Actinokineospora iranica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinokineospora

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6VXP1|A0A1G6VXP1_9PSEU Uncharacterized protein OS=Actinokineospora iranica OX=1271860 GN=SAMN05216174_113126 PE=4 SV=1
MM1 pKa = 8.14RR2 pKa = 11.84SRR4 pKa = 11.84ALYY7 pKa = 10.06SVLFAGTVVVGGLATGGSASAAANCVIGPGVTQTNTTVTGSGANDD52 pKa = 4.12TIDD55 pKa = 4.23CTNSDD60 pKa = 4.15PGKK63 pKa = 9.04TIYY66 pKa = 11.13GNGGNDD72 pKa = 4.15TITGTAFNDD81 pKa = 3.93VIHH84 pKa = 6.97GNDD87 pKa = 3.99GNDD90 pKa = 3.34TLTGGIGNDD99 pKa = 3.63TLSGGLGEE107 pKa = 4.38DD108 pKa = 3.94TVNGSAGNDD117 pKa = 3.46SLTGPGTDD125 pKa = 3.67FAVDD129 pKa = 3.69TLSGGTGTDD138 pKa = 2.66KK139 pKa = 11.02CGAIGIPPDD148 pKa = 3.36IRR150 pKa = 11.84SSCEE154 pKa = 3.59SS155 pKa = 3.2

Molecular weight: 14.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6QI66|A0A1G6QI66_9PSEU Perosamine synthetase OS=Actinokineospora iranica OX=1271860 GN=SAMN05216174_105314 PE=3 SV=1
MM1 pKa = 7.63AWRR4 pKa = 11.84GRR6 pKa = 11.84LPVGLRR12 pKa = 11.84LSLGLWLSLGLRR24 pKa = 11.84LSLGLLSLRR33 pKa = 11.84LGLPLGRR40 pKa = 11.84GLARR44 pKa = 11.84WVRR47 pKa = 11.84LAGLPLRR54 pKa = 11.84VRR56 pKa = 11.84PAA58 pKa = 3.01

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6124

0

6124

1928892

29

6588

315.0

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.938 ± 0.046

0.805 ± 0.011

6.252 ± 0.023

5.382 ± 0.028

2.782 ± 0.016

9.064 ± 0.035

2.28 ± 0.017

3.273 ± 0.022

2.009 ± 0.023

10.315 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.663 ± 0.012

1.835 ± 0.016

6.169 ± 0.037

2.632 ± 0.016

8.165 ± 0.032

4.914 ± 0.021

6.047 ± 0.028

9.097 ± 0.03

1.512 ± 0.013

1.864 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski