Arcanobacterium sp. S3PF19
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1003 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A095X7C1|A0A095X7C1_9ACTO Uncharacterized protein OS=Arcanobacterium sp. S3PF19 OX=1219585 GN=HMPREF1631_04110 PE=4 SV=1
MM1 pKa = 7.41 GFDD4 pKa = 3.34 ACAPLNLRR12 pKa = 11.84 RR13 pKa = 11.84 GCFVGGDD20 pKa = 3.8 GFALANFEE28 pKa = 4.32 LVEE31 pKa = 4.02 ALVVVVLVLGVMSVTGDD48 pKa = 3.61 GLVDD52 pKa = 3.48 EE53 pKa = 5.31 FGGYY57 pKa = 10.39 LDD59 pKa = 4.89 FACLLL64 pKa = 4.01
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 3.77
IPC_protein 3.605
Toseland 3.427
ProMoST 3.834
Dawson 3.63
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.35
Solomon 3.567
Lehninger 3.528
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.745
Patrickios 0.477
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A095ZAH6|A0A095ZAH6_9ACTO Elongation factor Ts OS=Arcanobacterium sp. S3PF19 OX=1219585 GN=tsf PE=3 SV=1
MM1 pKa = 6.54 GTVRR5 pKa = 11.84 GSAALCGHH13 pKa = 6.17 YY14 pKa = 10.72 VPLSANRR21 pKa = 11.84 AKK23 pKa = 10.57 KK24 pKa = 10.46 SVGSCVPAGKK34 pKa = 7.98 PTSEE38 pKa = 4.05 PARR41 pKa = 11.84 VKK43 pKa = 10.58 LRR45 pKa = 11.84 AVHH48 pKa = 6.47 SDD50 pKa = 2.86 EE51 pKa = 6.07 AYY53 pKa = 10.38 FPQVPAAGAGGRR65 pKa = 11.84 EE66 pKa = 3.87 DD67 pKa = 3.7 AAPNRR72 pKa = 11.84 IVRR75 pKa = 11.84 PDD77 pKa = 3.04 RR78 pKa = 11.84 RR79 pKa = 11.84 KK80 pKa = 9.06 SRR82 pKa = 11.84 RR83 pKa = 11.84 GIVLAVADD91 pKa = 4.81 GFAGEE96 pKa = 4.15 QFGVSAVRR104 pKa = 11.84 MPDD107 pKa = 3.53 LNRR110 pKa = 11.84 MPRR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 PVSPVRR121 pKa = 11.84 NSGGVSDD128 pKa = 5.18 DD129 pKa = 3.55 VSFLRR134 pKa = 11.84 SVVALCMSPAVKK146 pKa = 9.92 IAFTLLFFLLLFGLAVSCIDD166 pKa = 3.98 LLFGSVIYY174 pKa = 8.02 PTAGSAGPAASPLLLL189 pKa = 4.82
Molecular weight: 19.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.545
IPC_protein 10.365
Toseland 10.613
ProMoST 10.335
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.862
Grimsley 10.76
Solomon 10.847
Lehninger 10.818
Nozaki 10.628
DTASelect 10.438
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.847
IPC2_peptide 9.706
IPC2.peptide.svr19 8.56
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1003
0
1003
334713
51
2285
333.7
36.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.227 ± 0.096
1.088 ± 0.024
5.232 ± 0.058
6.225 ± 0.085
3.624 ± 0.056
8.332 ± 0.078
1.911 ± 0.033
5.384 ± 0.065
4.706 ± 0.062
9.609 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.171 ± 0.034
3.122 ± 0.048
4.766 ± 0.061
3.042 ± 0.042
6.846 ± 0.084
5.809 ± 0.059
5.536 ± 0.05
7.61 ± 0.071
1.175 ± 0.034
2.583 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here