Mycoplasma haemolamae (strain Purdue)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Candidatus Mycoplasma haemolamae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7C6T0|I7C6T0_MYCHA Uncharacterized protein OS=Mycoplasma haemolamae (strain Purdue) OX=1212765 GN=MHLP_03310 PE=4 SV=1
MM1 pKa = 7.27IVANKK6 pKa = 9.84VLTALSCCTAVGAGPYY22 pKa = 8.9GVAMVSSNAVSAPPAQGRR40 pKa = 11.84QSFGEE45 pKa = 4.3TCLLDD50 pKa = 4.39AKK52 pKa = 9.78TCAILAEE59 pKa = 4.29DD60 pKa = 4.46TEE62 pKa = 4.7PGEE65 pKa = 4.32DD66 pKa = 3.53TEE68 pKa = 4.66EE69 pKa = 5.64GDD71 pKa = 4.15VYY73 pKa = 11.4SEE75 pKa = 4.13TSDD78 pKa = 4.26EE79 pKa = 5.19DD80 pKa = 3.62FCGWGFEE87 pKa = 4.41FLKK90 pKa = 10.88EE91 pKa = 3.65MNTWKK96 pKa = 10.56KK97 pKa = 10.85CIGLDD102 pKa = 4.59KK103 pKa = 11.08EE104 pKa = 4.51EE105 pKa = 3.94QQ106 pKa = 3.25

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7CKD0|I7CKD0_MYCHA Uncharacterized protein OS=Mycoplasma haemolamae (strain Purdue) OX=1212765 GN=MHLP_03870 PE=4 SV=1
MM1 pKa = 7.2SVAYY5 pKa = 9.83LWLKK9 pKa = 10.25GLSAQGKK16 pKa = 8.91IYY18 pKa = 10.69LATSSLAIGSGITGTLAVDD37 pKa = 3.8STRR40 pKa = 11.84DD41 pKa = 3.84SIWQGVSYY49 pKa = 10.8VSSAASNLLGQAFKK63 pKa = 11.07VGNAPPRR70 pKa = 11.84QLRR73 pKa = 11.84EE74 pKa = 4.13FRR76 pKa = 11.84CQSQWNLQRR85 pKa = 11.84CLRR88 pKa = 11.84WSQDD92 pKa = 3.19LGCRR96 pKa = 11.84RR97 pKa = 11.84SS98 pKa = 3.17

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

923

0

923

215198

37

2653

233.2

26.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.43 ± 0.066

1.502 ± 0.045

4.844 ± 0.055

7.925 ± 0.103

4.685 ± 0.071

6.404 ± 0.097

1.483 ± 0.033

5.857 ± 0.086

8.994 ± 0.084

10.22 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.498 ± 0.029

4.302 ± 0.051

3.412 ± 0.052

3.883 ± 0.057

4.079 ± 0.062

9.284 ± 0.09

5.405 ± 0.067

5.834 ± 0.064

1.448 ± 0.041

3.51 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski