Methyloceanibacter methanicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3W3J0|A0A1E3W3J0_9RHIZ Uncharacterized protein OS=Methyloceanibacter methanicus OX=1774968 GN=AUC68_01010 PE=4 SV=1
MM1 pKa = 7.54IASCPDD7 pKa = 2.8GRR9 pKa = 11.84SAGTCLALLASAMQFAALALPGGAACAKK37 pKa = 10.11DD38 pKa = 4.36DD39 pKa = 4.17NAALAQQLANPIADD53 pKa = 3.64LTVVPFQYY61 pKa = 11.15DD62 pKa = 3.48LLLNGGPDD70 pKa = 3.65GDD72 pKa = 3.93ATGSVLTIQPIIPIHH87 pKa = 6.74LNKK90 pKa = 10.27DD91 pKa = 2.79WNLITRR97 pKa = 11.84TILPVAYY104 pKa = 10.04ASGIPTGDD112 pKa = 3.09FGGLGDD118 pKa = 3.97TQFSAFLSPQKK129 pKa = 10.63SGADD133 pKa = 3.43GLVWGIGSIVNLPTATNSDD152 pKa = 3.77LGTSEE157 pKa = 3.87WGAGPTAVALVQKK170 pKa = 10.21GPWTVGALANHH181 pKa = 7.28LWTFDD186 pKa = 3.82DD187 pKa = 4.82APAGAATRR195 pKa = 11.84SLTTTGAGYY204 pKa = 10.83VDD206 pKa = 3.66QTFTQPWVTYY216 pKa = 9.29GWGSGQSVTLTSQTSYY232 pKa = 10.63NWNSDD237 pKa = 2.91EE238 pKa = 4.0WTVPLNLTYY247 pKa = 10.77QSVGKK252 pKa = 10.6LGDD255 pKa = 3.25QSVQFQVGGAYY266 pKa = 10.28APIVPDD272 pKa = 5.6DD273 pKa = 3.76YY274 pKa = 11.6PNWGLQASLIFLFPNN289 pKa = 3.73

Molecular weight: 30.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3VZB5|A0A1E3VZB5_9RHIZ MoCF_biosynth domain-containing protein OS=Methyloceanibacter methanicus OX=1774968 GN=AUC68_06610 PE=4 SV=1
MM1 pKa = 7.46AVAKK5 pKa = 10.45KK6 pKa = 8.96KK7 pKa = 8.77TATKK11 pKa = 10.2KK12 pKa = 9.94KK13 pKa = 10.3APAKK17 pKa = 9.98RR18 pKa = 11.84KK19 pKa = 7.46TVAKK23 pKa = 9.82KK24 pKa = 9.1KK25 pKa = 8.58TVAKK29 pKa = 10.06KK30 pKa = 9.85RR31 pKa = 11.84APAKK35 pKa = 10.01RR36 pKa = 11.84KK37 pKa = 6.74TVRR40 pKa = 11.84KK41 pKa = 5.27TTAAKK46 pKa = 10.06KK47 pKa = 9.96RR48 pKa = 11.84VVKK51 pKa = 10.52KK52 pKa = 10.21RR53 pKa = 11.84APAKK57 pKa = 10.39KK58 pKa = 9.7RR59 pKa = 11.84VAKK62 pKa = 10.39KK63 pKa = 9.9KK64 pKa = 10.69APAKK68 pKa = 10.02KK69 pKa = 8.59KK70 pKa = 7.54TVRR73 pKa = 11.84KK74 pKa = 7.16TAAAKK79 pKa = 10.12KK80 pKa = 8.75RR81 pKa = 11.84TVKK84 pKa = 10.5KK85 pKa = 10.24SAPAKK90 pKa = 10.27KK91 pKa = 9.74RR92 pKa = 11.84VAKK95 pKa = 10.28KK96 pKa = 9.07KK97 pKa = 8.44TVAKK101 pKa = 10.29KK102 pKa = 9.68RR103 pKa = 11.84AKK105 pKa = 9.07KK106 pKa = 7.9TAA108 pKa = 3.25

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2710

0

2710

768685

29

2157

283.6

30.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.242 ± 0.073

0.936 ± 0.017

5.995 ± 0.035

6.095 ± 0.044

3.676 ± 0.032

8.517 ± 0.048

2.02 ± 0.023

4.841 ± 0.034

3.697 ± 0.037

10.004 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.021

2.518 ± 0.027

5.365 ± 0.035

2.999 ± 0.027

6.937 ± 0.047

5.278 ± 0.035

5.406 ± 0.034

7.485 ± 0.043

1.307 ± 0.018

2.346 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski