Microdochium bolleyi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Microdochiaceae; Microdochium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A136IME4|A0A136IME4_9PEZI S-adenosylmethionine synthase OS=Microdochium bolleyi OX=196109 GN=Micbo1qcDRAFT_140634 PE=3 SV=1
MM1 pKa = 7.94PLNNHH6 pKa = 5.49NQRR9 pKa = 11.84LEE11 pKa = 4.19KK12 pKa = 10.1TMHH15 pKa = 6.19TISYY19 pKa = 9.94ASNNSYY25 pKa = 10.29FRR27 pKa = 11.84DD28 pKa = 3.71VQHH31 pKa = 7.35GSRR34 pKa = 11.84TEE36 pKa = 4.14SNTKK40 pKa = 9.77IAIEE44 pKa = 4.39TSTQAQPGSPADD56 pKa = 4.36HH57 pKa = 6.74ILVPLNTGDD66 pKa = 3.65QLLSNDD72 pKa = 3.44VATEE76 pKa = 3.99DD77 pKa = 3.88TMSPGAPEE85 pKa = 4.08HH86 pKa = 7.12CEE88 pKa = 3.84DD89 pKa = 4.04QAEE92 pKa = 4.26TEE94 pKa = 4.26PLSVHH99 pKa = 5.78TSIGLQTPGNAEE111 pKa = 3.71TSMLNTAQNPGQDD124 pKa = 3.82PPQDD128 pKa = 4.03PLQSPPQHH136 pKa = 6.58LAQNPGHH143 pKa = 6.42ASGYY147 pKa = 10.33ISDD150 pKa = 3.97TAQANARR157 pKa = 11.84PPEE160 pKa = 4.32PFQQATAQGEE170 pKa = 4.39INSNHH175 pKa = 6.95DD176 pKa = 3.15EE177 pKa = 4.61SALQTYY183 pKa = 8.01NQSQHH188 pKa = 6.16HH189 pKa = 5.63SQNTNHH195 pKa = 6.71PQQFSVRR202 pKa = 11.84QEE204 pKa = 4.02EE205 pKa = 4.68PGTSSAPAKK214 pKa = 10.25KK215 pKa = 10.14SSRR218 pKa = 11.84TDD220 pKa = 2.98NLLALGAGMVAGAAIGAAGYY240 pKa = 9.51AAYY243 pKa = 9.68KK244 pKa = 9.98KK245 pKa = 10.55LQGDD249 pKa = 3.92GEE251 pKa = 4.53GRR253 pKa = 11.84VQDD256 pKa = 5.47ARR258 pKa = 11.84DD259 pKa = 3.59SDD261 pKa = 4.07NDD263 pKa = 3.92SVLSWVSSISSRR275 pKa = 11.84SHH277 pKa = 6.81SSEE280 pKa = 3.97PNSVAGQGDD289 pKa = 4.11QMEE292 pKa = 4.84LDD294 pKa = 4.05YY295 pKa = 11.48EE296 pKa = 4.29QGTDD300 pKa = 3.26AQTKK304 pKa = 10.3SEE306 pKa = 4.58GFYY309 pKa = 10.87EE310 pKa = 4.39DD311 pKa = 5.64QFTSWDD317 pKa = 3.48QYY319 pKa = 11.63NEE321 pKa = 3.95YY322 pKa = 10.52QVFPEE327 pKa = 4.33EE328 pKa = 4.21VGSRR332 pKa = 11.84SYY334 pKa = 11.27GGPDD338 pKa = 3.44DD339 pKa = 5.19EE340 pKa = 5.64SWTSDD345 pKa = 3.5LPSDD349 pKa = 3.86AGSKK353 pKa = 10.17RR354 pKa = 11.84GISPAASLYY363 pKa = 10.89DD364 pKa = 3.6EE365 pKa = 4.36NEE367 pKa = 3.77YY368 pKa = 9.71WRR370 pKa = 11.84NSDD373 pKa = 3.78PQDD376 pKa = 3.86PDD378 pKa = 3.25VKK380 pKa = 10.72EE381 pKa = 4.28VMSGSAQHH389 pKa = 6.43SPISLSDD396 pKa = 3.52YY397 pKa = 10.1SAHH400 pKa = 6.57GLQSPGIVQPFDD412 pKa = 3.05WEE414 pKa = 4.55TNSDD418 pKa = 3.59QNGDD422 pKa = 3.26HH423 pKa = 7.12GDD425 pKa = 3.51PHH427 pKa = 8.22RR428 pKa = 11.84SVPPGSGEE436 pKa = 3.95DD437 pKa = 3.68HH438 pKa = 7.42DD439 pKa = 4.87VSDD442 pKa = 4.22WGQGDD447 pKa = 4.01GSVDD451 pKa = 3.69GSVHH455 pKa = 6.48GYY457 pKa = 9.91DD458 pKa = 5.03RR459 pKa = 11.84EE460 pKa = 4.22YY461 pKa = 10.69PDD463 pKa = 4.16YY464 pKa = 11.17DD465 pKa = 3.47PEE467 pKa = 4.24YY468 pKa = 10.38LHH470 pKa = 6.87SQFGDD475 pKa = 3.62EE476 pKa = 4.99LGSDD480 pKa = 3.75ADD482 pKa = 3.94SAAGALSEE490 pKa = 4.86VEE492 pKa = 3.89PSEE495 pKa = 4.3NGSALSGDD503 pKa = 4.24EE504 pKa = 4.83DD505 pKa = 5.01DD506 pKa = 6.6LMSQRR511 pKa = 11.84DD512 pKa = 3.56FSHH515 pKa = 7.32AYY517 pKa = 9.97EE518 pKa = 5.12SDD520 pKa = 3.88DD521 pKa = 4.68NPGDD525 pKa = 3.57EE526 pKa = 5.24DD527 pKa = 4.09GSYY530 pKa = 11.08SPGYY534 pKa = 10.68DD535 pKa = 3.5SDD537 pKa = 3.87AQSDD541 pKa = 3.93GFAGYY546 pKa = 10.56GSQRR550 pKa = 11.84DD551 pKa = 3.8EE552 pKa = 4.42YY553 pKa = 11.35GAGDD557 pKa = 5.12NIDD560 pKa = 5.16DD561 pKa = 5.36DD562 pKa = 5.2DD563 pKa = 6.16DD564 pKa = 5.62SGEE567 pKa = 4.21GEE569 pKa = 3.88QAYY572 pKa = 10.14EE573 pKa = 4.43SGGDD577 pKa = 3.89DD578 pKa = 4.11GGDD581 pKa = 3.79GNDD584 pKa = 3.44EE585 pKa = 4.46SQGEE589 pKa = 4.28AYY591 pKa = 10.13GYY593 pKa = 10.85DD594 pKa = 3.63GAQDD598 pKa = 3.69NPEE601 pKa = 3.98GAEE604 pKa = 4.05DD605 pKa = 3.7SGEE608 pKa = 4.18DD609 pKa = 3.79EE610 pKa = 5.31QIDD613 pKa = 4.22GPDD616 pKa = 3.51YY617 pKa = 11.11EE618 pKa = 5.09GNDD621 pKa = 3.95YY622 pKa = 11.63GDD624 pKa = 4.33ADD626 pKa = 4.68YY627 pKa = 11.75GDD629 pKa = 5.47DD630 pKa = 4.67DD631 pKa = 5.18DD632 pKa = 6.27GFYY635 pKa = 11.36EE636 pKa = 4.3EE637 pKa = 4.99

Molecular weight: 68.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A136IRZ4|A0A136IRZ4_9PEZI Uncharacterized protein (Fragment) OS=Microdochium bolleyi OX=196109 GN=Micbo1qcDRAFT_167258 PE=4 SV=1
MM1 pKa = 7.71ARR3 pKa = 11.84TTLLHH8 pKa = 6.24RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84IRR13 pKa = 11.84LLTLGAGPVAWRR25 pKa = 11.84WPASSVEE32 pKa = 3.86ARR34 pKa = 11.84RR35 pKa = 11.84GTRR38 pKa = 11.84RR39 pKa = 11.84QAGTRR44 pKa = 11.84RR45 pKa = 11.84GVRR48 pKa = 11.84AGRR51 pKa = 11.84PAGRR55 pKa = 11.84HH56 pKa = 3.79RR57 pKa = 11.84HH58 pKa = 4.87RR59 pKa = 11.84SARR62 pKa = 11.84FAQGPGSPAGGKK74 pKa = 7.41QVV76 pKa = 3.01

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13164

0

13164

5816858

50

11096

441.9

48.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.748 ± 0.022

1.32 ± 0.009

5.777 ± 0.017

5.687 ± 0.019

3.557 ± 0.013

7.374 ± 0.02

2.482 ± 0.011

4.485 ± 0.017

4.373 ± 0.021

8.777 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.007

3.318 ± 0.012

6.013 ± 0.019

4.008 ± 0.016

6.29 ± 0.018

8.048 ± 0.023

6.079 ± 0.021

6.304 ± 0.017

1.509 ± 0.008

2.627 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski