Amino acid dipepetide frequency for Vibrio phage ICP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.633AlaAla: 3.633 ± 0.43
0.813AlaCys: 0.813 ± 0.121
2.738AlaAsp: 2.738 ± 0.276
3.606AlaGlu: 3.606 ± 0.365
2.088AlaPhe: 2.088 ± 0.198
2.738AlaGly: 2.738 ± 0.261
1.003AlaHis: 1.003 ± 0.158
3.66AlaIle: 3.66 ± 0.309
3.796AlaLys: 3.796 ± 0.386
4.745AlaLeu: 4.745 ± 0.423
1.193AlaMet: 1.193 ± 0.191
2.25AlaAsn: 2.25 ± 0.256
1.301AlaPro: 1.301 ± 0.204
1.573AlaGln: 1.573 ± 0.252
2.006AlaArg: 2.006 ± 0.238
3.091AlaSer: 3.091 ± 0.345
3.118AlaThr: 3.118 ± 0.301
2.738AlaVal: 2.738 ± 0.303
0.84AlaTrp: 0.84 ± 0.134
1.952AlaTyr: 1.952 ± 0.247
0.0AlaXaa: 0.0 ± 0.0
Cys
0.84CysAla: 0.84 ± 0.148
0.434CysCys: 0.434 ± 0.099
0.895CysAsp: 0.895 ± 0.152
1.6CysGlu: 1.6 ± 0.202
0.705CysPhe: 0.705 ± 0.138
1.762CysGly: 1.762 ± 0.242
0.38CysHis: 0.38 ± 0.119
1.057CysIle: 1.057 ± 0.173
1.789CysLys: 1.789 ± 0.241
0.922CysLeu: 0.922 ± 0.206
0.569CysMet: 0.569 ± 0.125
0.922CysAsn: 0.922 ± 0.162
0.759CysPro: 0.759 ± 0.159
0.515CysGln: 0.515 ± 0.122
0.84CysArg: 0.84 ± 0.138
1.274CysSer: 1.274 ± 0.25
0.949CysThr: 0.949 ± 0.175
1.247CysVal: 1.247 ± 0.191
0.244CysTrp: 0.244 ± 0.078
0.705CysTyr: 0.705 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
2.386AspAla: 2.386 ± 0.253
1.383AspCys: 1.383 ± 0.168
3.47AspAsp: 3.47 ± 0.343
4.691AspGlu: 4.691 ± 0.362
3.281AspPhe: 3.281 ± 0.324
4.745AspGly: 4.745 ± 0.368
0.976AspHis: 0.976 ± 0.165
4.772AspIle: 4.772 ± 0.372
5.504AspLys: 5.504 ± 0.408
5.667AspLeu: 5.667 ± 0.399
1.681AspMet: 1.681 ± 0.225
3.606AspAsn: 3.606 ± 0.292
1.735AspPro: 1.735 ± 0.256
1.654AspGln: 1.654 ± 0.26
2.684AspArg: 2.684 ± 0.262
3.823AspSer: 3.823 ± 0.324
3.579AspThr: 3.579 ± 0.319
4.202AspVal: 4.202 ± 0.335
1.166AspTrp: 1.166 ± 0.169
3.85AspTyr: 3.85 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
4.175GluAla: 4.175 ± 0.364
1.356GluCys: 1.356 ± 0.224
6.127GluAsp: 6.127 ± 0.383
7.944GluGlu: 7.944 ± 0.567
3.443GluPhe: 3.443 ± 0.303
5.097GluGly: 5.097 ± 0.359
1.464GluHis: 1.464 ± 0.214
4.718GluIle: 4.718 ± 0.404
5.314GluLys: 5.314 ± 0.402
6.534GluLeu: 6.534 ± 0.418
2.25GluMet: 2.25 ± 0.247
3.66GluAsn: 3.66 ± 0.265
1.573GluPro: 1.573 ± 0.245
2.63GluGln: 2.63 ± 0.297
2.982GluArg: 2.982 ± 0.332
4.935GluSer: 4.935 ± 0.384
3.118GluThr: 3.118 ± 0.24
5.965GluVal: 5.965 ± 0.46
1.301GluTrp: 1.301 ± 0.221
3.986GluTyr: 3.986 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
1.898PheAla: 1.898 ± 0.299
0.786PheCys: 0.786 ± 0.145
3.796PheAsp: 3.796 ± 0.255
3.606PheGlu: 3.606 ± 0.296
1.383PhePhe: 1.383 ± 0.207
2.738PheGly: 2.738 ± 0.315
0.949PheHis: 0.949 ± 0.152
2.874PheIle: 2.874 ± 0.294
3.525PheLys: 3.525 ± 0.286
3.037PheLeu: 3.037 ± 0.271
0.868PheMet: 0.868 ± 0.14
2.115PheAsn: 2.115 ± 0.215
1.356PhePro: 1.356 ± 0.204
1.329PheGln: 1.329 ± 0.212
1.708PheArg: 1.708 ± 0.209
3.01PheSer: 3.01 ± 0.301
3.037PheThr: 3.037 ± 0.275
2.901PheVal: 2.901 ± 0.256
0.732PheTrp: 0.732 ± 0.144
1.925PheTyr: 1.925 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
2.793GlyAla: 2.793 ± 0.296
1.383GlyCys: 1.383 ± 0.218
4.365GlyAsp: 4.365 ± 0.336
4.88GlyGlu: 4.88 ± 0.328
3.064GlyPhe: 3.064 ± 0.282
3.796GlyGly: 3.796 ± 0.355
0.813GlyHis: 0.813 ± 0.15
3.443GlyIle: 3.443 ± 0.327
5.206GlyLys: 5.206 ± 0.439
4.528GlyLeu: 4.528 ± 0.362
1.301GlyMet: 1.301 ± 0.156
3.416GlyAsn: 3.416 ± 0.279
0.217GlyPro: 0.217 ± 0.09
1.735GlyGln: 1.735 ± 0.241
2.576GlyArg: 2.576 ± 0.3
3.877GlySer: 3.877 ± 0.319
3.525GlyThr: 3.525 ± 0.34
5.477GlyVal: 5.477 ± 0.392
1.573GlyTrp: 1.573 ± 0.201
3.823GlyTyr: 3.823 ± 0.312
0.0GlyXaa: 0.0 ± 0.0
His
0.651HisAla: 0.651 ± 0.122
0.515HisCys: 0.515 ± 0.114
0.922HisAsp: 0.922 ± 0.156
1.356HisGlu: 1.356 ± 0.162
0.949HisPhe: 0.949 ± 0.15
1.166HisGly: 1.166 ± 0.145
0.515HisHis: 0.515 ± 0.12
1.573HisIle: 1.573 ± 0.216
1.952HisLys: 1.952 ± 0.227
1.518HisLeu: 1.518 ± 0.21
0.569HisMet: 0.569 ± 0.104
1.03HisAsn: 1.03 ± 0.158
0.651HisPro: 0.651 ± 0.129
0.922HisGln: 0.922 ± 0.197
0.895HisArg: 0.895 ± 0.186
1.166HisSer: 1.166 ± 0.197
1.464HisThr: 1.464 ± 0.209
0.895HisVal: 0.895 ± 0.17
0.244HisTrp: 0.244 ± 0.075
0.868HisTyr: 0.868 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
3.335IleAla: 3.335 ± 0.315
0.868IleCys: 0.868 ± 0.135
4.935IleAsp: 4.935 ± 0.377
4.691IleGlu: 4.691 ± 0.379
2.169IlePhe: 2.169 ± 0.248
3.416IleGly: 3.416 ± 0.288
1.166IleHis: 1.166 ± 0.177
4.311IleIle: 4.311 ± 0.39
6.182IleLys: 6.182 ± 0.37
5.097IleLeu: 5.097 ± 0.343
1.166IleMet: 1.166 ± 0.164
3.66IleAsn: 3.66 ± 0.288
2.603IlePro: 2.603 ± 0.242
1.844IleGln: 1.844 ± 0.223
3.226IleArg: 3.226 ± 0.264
3.769IleSer: 3.769 ± 0.295
3.769IleThr: 3.769 ± 0.357
4.311IleVal: 4.311 ± 0.363
0.515IleTrp: 0.515 ± 0.123
2.82IleTyr: 2.82 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
4.148LysAla: 4.148 ± 0.36
1.383LysCys: 1.383 ± 0.185
5.639LysAsp: 5.639 ± 0.341
6.317LysGlu: 6.317 ± 0.381
3.281LysPhe: 3.281 ± 0.33
5.341LysGly: 5.341 ± 0.403
2.115LysHis: 2.115 ± 0.245
5.124LysIle: 5.124 ± 0.423
4.555LysLys: 4.555 ± 0.359
7.592LysLeu: 7.592 ± 0.549
2.223LysMet: 2.223 ± 0.264
3.145LysAsn: 3.145 ± 0.309
2.115LysPro: 2.115 ± 0.237
3.118LysGln: 3.118 ± 0.322
4.175LysArg: 4.175 ± 0.336
5.694LysSer: 5.694 ± 0.402
3.742LysThr: 3.742 ± 0.301
5.612LysVal: 5.612 ± 0.414
1.085LysTrp: 1.085 ± 0.19
4.311LysTyr: 4.311 ± 0.381
0.0LysXaa: 0.0 ± 0.0
Leu
4.446LeuAla: 4.446 ± 0.379
1.573LeuCys: 1.573 ± 0.197
5.748LeuAsp: 5.748 ± 0.479
7.076LeuGlu: 7.076 ± 0.466
3.091LeuPhe: 3.091 ± 0.276
5.341LeuGly: 5.341 ± 0.423
1.573LeuHis: 1.573 ± 0.194
4.23LeuIle: 4.23 ± 0.344
6.561LeuLys: 6.561 ± 0.418
7.076LeuLeu: 7.076 ± 0.525
2.277LeuMet: 2.277 ± 0.269
4.419LeuAsn: 4.419 ± 0.391
2.901LeuPro: 2.901 ± 0.307
2.82LeuGln: 2.82 ± 0.277
3.742LeuArg: 3.742 ± 0.329
6.832LeuSer: 6.832 ± 0.487
4.582LeuThr: 4.582 ± 0.386
5.233LeuVal: 5.233 ± 0.366
1.329LeuTrp: 1.329 ± 0.209
3.66LeuTyr: 3.66 ± 0.358
0.0LeuXaa: 0.0 ± 0.0
Met
1.708MetAla: 1.708 ± 0.225
0.461MetCys: 0.461 ± 0.111
1.274MetAsp: 1.274 ± 0.194
1.6MetGlu: 1.6 ± 0.223
1.03MetPhe: 1.03 ± 0.162
1.03MetGly: 1.03 ± 0.165
0.461MetHis: 0.461 ± 0.108
1.735MetIle: 1.735 ± 0.228
2.521MetLys: 2.521 ± 0.27
1.762MetLeu: 1.762 ± 0.221
0.786MetMet: 0.786 ± 0.159
1.735MetAsn: 1.735 ± 0.238
0.759MetPro: 0.759 ± 0.124
1.22MetGln: 1.22 ± 0.178
1.193MetArg: 1.193 ± 0.222
2.25MetSer: 2.25 ± 0.252
1.573MetThr: 1.573 ± 0.229
1.193MetVal: 1.193 ± 0.189
0.136MetTrp: 0.136 ± 0.045
0.976MetTyr: 0.976 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
2.277AsnAla: 2.277 ± 0.262
0.569AsnCys: 0.569 ± 0.131
2.928AsnAsp: 2.928 ± 0.268
2.711AsnGlu: 2.711 ± 0.265
2.549AsnPhe: 2.549 ± 0.251
3.823AsnGly: 3.823 ± 0.315
1.41AsnHis: 1.41 ± 0.22
4.067AsnIle: 4.067 ± 0.358
4.446AsnLys: 4.446 ± 0.384
4.853AsnLeu: 4.853 ± 0.381
1.166AsnMet: 1.166 ± 0.181
3.091AsnAsn: 3.091 ± 0.304
2.223AsnPro: 2.223 ± 0.233
2.088AsnGln: 2.088 ± 0.234
1.925AsnArg: 1.925 ± 0.259
3.199AsnSer: 3.199 ± 0.31
3.064AsnThr: 3.064 ± 0.289
2.467AsnVal: 2.467 ± 0.235
0.732AsnTrp: 0.732 ± 0.145
2.413AsnTyr: 2.413 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
1.6ProAla: 1.6 ± 0.23
0.596ProCys: 0.596 ± 0.131
2.142ProAsp: 2.142 ± 0.232
2.901ProGlu: 2.901 ± 0.249
1.573ProPhe: 1.573 ± 0.207
0.081ProGly: 0.081 ± 0.057
1.085ProHis: 1.085 ± 0.174
1.6ProIle: 1.6 ± 0.187
2.088ProLys: 2.088 ± 0.248
2.006ProLeu: 2.006 ± 0.28
0.732ProMet: 0.732 ± 0.126
1.41ProAsn: 1.41 ± 0.199
0.84ProPro: 0.84 ± 0.2
1.193ProGln: 1.193 ± 0.195
0.949ProArg: 0.949 ± 0.151
1.952ProSer: 1.952 ± 0.236
1.789ProThr: 1.789 ± 0.206
2.277ProVal: 2.277 ± 0.296
0.488ProTrp: 0.488 ± 0.116
1.383ProTyr: 1.383 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
2.006GlnAla: 2.006 ± 0.281
0.515GlnCys: 0.515 ± 0.118
1.952GlnAsp: 1.952 ± 0.265
3.579GlnGlu: 3.579 ± 0.381
1.085GlnPhe: 1.085 ± 0.18
2.088GlnGly: 2.088 ± 0.275
0.786GlnHis: 0.786 ± 0.136
2.033GlnIle: 2.033 ± 0.217
2.684GlnLys: 2.684 ± 0.283
2.521GlnLeu: 2.521 ± 0.284
0.786GlnMet: 0.786 ± 0.124
1.681GlnAsn: 1.681 ± 0.217
0.84GlnPro: 0.84 ± 0.141
1.817GlnGln: 1.817 ± 0.241
1.735GlnArg: 1.735 ± 0.217
2.169GlnSer: 2.169 ± 0.211
1.654GlnThr: 1.654 ± 0.226
2.25GlnVal: 2.25 ± 0.229
0.488GlnTrp: 0.488 ± 0.116
1.573GlnTyr: 1.573 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
1.735ArgAla: 1.735 ± 0.229
0.895ArgCys: 0.895 ± 0.145
2.521ArgAsp: 2.521 ± 0.273
3.389ArgGlu: 3.389 ± 0.333
2.115ArgPhe: 2.115 ± 0.225
2.657ArgGly: 2.657 ± 0.281
0.705ArgHis: 0.705 ± 0.141
2.576ArgIle: 2.576 ± 0.236
3.606ArgLys: 3.606 ± 0.388
4.202ArgLeu: 4.202 ± 0.34
1.274ArgMet: 1.274 ± 0.172
2.223ArgAsn: 2.223 ± 0.223
1.22ArgPro: 1.22 ± 0.15
1.708ArgGln: 1.708 ± 0.202
1.6ArgArg: 1.6 ± 0.236
2.359ArgSer: 2.359 ± 0.254
2.061ArgThr: 2.061 ± 0.238
2.901ArgVal: 2.901 ± 0.315
0.732ArgTrp: 0.732 ± 0.122
1.952ArgTyr: 1.952 ± 0.207
0.0ArgXaa: 0.0 ± 0.0
Ser
2.955SerAla: 2.955 ± 0.306
1.057SerCys: 1.057 ± 0.193
4.175SerAsp: 4.175 ± 0.343
4.935SerGlu: 4.935 ± 0.328
3.091SerPhe: 3.091 ± 0.314
5.07SerGly: 5.07 ± 0.383
1.057SerHis: 1.057 ± 0.157
3.769SerIle: 3.769 ± 0.304
6.073SerLys: 6.073 ± 0.402
5.667SerLeu: 5.667 ± 0.426
1.518SerMet: 1.518 ± 0.199
3.308SerAsn: 3.308 ± 0.278
1.627SerPro: 1.627 ± 0.193
1.952SerGln: 1.952 ± 0.208
2.738SerArg: 2.738 ± 0.287
4.419SerSer: 4.419 ± 0.344
3.064SerThr: 3.064 ± 0.279
4.365SerVal: 4.365 ± 0.423
1.193SerTrp: 1.193 ± 0.167
3.389SerTyr: 3.389 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
3.01ThrAla: 3.01 ± 0.344
1.03ThrCys: 1.03 ± 0.163
2.332ThrAsp: 2.332 ± 0.29
3.687ThrGlu: 3.687 ± 0.367
2.874ThrPhe: 2.874 ± 0.307
3.633ThrGly: 3.633 ± 0.353
1.085ThrHis: 1.085 ± 0.162
4.419ThrIle: 4.419 ± 0.37
4.121ThrLys: 4.121 ± 0.35
5.585ThrLeu: 5.585 ± 0.364
1.437ThrMet: 1.437 ± 0.205
3.037ThrAsn: 3.037 ± 0.313
2.657ThrPro: 2.657 ± 0.259
1.518ThrGln: 1.518 ± 0.162
2.142ThrArg: 2.142 ± 0.25
3.281ThrSer: 3.281 ± 0.316
3.308ThrThr: 3.308 ± 0.35
3.714ThrVal: 3.714 ± 0.314
0.759ThrTrp: 0.759 ± 0.134
2.494ThrTyr: 2.494 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
3.091ValAla: 3.091 ± 0.306
1.22ValCys: 1.22 ± 0.231
4.691ValAsp: 4.691 ± 0.327
5.667ValGlu: 5.667 ± 0.4
2.847ValPhe: 2.847 ± 0.306
3.986ValGly: 3.986 ± 0.331
0.813ValHis: 0.813 ± 0.154
4.067ValIle: 4.067 ± 0.395
5.612ValLys: 5.612 ± 0.432
5.341ValLeu: 5.341 ± 0.377
1.871ValMet: 1.871 ± 0.198
3.769ValAsn: 3.769 ± 0.36
1.573ValPro: 1.573 ± 0.207
2.305ValGln: 2.305 ± 0.255
2.413ValArg: 2.413 ± 0.244
4.148ValSer: 4.148 ± 0.287
4.609ValThr: 4.609 ± 0.418
5.206ValVal: 5.206 ± 0.418
0.868ValTrp: 0.868 ± 0.16
3.199ValTyr: 3.199 ± 0.242
0.0ValXaa: 0.0 ± 0.0
Trp
0.325TrpAla: 0.325 ± 0.097
0.434TrpCys: 0.434 ± 0.127
1.166TrpAsp: 1.166 ± 0.177
1.301TrpGlu: 1.301 ± 0.195
0.569TrpPhe: 0.569 ± 0.138
0.488TrpGly: 0.488 ± 0.105
0.217TrpHis: 0.217 ± 0.071
0.84TrpIle: 0.84 ± 0.13
1.627TrpLys: 1.627 ± 0.199
1.545TrpLeu: 1.545 ± 0.217
0.542TrpMet: 0.542 ± 0.117
0.84TrpAsn: 0.84 ± 0.179
0.217TrpPro: 0.217 ± 0.079
0.569TrpGln: 0.569 ± 0.129
0.515TrpArg: 0.515 ± 0.109
0.922TrpSer: 0.922 ± 0.172
0.678TrpThr: 0.678 ± 0.142
1.464TrpVal: 1.464 ± 0.203
0.217TrpTrp: 0.217 ± 0.079
0.678TrpTyr: 0.678 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.871TyrAla: 1.871 ± 0.254
1.139TyrCys: 1.139 ± 0.188
3.037TyrAsp: 3.037 ± 0.31
3.037TyrGlu: 3.037 ± 0.269
2.386TyrPhe: 2.386 ± 0.301
2.793TyrGly: 2.793 ± 0.266
1.247TyrHis: 1.247 ± 0.169
2.982TyrIle: 2.982 ± 0.248
3.579TyrLys: 3.579 ± 0.343
4.311TyrLeu: 4.311 ± 0.423
1.166TyrMet: 1.166 ± 0.166
2.684TyrAsn: 2.684 ± 0.314
1.518TyrPro: 1.518 ± 0.159
1.735TyrGln: 1.735 ± 0.228
2.386TyrArg: 2.386 ± 0.235
3.254TyrSer: 3.254 ± 0.331
3.443TyrThr: 3.443 ± 0.32
2.874TyrVal: 2.874 ± 0.339
0.515TyrTrp: 0.515 ± 0.109
2.305TyrTyr: 2.305 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 230 proteins (36884 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski