Nerine virus X
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2V0S0|Q2V0S0_9VIRU ORF1 protein OS=Nerine virus X OX=333348 PE=4 SV=1
MM1 pKa = 7.5 EE2 pKa = 5.7 NIINKK7 pKa = 8.91 LQTRR11 pKa = 11.84 FTRR14 pKa = 11.84 TSLPISKK21 pKa = 9.66 PIIIHH26 pKa = 5.93 CCAGAGKK33 pKa = 7.33 TTFIRR38 pKa = 11.84 EE39 pKa = 4.2 LLEE42 pKa = 3.95 QFPEE46 pKa = 4.35 VQAYY50 pKa = 6.81 THH52 pKa = 5.73 SQHH55 pKa = 6.61 LAAEE59 pKa = 4.35 KK60 pKa = 9.88 TISGRR65 pKa = 11.84 NFHH68 pKa = 6.82 HH69 pKa = 6.9 FSEE72 pKa = 4.55 YY73 pKa = 9.67 QPGYY77 pKa = 10.42 LDD79 pKa = 5.39 VIDD82 pKa = 5.35 EE83 pKa = 4.35 YY84 pKa = 11.62 LAGPIPEE91 pKa = 4.28 TCHH94 pKa = 6.21 FCFADD99 pKa = 4.76 PYY101 pKa = 10.75 QYY103 pKa = 11.3 NIDD106 pKa = 3.98 ALPAHH111 pKa = 6.96 FICNKK116 pKa = 9.37 SYY118 pKa = 11.23 RR119 pKa = 11.84 FGTLTADD126 pKa = 3.67 YY127 pKa = 9.97 LNSLGYY133 pKa = 8.14 QVRR136 pKa = 11.84 SAGGSDD142 pKa = 3.77 SIRR145 pKa = 11.84 FIDD148 pKa = 3.88 LAHH151 pKa = 7.06 WEE153 pKa = 4.3 PEE155 pKa = 4.83 GICITQDD162 pKa = 3.06 SDD164 pKa = 3.85 ILNLLTRR171 pKa = 11.84 HH172 pKa = 6.65 GISAYY177 pKa = 10.19 HH178 pKa = 6.1 PCEE181 pKa = 3.81 TLGLQFNCVDD191 pKa = 3.61 YY192 pKa = 10.86 LVPKK196 pKa = 10.55 LPTPATADD204 pKa = 3.34 ANTYY208 pKa = 10.17 LALTRR213 pKa = 11.84 HH214 pKa = 5.53 SSRR217 pKa = 11.84 LNVVSDD223 pKa = 3.93 AADD226 pKa = 3.31 SATT229 pKa = 3.56
Molecular weight: 25.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.353
IPC2_protein 5.41
IPC_protein 5.436
Toseland 5.842
ProMoST 5.779
Dawson 5.69
Bjellqvist 5.703
Wikipedia 5.703
Rodwell 5.677
Grimsley 5.982
Solomon 5.69
Lehninger 5.677
Nozaki 5.957
DTASelect 6.148
Thurlkill 6.148
EMBOSS 6.109
Sillero 6.046
Patrickios 0.973
IPC_peptide 5.703
IPC2_peptide 6.071
IPC2.peptide.svr19 6.0
Protein with the highest isoelectric point:
>tr|Q2V0R9|Q2V0R9_9VIRU Triple geneblock protein 1 OS=Nerine virus X OX=333348 PE=4 SV=1
MM1 pKa = 7.91 PLTAPPDD8 pKa = 3.67 YY9 pKa = 10.53 THH11 pKa = 7.51 ILPIAIVSIAVALSLYY27 pKa = 9.97 TITRR31 pKa = 11.84 NTLPHH36 pKa = 6.23 TGDD39 pKa = 4.17 NIHH42 pKa = 6.69 HH43 pKa = 6.66 FPHH46 pKa = 6.73 GGRR49 pKa = 11.84 YY50 pKa = 8.78 RR51 pKa = 11.84 DD52 pKa = 3.66 GTKK55 pKa = 10.39 SITYY59 pKa = 9.13 CPPQRR64 pKa = 11.84 NLSPFNSTGYY74 pKa = 10.48 SIIPTALAILLPAAIYY90 pKa = 10.46 LSSKK94 pKa = 9.81 CFNSRR99 pKa = 11.84 THH101 pKa = 6.05 PHH103 pKa = 6.03 SCSHH107 pKa = 6.31 CQPNSATMRR116 pKa = 11.84 GTSS119 pKa = 3.03
Molecular weight: 12.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.904
IPC2_protein 8.668
IPC_protein 8.843
Toseland 8.653
ProMoST 8.887
Dawson 9.107
Bjellqvist 9.297
Wikipedia 9.224
Rodwell 9.121
Grimsley 8.726
Solomon 9.326
Lehninger 9.297
Nozaki 9.355
DTASelect 9.092
Thurlkill 9.092
EMBOSS 9.253
Sillero 9.355
Patrickios 1.227
IPC_peptide 9.311
IPC2_peptide 8.785
IPC2.peptide.svr19 8.279
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2193
119
1504
438.6
49.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.664 ± 1.74
1.87 ± 0.499
4.514 ± 0.583
5.928 ± 1.336
3.694 ± 0.47
4.423 ± 0.225
4.15 ± 0.564
6.065 ± 0.76
5.016 ± 1.123
9.166 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.006 ± 0.387
4.606 ± 0.121
7.342 ± 0.729
4.514 ± 0.487
4.514 ± 0.271
7.068 ± 1.039
7.478 ± 0.568
4.651 ± 0.676
1.003 ± 0.258
3.329 ± 0.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here