Cucumber mosaic virus (strain FNY) (CMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Cucumovirus; Cucumber mosaic virus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17769|1A_CMVFN Replication protein 1a OS=Cucumber mosaic virus (strain FNY) OX=12307 GN=ORF1a PE=3 SV=2
MM1 pKa = 7.78AFPAPAFSLANLLNGSYY18 pKa = 10.84GVDD21 pKa = 3.06TPEE24 pKa = 3.93DD25 pKa = 3.67VEE27 pKa = 4.3RR28 pKa = 11.84LRR30 pKa = 11.84SEE32 pKa = 4.02QRR34 pKa = 11.84EE35 pKa = 4.09EE36 pKa = 3.47AAAACRR42 pKa = 11.84NYY44 pKa = 10.59RR45 pKa = 11.84PLPAVDD51 pKa = 3.51VSEE54 pKa = 4.73SVTEE58 pKa = 4.25DD59 pKa = 2.86AHH61 pKa = 7.17SLRR64 pKa = 11.84TPDD67 pKa = 3.59GAPAEE72 pKa = 4.26AVSDD76 pKa = 3.77EE77 pKa = 4.06FVTYY81 pKa = 10.12GAEE84 pKa = 4.21DD85 pKa = 3.96YY86 pKa = 11.25LEE88 pKa = 4.77KK89 pKa = 10.76SDD91 pKa = 6.3DD92 pKa = 3.73EE93 pKa = 4.54LLVAFEE99 pKa = 4.48TMVKK103 pKa = 9.87PMRR106 pKa = 11.84IGQLWCPAFNKK117 pKa = 10.21CSFISSIAMARR128 pKa = 11.84ALLLAPRR135 pKa = 11.84TSHH138 pKa = 6.16RR139 pKa = 11.84TMKK142 pKa = 10.54CFEE145 pKa = 4.89DD146 pKa = 4.26LVAAIYY152 pKa = 8.03TKK154 pKa = 10.88SDD156 pKa = 3.35FYY158 pKa = 11.69YY159 pKa = 10.59SEE161 pKa = 4.06EE162 pKa = 4.47CEE164 pKa = 4.84ADD166 pKa = 3.5DD167 pKa = 4.4AQIDD171 pKa = 3.63ISSRR175 pKa = 11.84DD176 pKa = 3.34VPGYY180 pKa = 10.54SFEE183 pKa = 4.16PWSRR187 pKa = 11.84TSGFEE192 pKa = 3.82PPPICEE198 pKa = 4.13ACDD201 pKa = 3.36MIMYY205 pKa = 8.23QCPCFDD211 pKa = 4.17FNALKK216 pKa = 10.42KK217 pKa = 10.37SCAEE221 pKa = 3.67RR222 pKa = 11.84TFADD226 pKa = 3.98DD227 pKa = 3.94YY228 pKa = 11.49VIEE231 pKa = 4.53GLDD234 pKa = 3.48GVVDD238 pKa = 3.83NATLLSNLGPFLVPVKK254 pKa = 10.21CQYY257 pKa = 9.26EE258 pKa = 4.27KK259 pKa = 11.27CPTPTIAIPPDD270 pKa = 3.47LNRR273 pKa = 11.84ATDD276 pKa = 3.45RR277 pKa = 11.84VDD279 pKa = 2.99INLVQSICDD288 pKa = 3.54STLPTHH294 pKa = 6.86SNYY297 pKa = 10.5DD298 pKa = 3.51DD299 pKa = 4.74SFHH302 pKa = 6.52QVFVEE307 pKa = 4.17SADD310 pKa = 3.56YY311 pKa = 11.12SIDD314 pKa = 3.74LDD316 pKa = 3.81HH317 pKa = 7.21VRR319 pKa = 11.84LRR321 pKa = 11.84QSDD324 pKa = 5.26LIAKK328 pKa = 9.64IPDD331 pKa = 3.93SGHH334 pKa = 6.42MIPVLNTGSGHH345 pKa = 5.95KK346 pKa = 10.01RR347 pKa = 11.84VGTTKK352 pKa = 10.48EE353 pKa = 3.68VLTAIKK359 pKa = 9.97KK360 pKa = 10.04RR361 pKa = 11.84NADD364 pKa = 3.49VPEE367 pKa = 5.43LGDD370 pKa = 3.75SVNLSRR376 pKa = 11.84LSKK379 pKa = 10.61AVAEE383 pKa = 4.47RR384 pKa = 11.84FFISYY389 pKa = 10.67INGNSLASSNFVNVVSNFHH408 pKa = 7.53DD409 pKa = 4.25YY410 pKa = 9.42MEE412 pKa = 4.41KK413 pKa = 9.79WKK415 pKa = 11.04SSGLSYY421 pKa = 11.44DD422 pKa = 4.55DD423 pKa = 5.94LPDD426 pKa = 3.54LHH428 pKa = 8.26AEE430 pKa = 3.82NLQFYY435 pKa = 10.96DD436 pKa = 4.19HH437 pKa = 6.9MIKK440 pKa = 10.37SDD442 pKa = 3.66VKK444 pKa = 10.5PVVSDD449 pKa = 3.14TLNIDD454 pKa = 3.49RR455 pKa = 11.84PVPATITYY463 pKa = 8.38HH464 pKa = 6.23KK465 pKa = 10.59KK466 pKa = 10.61SITSQFSPLFTALFEE481 pKa = 4.23RR482 pKa = 11.84FQRR485 pKa = 11.84CLRR488 pKa = 11.84EE489 pKa = 4.2RR490 pKa = 11.84IILPVGKK497 pKa = 10.03ISSLEE502 pKa = 3.74MAGFDD507 pKa = 4.72VKK509 pKa = 10.94NKK511 pKa = 9.77HH512 pKa = 6.23CLEE515 pKa = 4.69IDD517 pKa = 3.33LSKK520 pKa = 10.66FDD522 pKa = 3.64KK523 pKa = 11.1SQGEE527 pKa = 3.95FHH529 pKa = 7.12LLIQEE534 pKa = 4.92HH535 pKa = 6.57ILNGLGCPAPITKK548 pKa = 8.63WWCDD552 pKa = 3.85FHH554 pKa = 8.06RR555 pKa = 11.84FSYY558 pKa = 10.5IRR560 pKa = 11.84DD561 pKa = 3.2RR562 pKa = 11.84RR563 pKa = 11.84AGVGMPISFQRR574 pKa = 11.84RR575 pKa = 11.84TGDD578 pKa = 3.01ALTYY582 pKa = 10.06FGNTIVTMAEE592 pKa = 4.01FAWCYY597 pKa = 10.05DD598 pKa = 2.99TDD600 pKa = 3.72QFEE603 pKa = 4.62KK604 pKa = 11.13LLFSGDD610 pKa = 3.73DD611 pKa = 3.4SLGFSLLPPVGDD623 pKa = 3.38PSKK626 pKa = 10.39FTTLFNMEE634 pKa = 4.46AKK636 pKa = 10.42VMEE639 pKa = 4.39PAVPYY644 pKa = 10.13ICSKK648 pKa = 10.72FLLSDD653 pKa = 3.29EE654 pKa = 4.73FGNTFSVPDD663 pKa = 4.32PLRR666 pKa = 11.84EE667 pKa = 3.99VQRR670 pKa = 11.84LGTKK674 pKa = 10.07KK675 pKa = 10.14IPYY678 pKa = 9.12SDD680 pKa = 3.31NDD682 pKa = 3.45EE683 pKa = 4.15FLFAHH688 pKa = 6.5FMSFVDD694 pKa = 3.6RR695 pKa = 11.84LKK697 pKa = 11.07FLDD700 pKa = 5.08RR701 pKa = 11.84MSQSCIDD708 pKa = 3.38QLSIFFEE715 pKa = 4.35LKK717 pKa = 9.19YY718 pKa = 10.58KK719 pKa = 10.79KK720 pKa = 10.32SGEE723 pKa = 4.03EE724 pKa = 3.53AALMLGAFKK733 pKa = 10.76KK734 pKa = 9.49YY735 pKa = 7.71TANFQSYY742 pKa = 9.82KK743 pKa = 9.9EE744 pKa = 4.4LYY746 pKa = 9.56YY747 pKa = 10.53SDD749 pKa = 5.0RR750 pKa = 11.84RR751 pKa = 11.84QCEE754 pKa = 4.51LINSFCSTEE763 pKa = 3.83FRR765 pKa = 11.84VEE767 pKa = 3.76RR768 pKa = 11.84VNSNKK773 pKa = 8.44QRR775 pKa = 11.84KK776 pKa = 8.62NYY778 pKa = 10.18GIEE781 pKa = 3.73RR782 pKa = 11.84RR783 pKa = 11.84CNDD786 pKa = 2.98KK787 pKa = 10.85RR788 pKa = 11.84RR789 pKa = 11.84TPTGSYY795 pKa = 10.66GGGEE799 pKa = 3.92EE800 pKa = 5.26AEE802 pKa = 4.51TKK804 pKa = 10.38VSQTEE809 pKa = 4.25STGTRR814 pKa = 11.84SQKK817 pKa = 8.05SQRR820 pKa = 11.84EE821 pKa = 4.28SAFKK825 pKa = 10.74SQTIPLPTVLSSGWFGTDD843 pKa = 2.14RR844 pKa = 11.84VMPPCEE850 pKa = 3.87RR851 pKa = 11.84GGVTRR856 pKa = 11.84VV857 pKa = 2.88

Molecular weight: 96.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q00271|MVP_CMVFN Movement protein OS=Cucumber mosaic virus (strain FNY) OX=12307 GN=ORF3a PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 4.94KK3 pKa = 11.01SEE5 pKa = 4.19STSAGRR11 pKa = 11.84NRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84RR19 pKa = 11.84GSRR22 pKa = 11.84SAPSSADD29 pKa = 2.97ANFRR33 pKa = 11.84VLSQQLSRR41 pKa = 11.84LNKK44 pKa = 8.14TLAAGRR50 pKa = 11.84PTINHH55 pKa = 5.52PTFVGSEE62 pKa = 4.04RR63 pKa = 11.84CRR65 pKa = 11.84PGYY68 pKa = 9.09TFTSITLKK76 pKa = 10.38PPKK79 pKa = 9.04IDD81 pKa = 3.14RR82 pKa = 11.84GSYY85 pKa = 7.96YY86 pKa = 10.78GKK88 pKa = 10.51RR89 pKa = 11.84LLLPDD94 pKa = 4.13SVTEE98 pKa = 3.94YY99 pKa = 11.07DD100 pKa = 3.37KK101 pKa = 11.59KK102 pKa = 10.76LVSRR106 pKa = 11.84IQIRR110 pKa = 11.84VNPLPKK116 pKa = 9.66FDD118 pKa = 3.58STVWVTVRR126 pKa = 11.84KK127 pKa = 10.07VPASSDD133 pKa = 3.39LSVAAISAMFADD145 pKa = 5.23GASPVLVYY153 pKa = 9.71QYY155 pKa = 11.09AASGVQANNKK165 pKa = 9.21LLYY168 pKa = 10.24DD169 pKa = 4.23LSAMRR174 pKa = 11.84ADD176 pKa = 3.22IGDD179 pKa = 3.54MRR181 pKa = 11.84KK182 pKa = 9.47YY183 pKa = 10.88AVLVYY188 pKa = 10.8SKK190 pKa = 11.21DD191 pKa = 3.59DD192 pKa = 3.71ALEE195 pKa = 4.01TDD197 pKa = 3.9EE198 pKa = 6.0LVLHH202 pKa = 6.52VDD204 pKa = 3.74IEE206 pKa = 4.39HH207 pKa = 6.04QRR209 pKa = 11.84IPTSGVLPVV218 pKa = 3.49

Molecular weight: 24.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2457

110

993

491.4

55.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.367 ± 0.465

2.401 ± 0.323

6.349 ± 0.489

5.983 ± 0.618

4.843 ± 0.7

5.413 ± 0.209

2.239 ± 0.222

4.762 ± 0.468

5.454 ± 0.266

8.262 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.316

3.663 ± 0.298

4.477 ± 0.674

3.378 ± 0.257

6.431 ± 0.671

9.28 ± 0.391

6.064 ± 0.499

6.919 ± 0.492

1.018 ± 0.153

3.093 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski