Circoviridae 7 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNC7|S5TNC7_9CIRC Replication-associated protein OS=Circoviridae 7 LDMD-2013 OX=1379711 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84GNPGEE7 pKa = 4.28ASGEE11 pKa = 3.93ALDD14 pKa = 5.13DD15 pKa = 3.79VRR17 pKa = 11.84TLAVLGNWDD26 pKa = 3.55TTKK29 pKa = 11.12ANPAVTNYY37 pKa = 9.58PLSASNTISRR47 pKa = 11.84RR48 pKa = 11.84YY49 pKa = 9.87NGARR53 pKa = 11.84QQNWLNVEE61 pKa = 4.18PLSEE65 pKa = 5.08PGTSLYY71 pKa = 10.46GAPLPEE77 pKa = 4.27QYY79 pKa = 10.59DD80 pKa = 3.8VQVGKK85 pKa = 10.82GYY87 pKa = 10.32IEE89 pKa = 3.7FTVKK93 pKa = 10.87NEE95 pKa = 3.89GTRR98 pKa = 11.84QAVVEE103 pKa = 4.14LVLFKK108 pKa = 10.76PNKK111 pKa = 9.0NAFNKK116 pKa = 10.2PYY118 pKa = 10.28LIDD121 pKa = 3.87EE122 pKa = 4.74APSAEE127 pKa = 4.03IAPNIAEE134 pKa = 4.34NVWYY138 pKa = 9.17WLNRR142 pKa = 11.84QNGEE146 pKa = 4.07QYY148 pKa = 9.96VQNIMSTRR156 pKa = 11.84ARR158 pKa = 11.84ALAKK162 pKa = 10.16RR163 pKa = 11.84VGPTDD168 pKa = 3.36TNVPIDD174 pKa = 4.32KK175 pKa = 10.04PDD177 pKa = 3.73SNDD180 pKa = 3.14ILISPYY186 pKa = 11.11VNFLPEE192 pKa = 3.98SSFKK196 pKa = 11.08NVVGNSQAFKK206 pKa = 10.5RR207 pKa = 11.84PWMLDD212 pKa = 2.87GAGNGNVIVQSTVAEE227 pKa = 4.1EE228 pKa = 4.2MAASTSNIAEE238 pKa = 4.25VEE240 pKa = 4.05GGEE243 pKa = 4.58LNDD246 pKa = 4.15GEE248 pKa = 5.04AXGRR252 pKa = 11.84WKK254 pKa = 10.88NPYY257 pKa = 8.93TSIGRR262 pKa = 11.84AFAAVPAQGKK272 pKa = 7.47RR273 pKa = 11.84TIRR276 pKa = 11.84IPLPKK281 pKa = 9.92LRR283 pKa = 11.84YY284 pKa = 9.27NPSTSSDD291 pKa = 3.51YY292 pKa = 11.7SKK294 pKa = 11.34LDD296 pKa = 3.21DD297 pKa = 5.01DD298 pKa = 4.67FAIRR302 pKa = 11.84PQSGTSATLYY312 pKa = 7.03PTIMNPNSIMVAMSLNGARR331 pKa = 11.84SDD333 pKa = 3.79FFEE336 pKa = 4.75TKK338 pKa = 10.4DD339 pKa = 3.43SEE341 pKa = 4.51AKK343 pKa = 10.39FRR345 pKa = 11.84GQDD348 pKa = 3.12FTAAKK353 pKa = 9.82VYY355 pKa = 10.78VDD357 pKa = 3.74AVYY360 pKa = 10.86KK361 pKa = 8.92EE362 pKa = 4.45TVYY365 pKa = 10.62PCAMDD370 pKa = 3.55TQEE373 pKa = 4.64TKK375 pKa = 9.78TAFNFGTPYY384 pKa = 7.97FTNMGEE390 pKa = 4.29VPVGSSYY397 pKa = 10.56WPGVVQDD404 pKa = 4.24AANVQNTVSFGTSIGAGVVQTPGTT428 pKa = 3.63

Molecular weight: 46.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TMV3|S5TMV3_9CIRC Uncharacterized protein OS=Circoviridae 7 LDMD-2013 OX=1379711 PE=4 SV=1
MM1 pKa = 7.66GKK3 pKa = 9.73NDD5 pKa = 3.91TYY7 pKa = 10.69ICSKK11 pKa = 10.14KK12 pKa = 10.27RR13 pKa = 11.84CTFYY17 pKa = 10.61STILLTYY24 pKa = 8.58WSRR27 pKa = 11.84ARR29 pKa = 11.84MAWTRR34 pKa = 11.84GRR36 pKa = 11.84TRR38 pKa = 11.84SGAYY42 pKa = 9.04YY43 pKa = 9.98QAAEE47 pKa = 4.24QIADD51 pKa = 3.55EE52 pKa = 4.21YY53 pKa = 10.63LRR55 pKa = 11.84RR56 pKa = 11.84VGIQDD61 pKa = 2.73RR62 pKa = 11.84RR63 pKa = 11.84MRR65 pKa = 11.84QVAIAGITAGLQRR78 pKa = 11.84GVTNFRR84 pKa = 11.84GLIPYY89 pKa = 9.39IRR91 pKa = 11.84EE92 pKa = 4.19SLPSLPVVDD101 pKa = 4.69PLFMARR107 pKa = 11.84SFRR110 pKa = 11.84DD111 pKa = 3.17AMVSITGEE119 pKa = 3.94VTRR122 pKa = 11.84PSLSAPAPSTSSSVGAQGSNMTSPQTQTRR151 pKa = 11.84MLLRR155 pKa = 11.84RR156 pKa = 11.84PAPGSSTKK164 pKa = 7.98TVKK167 pKa = 9.89RR168 pKa = 11.84HH169 pKa = 4.83KK170 pKa = 10.56KK171 pKa = 8.29KK172 pKa = 11.23GNMSGLDD179 pKa = 3.6PNTVQTYY186 pKa = 9.21PRR188 pKa = 11.84LTVSMPTDD196 pKa = 3.21KK197 pKa = 10.8TLLRR201 pKa = 11.84SSFAQFKK208 pKa = 10.25KK209 pKa = 9.44WFDD212 pKa = 3.27GHH214 pKa = 6.7RR215 pKa = 11.84VLTNSFSFIMEE226 pKa = 3.92STQGKK231 pKa = 9.63RR232 pKa = 11.84GNLLIPLRR240 pKa = 11.84CDD242 pKa = 2.92PCIYY246 pKa = 10.46GGRR249 pKa = 11.84THH251 pKa = 6.52EE252 pKa = 5.27QDD254 pKa = 3.17TADD257 pKa = 3.08NYY259 pKa = 10.85YY260 pKa = 10.75RR261 pKa = 11.84FVTGASWGQDD271 pKa = 3.1VNLPDD276 pKa = 4.17PVDD279 pKa = 3.53GKK281 pKa = 10.74KK282 pKa = 10.19VQDD285 pKa = 3.46IGPAGHH291 pKa = 5.79YY292 pKa = 7.83QHH294 pKa = 6.8VVGDD298 pKa = 3.95LMRR301 pKa = 11.84TGYY304 pKa = 10.53SDD306 pKa = 3.45STVCEE311 pKa = 4.36PVTKK315 pKa = 10.6DD316 pKa = 3.65LDD318 pKa = 3.78LSIPGNSITQQTATAKK334 pKa = 10.5HH335 pKa = 5.99MIVPGLTASSFRR347 pKa = 11.84AGIMAVEE354 pKa = 4.24

Molecular weight: 39.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1568

153

428

313.6

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.334 ± 0.772

1.339 ± 0.52

4.911 ± 0.542

5.102 ± 0.751

3.827 ± 0.212

6.888 ± 0.577

1.467 ± 0.583

4.273 ± 0.364

4.592 ± 0.665

7.079 ± 1.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.506

5.293 ± 1.137

7.079 ± 0.329

3.763 ± 0.347

8.099 ± 1.803

7.079 ± 0.63

7.589 ± 0.647

6.378 ± 0.634

1.531 ± 0.129

3.444 ± 0.53
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski