 6.99RR149 pKa = 11.84VTLTPQSVSDD159 pKa = 3.25WMISSGVVAGYY170 pKa = 11.1ADD172 pKa = 3.42GWFTAGYY179 pKa = 9.64VEE181 pKa = 4.2WQVDD185 pKa = 2.96GDD187 pKa = 3.87NYY189 pKa = 10.92DD190 pKa = 3.21RR191 pKa = 11.84RR192 pKa = 11.84HH193 pKa = 5.9IEE195 pKa = 3.63RR196 pKa = 11.84HH197 pKa = 5.59AGADD201 pKa = 3.65LYY203 pKa = 10.83ILGGTQGIPAGAQLRR218 pKa = 11.84VYY220 pKa = 9.53PGCDD224 pKa = 3.47FLAEE228 pKa = 4.02TCDD231 pKa = 3.61AKK233 pKa = 10.88FDD235 pKa = 3.8NLLNFRR241 pKa = 11.84GINKK245 pKa = 9.18LQGKK249 pKa = 9.31SPFDD253 pKa = 4.12GDD255 pKa = 4.13QVWW258 pKa = 3.01

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6P8E0|I6P8E0_9CAUD Phage uncharacterized protein OS=Pseudomonas phage MP42 OX=1161903 GN=PPMP42_28 PE=4 SV=1
MM1 pKa = 7.71ALQPRR6 pKa = 11.84GIRR9 pKa = 11.84NNNPGNIVWSARR21 pKa = 11.84NNWQGQLPHH30 pKa = 6.42NPKK33 pKa = 9.79VEE35 pKa = 3.88PRR37 pKa = 11.84FARR40 pKa = 11.84FDD42 pKa = 3.52TAHH45 pKa = 6.2NGIRR49 pKa = 11.84ALAKK53 pKa = 10.57LLLNYY58 pKa = 10.24RR59 pKa = 11.84KK60 pKa = 9.47VHH62 pKa = 5.43GLRR65 pKa = 11.84TVEE68 pKa = 3.93SLIARR73 pKa = 11.84WAPSNEE79 pKa = 3.72NDD81 pKa = 2.89TRR83 pKa = 11.84AYY85 pKa = 8.05ATAVARR91 pKa = 11.84AMGVPPQAGLHH102 pKa = 5.82MDD104 pKa = 3.53QDD106 pKa = 4.01TLAALVTAIIRR117 pKa = 11.84HH118 pKa = 5.57EE119 pKa = 4.47NGQQPYY125 pKa = 9.68SAEE128 pKa = 3.99QIAQAVRR135 pKa = 11.84EE136 pKa = 4.2VLL138 pKa = 3.49

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11147

34

1158

210.3

23.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.089 ± 0.749

0.861 ± 0.13

5.741 ± 0.256

6.1 ± 0.275

2.655 ± 0.211

7.581 ± 0.299

1.767 ± 0.185

4.261 ± 0.205

3.391 ± 0.234

9.85 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.156

2.96 ± 0.209

5.051 ± 0.348

4.799 ± 0.258

7.67 ± 0.377

5.948 ± 0.314

5.338 ± 0.248

6.414 ± 0.274

1.713 ± 0.124

2.629 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski