Levilinea saccharolytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Levilinea

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P6Y1Y8|A0A0P6Y1Y8_9CHLR Amidohydro-rel domain-containing protein OS=Levilinea saccharolytica OX=229921 GN=ADN01_17615 PE=4 SV=1
MM1 pKa = 7.12FAVSLLALIFFIGLAVDD18 pKa = 3.8AGAIYY23 pKa = 8.89VTYY26 pKa = 9.88GQLKK30 pKa = 9.07RR31 pKa = 11.84AVDD34 pKa = 3.75AASVAAANDD43 pKa = 3.58FKK45 pKa = 11.1KK46 pKa = 10.89GYY48 pKa = 8.43TRR50 pKa = 11.84DD51 pKa = 3.51QMEE54 pKa = 4.2DD55 pKa = 2.71SARR58 pKa = 11.84QVLIVHH64 pKa = 5.75QVDD67 pKa = 4.14LDD69 pKa = 4.38RR70 pKa = 11.84ITLNVYY76 pKa = 9.87ICDD79 pKa = 3.97NNGDD83 pKa = 3.92GARR86 pKa = 11.84DD87 pKa = 3.43ADD89 pKa = 4.08LQTNVPQFFARR100 pKa = 11.84CPDD103 pKa = 3.57TANGEE108 pKa = 4.38SPRR111 pKa = 11.84KK112 pKa = 9.16FVWVDD117 pKa = 3.06ASMKK121 pKa = 10.54APFYY125 pKa = 10.56FLSLLGFQSVDD136 pKa = 2.96LSTNAISEE144 pKa = 4.2AAPIDD149 pKa = 4.03LLIVLDD155 pKa = 4.04ISEE158 pKa = 4.43SMGVDD163 pKa = 3.27TPGYY167 pKa = 8.13TVNDD171 pKa = 4.24FNPANCNANTEE182 pKa = 4.51SYY184 pKa = 11.48DD185 pKa = 4.37DD186 pKa = 5.07DD187 pKa = 6.28DD188 pKa = 4.55NAATAAIPIVGNCAPLIQAKK208 pKa = 9.64KK209 pKa = 10.15AAIKK213 pKa = 10.69LVDD216 pKa = 3.86TLYY219 pKa = 11.06DD220 pKa = 3.94GYY222 pKa = 11.39DD223 pKa = 3.39SVSVVTFDD231 pKa = 3.17QVAYY235 pKa = 9.24QVPIADD241 pKa = 4.44TYY243 pKa = 12.24DD244 pKa = 3.19MAAVKK249 pKa = 9.93QAIGAIALHH258 pKa = 7.35DD259 pKa = 4.53DD260 pKa = 3.65PPYY263 pKa = 10.99NRR265 pKa = 11.84LWDD268 pKa = 3.35TWKK271 pKa = 10.96NPGRR275 pKa = 11.84VNLSNPEE282 pKa = 4.01DD283 pKa = 4.43RR284 pKa = 11.84DD285 pKa = 3.71GDD287 pKa = 3.86GADD290 pKa = 3.63YY291 pKa = 11.08DD292 pKa = 3.94NPAVVGYY299 pKa = 7.2TCPTFTANDD308 pKa = 3.89GTEE311 pKa = 4.01FGPPEE316 pKa = 4.05EE317 pKa = 5.45HH318 pKa = 7.18YY319 pKa = 11.31LDD321 pKa = 4.47DD322 pKa = 4.77RR323 pKa = 11.84WWQTGNPALVPGGEE337 pKa = 4.23GAPDD341 pKa = 3.65PFGWGGVPCDD351 pKa = 4.91RR352 pKa = 11.84DD353 pKa = 4.01DD354 pKa = 5.81KK355 pKa = 11.5LDD357 pKa = 3.9AYY359 pKa = 10.83DD360 pKa = 3.34WDD362 pKa = 5.16RR363 pKa = 11.84NGKK366 pKa = 5.66WTQYY370 pKa = 11.49DD371 pKa = 3.8HH372 pKa = 7.09DD373 pKa = 4.56TSGAWLTANDD383 pKa = 4.39PDD385 pKa = 4.16GAGALSVTLSPLSTCTGCGIRR406 pKa = 11.84VAANVLRR413 pKa = 11.84QTGRR417 pKa = 11.84PSSVWVMVLLSDD429 pKa = 4.49GAVNLSDD436 pKa = 3.2THH438 pKa = 7.63VNAGEE443 pKa = 4.07LNGEE447 pKa = 4.2DD448 pKa = 5.08VIPSWFPMGFCTGRR462 pKa = 11.84LFGPCVKK469 pKa = 10.55DD470 pKa = 3.83FGVGAEE476 pKa = 4.84DD477 pKa = 4.46GSANDD482 pKa = 5.49GYY484 pKa = 10.31WCSACQDD491 pKa = 3.3NFKK494 pKa = 9.97ATRR497 pKa = 11.84YY498 pKa = 9.93CVDD501 pKa = 3.97HH502 pKa = 7.22EE503 pKa = 4.47EE504 pKa = 4.27ATCPDD509 pKa = 3.69DD510 pKa = 3.78TTWIGDD516 pKa = 3.18TWTPNTLQKK525 pKa = 10.42RR526 pKa = 11.84YY527 pKa = 9.97SVYY530 pKa = 10.76DD531 pKa = 3.45YY532 pKa = 11.33ALDD535 pKa = 3.79MTDD538 pKa = 3.12EE539 pKa = 4.48AALTRR544 pKa = 11.84STKK547 pKa = 9.42LTEE550 pKa = 3.91PAGNDD555 pKa = 2.77IAIYY559 pKa = 9.65TIGLGAASSGADD571 pKa = 3.44LLRR574 pKa = 11.84YY575 pKa = 9.0LAAVGDD581 pKa = 4.36DD582 pKa = 3.92GDD584 pKa = 4.15RR585 pKa = 11.84TTDD588 pKa = 3.04PCAPFAANPTRR599 pKa = 11.84DD600 pKa = 3.1CGQYY604 pKa = 10.13YY605 pKa = 9.73FADD608 pKa = 3.44TSYY611 pKa = 10.71EE612 pKa = 3.89LQEE615 pKa = 4.07IFEE618 pKa = 5.66DD619 pKa = 3.39IASRR623 pKa = 11.84IYY625 pKa = 10.65TRR627 pKa = 11.84ITDD630 pKa = 3.36

Molecular weight: 68.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M9U2T0|A0A0M9U2T0_9CHLR ABC transporter permease OS=Levilinea saccharolytica OX=229921 GN=ADN01_16740 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 5.05PRR4 pKa = 11.84WYY6 pKa = 10.1PLILFVALLLLWGVLNAAFRR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 5.32RR29 pKa = 11.84QEE31 pKa = 3.87DD32 pKa = 4.18CPRR35 pKa = 11.84CGGPIEE41 pKa = 4.75RR42 pKa = 11.84IHH44 pKa = 7.06RR45 pKa = 11.84SKK47 pKa = 11.15LEE49 pKa = 3.84KK50 pKa = 10.69ALGLLLVYY58 pKa = 7.84PTGKK62 pKa = 9.63YY63 pKa = 9.21QCDD66 pKa = 3.08QCGWIGLRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84SHH78 pKa = 6.07RR79 pKa = 11.84RR80 pKa = 11.84STRR83 pKa = 11.84TT84 pKa = 3.07

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3080

0

3080

1055798

46

3580

342.8

37.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.801 ± 0.061

0.825 ± 0.012

4.801 ± 0.033

5.925 ± 0.052

3.908 ± 0.03

7.923 ± 0.044

1.885 ± 0.021

5.029 ± 0.037

2.997 ± 0.036

11.486 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.023

2.948 ± 0.024

5.546 ± 0.037

4.31 ± 0.031

6.354 ± 0.044

5.443 ± 0.031

5.335 ± 0.042

7.558 ± 0.044

1.691 ± 0.024

2.836 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski