Wood mouse herpesvirus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D0U1P1|D0U1P1_9GAMA Virion protein G52 OS=Wood mouse herpesvirus OX=432370 GN=ORF52 PE=1 SV=1
MM1 pKa = 7.57 CGVKK5 pKa = 10.28 PIVKK9 pKa = 9.85 CFLLFHH15 pKa = 7.34 IINFLGTYY23 pKa = 9.08 NVGWVPGTPLCAAQAVDD40 pKa = 5.09 GITSRR45 pKa = 11.84 EE46 pKa = 3.86 MEE48 pKa = 4.35 INATLAPSSGATLSLLVTLSNNNPPTAMRR77 pKa = 11.84 PPVAQNGEE85 pKa = 4.4 SVSIDD90 pKa = 3.44 NSSAPASDD98 pKa = 3.58 PTTSNPSSPEE108 pKa = 3.89 EE109 pKa = 4.34 APTAAPITPIPTSTAAQSAEE129 pKa = 4.16 HH130 pKa = 6.57 VGEE133 pKa = 4.3 TDD135 pKa = 3.67 SEE137 pKa = 4.7 APTPLPTTPKK147 pKa = 10.05 PSSQEE152 pKa = 4.07 DD153 pKa = 4.65 EE154 pKa = 4.26 PTMTSPTEE162 pKa = 4.1 TPPTTTAAISTEE174 pKa = 3.96 QDD176 pKa = 3.37 DD177 pKa = 4.15 EE178 pKa = 4.67 TEE180 pKa = 4.59 PEE182 pKa = 4.52 SPAPPPATPEE192 pKa = 4.3 PEE194 pKa = 4.13 TTTPTKK200 pKa = 10.55 NQEE203 pKa = 4.1 DD204 pKa = 4.74 EE205 pKa = 4.28 PTINTSDD212 pKa = 3.43 QGDD215 pKa = 4.09 DD216 pKa = 3.54 SSSDD220 pKa = 3.34 IPAGTPGPTTPPKK233 pKa = 10.56 QEE235 pKa = 4.03 TEE237 pKa = 3.93 TTKK240 pKa = 10.82 PVDD243 pKa = 3.5 SKK245 pKa = 11.4 PQVEE249 pKa = 4.78 PNDD252 pKa = 4.07 SAPSDD257 pKa = 3.54 IPEE260 pKa = 4.6 TSDD263 pKa = 3.31 STPTPVTDD271 pKa = 3.57 PTSPPSVEE279 pKa = 4.07 EE280 pKa = 3.94 TSPAEE285 pKa = 4.02 PSTPDD290 pKa = 3.23 STPPADD296 pKa = 4.22 PPAPQPTPPAEE307 pKa = 4.41 PSTPDD312 pKa = 3.32 STPQDD317 pKa = 3.73 EE318 pKa = 4.92 PSTPDD323 pKa = 3.14 STPPADD329 pKa = 4.22 PPAPQPTPPAEE340 pKa = 4.36 PSTPEE345 pKa = 3.86 STPPADD351 pKa = 4.1 PPAAQPTPQAEE362 pKa = 4.3 PSTPDD367 pKa = 2.98 STSPSDD373 pKa = 3.85 PPASQPTLPEE383 pKa = 4.52 KK384 pKa = 10.49 PPSPEE389 pKa = 3.55 PMTPQSGPAITPEE402 pKa = 3.62 IATPSTTEE410 pKa = 3.65 PGAGKK415 pKa = 9.61 DD416 pKa = 3.31 ASMGATTTQAASALTTKK433 pKa = 10.24 PMRR436 pKa = 11.84 VVPDD440 pKa = 3.58 VSTMLWIRR448 pKa = 11.84 PTVAIVLIFLLMSIFHH464 pKa = 6.88 IMYY467 pKa = 8.91 CVCLHH472 pKa = 6.12 EE473 pKa = 4.44
Molecular weight: 48.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.169
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.528
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.617
Sillero 3.846
Patrickios 3.35
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|D0U1L1|D0U1L1_9GAMA Thymidine kinase OS=Wood mouse herpesvirus OX=432370 GN=ORF21 PE=3 SV=1
MM1 pKa = 7.94 DD2 pKa = 4.34 EE3 pKa = 4.88 KK4 pKa = 11.09 GAKK7 pKa = 9.11 QLLNKK12 pKa = 9.92 LPKK15 pKa = 9.66 RR16 pKa = 11.84 RR17 pKa = 11.84 ARR19 pKa = 11.84 AGKK22 pKa = 8.89 LAHH25 pKa = 5.4 VRR27 pKa = 11.84 CYY29 pKa = 10.57 RR30 pKa = 11.84 AMQGASNILQLLEE43 pKa = 4.08 SLKK46 pKa = 10.16 IAHH49 pKa = 6.52 QITHH53 pKa = 6.65 PVGSRR58 pKa = 11.84 LFFEE62 pKa = 4.5 VTLGRR67 pKa = 11.84 RR68 pKa = 11.84 VVDD71 pKa = 4.63 AIIVVFSEE79 pKa = 4.29 SSPHH83 pKa = 4.79 IHH85 pKa = 6.93 CFLIEE90 pKa = 4.49 LKK92 pKa = 9.19 TCKK95 pKa = 10.06 INFFNHH101 pKa = 5.93 NSTTRR106 pKa = 11.84 EE107 pKa = 3.83 AQRR110 pKa = 11.84 VEE112 pKa = 4.25 GSSQLRR118 pKa = 11.84 DD119 pKa = 3.24 SARR122 pKa = 11.84 ALTLLAPVGTDD133 pKa = 3.15 PCRR136 pKa = 11.84 VTAHH140 pKa = 7.4 LIFKK144 pKa = 7.91 SQRR147 pKa = 11.84 GLHH150 pKa = 5.06 TLKK153 pKa = 10.72 SYY155 pKa = 9.43 TLTWMTHH162 pKa = 5.79 TINTQKK168 pKa = 10.68 VALLNFLGLNADD180 pKa = 3.77 NEE182 pKa = 4.23 LRR184 pKa = 11.84 ACLTKK189 pKa = 10.59 GLPPARR195 pKa = 11.84 SLGRR199 pKa = 11.84 RR200 pKa = 11.84 SHH202 pKa = 6.01 VCLLEE207 pKa = 4.24 PKK209 pKa = 9.12 PQKK212 pKa = 9.84 HH213 pKa = 5.55 LKK215 pKa = 8.78 NRR217 pKa = 11.84 RR218 pKa = 11.84 GGASRR223 pKa = 11.84 NQKK226 pKa = 9.72 ARR228 pKa = 11.84 RR229 pKa = 11.84 QGVGPKK235 pKa = 9.8 VSHH238 pKa = 7.42 DD239 pKa = 3.45 KK240 pKa = 10.59 TGNAPAHH247 pKa = 5.52 AAGRR251 pKa = 11.84 AGG253 pKa = 3.44
Molecular weight: 28.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.765
IPC_protein 10.701
Toseland 11.155
ProMoST 11.111
Dawson 11.184
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.374
Grimsley 11.213
Solomon 11.447
Lehninger 11.389
Nozaki 11.14
DTASelect 10.965
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.14
Patrickios 11.096
IPC_peptide 11.447
IPC2_peptide 10.218
IPC2.peptide.svr19 8.884
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
2
74
35050
75
2455
473.6
53.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.886 ± 0.178
2.636 ± 0.175
4.805 ± 0.144
5.193 ± 0.138
4.514 ± 0.168
5.101 ± 0.207
2.862 ± 0.112
6.117 ± 0.179
5.187 ± 0.228
10.439 ± 0.268
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.491 ± 0.119
4.362 ± 0.119
6.191 ± 0.338
3.74 ± 0.12
4.217 ± 0.179
8.18 ± 0.201
7.384 ± 0.253
6.163 ± 0.204
1.096 ± 0.066
3.438 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here