Hubei picorna-like virus 15
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KM27|A0A1L3KM27_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 15 OX=1923094 PE=4 SV=1
MM1 pKa = 7.96 DD2 pKa = 4.98 CVNDD6 pKa = 3.89 GRR8 pKa = 11.84 SHH10 pKa = 6.71 NIISFLEE17 pKa = 4.06 RR18 pKa = 11.84 PIMMVTAAWPTTTEE32 pKa = 3.96 RR33 pKa = 11.84 GTVLQAFEE41 pKa = 5.4 LPWDD45 pKa = 3.69 MLFKK49 pKa = 11.03 DD50 pKa = 3.67 MYY52 pKa = 7.98 KK53 pKa = 9.64 TKK55 pKa = 10.44 VDD57 pKa = 3.03 RR58 pKa = 11.84 FYY60 pKa = 11.67 GFRR63 pKa = 11.84 ADD65 pKa = 3.78 CEE67 pKa = 3.74 IRR69 pKa = 11.84 VQVNSQPFQAGRR81 pKa = 11.84 LLLSWIPGYY90 pKa = 10.46 RR91 pKa = 11.84 YY92 pKa = 10.17 LGNKK96 pKa = 5.97 QQYY99 pKa = 8.68 YY100 pKa = 10.8 SSTTTSLATNVKK112 pKa = 9.13 YY113 pKa = 10.66 LPPITGSPRR122 pKa = 11.84 IDD124 pKa = 4.38 LDD126 pKa = 4.45 LSTCTEE132 pKa = 3.66 ATMCVPYY139 pKa = 10.37 ISPYY143 pKa = 10.58 SFSEE147 pKa = 3.96 LTNGIGSMGRR157 pKa = 11.84 FQLVVYY163 pKa = 10.36 SPLSDD168 pKa = 3.48 TQTGTVDD175 pKa = 3.06 YY176 pKa = 9.87 TIFMNFKK183 pKa = 10.06 NIQLRR188 pKa = 11.84 YY189 pKa = 7.07 PTGLPLTATAQIGSEE204 pKa = 4.0 AVEE207 pKa = 4.13 EE208 pKa = 4.19 AGGAGIITSTASAISTALGAVADD231 pKa = 4.17 IPGVSQFAQPALWVSKK247 pKa = 10.72 NIADD251 pKa = 4.01 VARR254 pKa = 11.84 QFGWSKK260 pKa = 8.43 PTSIEE265 pKa = 3.94 APHH268 pKa = 5.16 VTKK271 pKa = 10.94 LSSTRR276 pKa = 11.84 FMANSDD282 pKa = 3.79 GVDD285 pKa = 3.44 TSHH288 pKa = 6.96 VLSLLSDD295 pKa = 3.55 NSLEE299 pKa = 4.09 TDD301 pKa = 2.85 ASLFRR306 pKa = 11.84 TNVDD310 pKa = 2.93 EE311 pKa = 4.26 MALSHH316 pKa = 6.12 VARR319 pKa = 11.84 TQTFYY324 pKa = 11.2 TRR326 pKa = 11.84 FAWSTTATAGSVLFSAPITPNFYY349 pKa = 10.48 RR350 pKa = 11.84 HH351 pKa = 6.37 TISTAQYY358 pKa = 10.64 APTTLAYY365 pKa = 9.22 TSAAFRR371 pKa = 11.84 QWRR374 pKa = 11.84 GGINFSFKK382 pKa = 9.85 FVKK385 pKa = 9.89 TKK387 pKa = 9.72 FHH389 pKa = 6.54 SGRR392 pKa = 11.84 VRR394 pKa = 11.84 IIYY397 pKa = 10.06 VPGDD401 pKa = 3.47 YY402 pKa = 9.82 STGVSLPANFDD413 pKa = 2.74 IDD415 pKa = 4.44 ANYY418 pKa = 8.85 STVVDD423 pKa = 4.67 LRR425 pKa = 11.84 SDD427 pKa = 3.35 TDD429 pKa = 3.57 VEE431 pKa = 4.62 FNVPYY436 pKa = 10.62 VAIQQWLLVDD446 pKa = 3.5 NAYY449 pKa = 10.08 PGTARR454 pKa = 11.84 TNFFSTGTIYY464 pKa = 10.32 MVVLNEE470 pKa = 3.96 LRR472 pKa = 11.84 AVSAVASSIDD482 pKa = 3.63 VITEE486 pKa = 3.53 VGAASDD492 pKa = 4.21 FEE494 pKa = 4.65 LSIPRR499 pKa = 11.84 LPSIYY504 pKa = 9.89 PSDD507 pKa = 3.81 VLFPPPAPATGSVLNRR523 pKa = 11.84 LVRR526 pKa = 11.84 ATAQVGEE533 pKa = 4.72 SEE535 pKa = 5.27 AVMAPPEE542 pKa = 4.32 VIQASGAVTAPLTSKK557 pKa = 10.17 EE558 pKa = 4.22 FSGTTYY564 pKa = 10.48 VGGAITVGEE573 pKa = 4.37 KK574 pKa = 10.42 VSSIRR579 pKa = 11.84 QIIKK583 pKa = 10.02 RR584 pKa = 11.84 FHH586 pKa = 6.86 KK587 pKa = 10.15 IYY589 pKa = 10.79 SDD591 pKa = 3.4 AAAGNLITGSYY602 pKa = 8.64 QVQPSKK608 pKa = 11.39 VNSPLNSGSTLPRR621 pKa = 11.84 SIDD624 pKa = 3.19 MYY626 pKa = 11.16 DD627 pKa = 3.45 YY628 pKa = 11.5 YY629 pKa = 11.6 SYY631 pKa = 11.3 LYY633 pKa = 11.01 AFFRR637 pKa = 11.84 GSFRR641 pKa = 11.84 FKK643 pKa = 10.46 VLPYY647 pKa = 10.54 DD648 pKa = 3.6 FQIYY652 pKa = 9.0 AARR655 pKa = 11.84 VRR657 pKa = 11.84 LLPEE661 pKa = 3.56 QLITSGASPVDD672 pKa = 3.75 FVNALLPEE680 pKa = 4.47 YY681 pKa = 8.97 FTAADD686 pKa = 3.34 VYY688 pKa = 9.82 MPRR691 pKa = 11.84 NIEE694 pKa = 4.02 GVFEE698 pKa = 4.22 FQVPHH703 pKa = 5.99 YY704 pKa = 9.83 SRR706 pKa = 11.84 YY707 pKa = 9.53 PILPIIAGGNISSDD721 pKa = 2.9 LFQRR725 pKa = 11.84 NFTEE729 pKa = 4.4 IDD731 pKa = 3.42 LSTSSTNALLDD742 pKa = 3.39 RR743 pKa = 11.84 TRR745 pKa = 11.84 ITVYY749 pKa = 10.34 RR750 pKa = 11.84 AVGDD754 pKa = 3.81 DD755 pKa = 3.64 FSFNQLIGPPFVSQYY770 pKa = 9.89 TATT773 pKa = 3.65
Molecular weight: 84.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.51
IPC2_protein 5.626
IPC_protein 5.639
Toseland 5.944
ProMoST 5.919
Dawson 5.817
Bjellqvist 5.868
Wikipedia 5.804
Rodwell 5.779
Grimsley 6.071
Solomon 5.817
Lehninger 5.804
Nozaki 6.046
DTASelect 6.249
Thurlkill 6.173
EMBOSS 6.135
Sillero 6.135
Patrickios 3.719
IPC_peptide 5.83
IPC2_peptide 6.135
IPC2.peptide.svr19 6.137
Protein with the highest isoelectric point:
>tr|A0A1L3KM27|A0A1L3KM27_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 15 OX=1923094 PE=4 SV=1
MM1 pKa = 7.48 SEE3 pKa = 4.56 AFSTMLPRR11 pKa = 11.84 LALQLSTPEE20 pKa = 3.72 INQLKK25 pKa = 10.07 IRR27 pKa = 11.84 DD28 pKa = 3.91 AQTQEE33 pKa = 3.78 KK34 pKa = 8.95 PRR36 pKa = 11.84 LVAQLTRR43 pKa = 11.84 SALNNMNNVYY53 pKa = 10.61 IMTDD57 pKa = 2.98 VLDD60 pKa = 5.64 AIPEE64 pKa = 4.0 NTLRR68 pKa = 11.84 KK69 pKa = 9.2 QVLAYY74 pKa = 8.39 ITEE77 pKa = 4.53 FNEE80 pKa = 3.87 SHH82 pKa = 6.45 NGISRR87 pKa = 11.84 PSRR90 pKa = 11.84 IAQLDD95 pKa = 3.67 GNIAYY100 pKa = 7.66 LTLYY104 pKa = 10.59 LDD106 pKa = 4.35 YY107 pKa = 11.36 LMQHH111 pKa = 7.0 LKK113 pKa = 8.4 THH115 pKa = 5.68 SQVKK119 pKa = 9.99 DD120 pKa = 3.41 CFFIMLKK127 pKa = 10.52 AIALAKK133 pKa = 7.96 EE134 pKa = 4.07 HH135 pKa = 7.02 IYY137 pKa = 10.25 IKK139 pKa = 10.74 HH140 pKa = 5.64 EE141 pKa = 4.32 MFHH144 pKa = 8.3 SMAISLRR151 pKa = 11.84 LINASLAMILNTPGMHH167 pKa = 7.32 KK168 pKa = 9.2 YY169 pKa = 8.73 TKK171 pKa = 10.62 LPFYY175 pKa = 10.21 CQLIRR180 pKa = 11.84 DD181 pKa = 4.06 FLKK184 pKa = 11.15 NKK186 pKa = 9.58 ILNNSVTLTGLLLPRR201 pKa = 11.84 KK202 pKa = 8.14 YY203 pKa = 9.01 TIKK206 pKa = 10.47 FFEE209 pKa = 4.23 QTAVTNNVILNAEE222 pKa = 4.11 FRR224 pKa = 11.84 TYY226 pKa = 10.48 IQFIKK231 pKa = 10.84 QMLNNPFIRR240 pKa = 11.84 NSFIDD245 pKa = 3.72 MVFAFYY251 pKa = 9.36 VTAHH255 pKa = 6.73 HH256 pKa = 6.48 FTTDD260 pKa = 2.96 ADD262 pKa = 3.61 ITEE265 pKa = 4.57 SGISDD270 pKa = 3.3 TFEE273 pKa = 4.09 FLNNQYY279 pKa = 10.89 SRR281 pKa = 11.84 FIAEE285 pKa = 3.92 PQIGISVNHH294 pKa = 6.85 TIDD297 pKa = 3.68 GSLNDD302 pKa = 5.62 AINKK306 pKa = 9.33 SLSLFSEE313 pKa = 4.69 LSKK316 pKa = 11.17 ALSDD320 pKa = 4.16 KK321 pKa = 11.13 YY322 pKa = 9.58 QTTVNDD328 pKa = 3.64 YY329 pKa = 8.83 QRR331 pKa = 11.84 KK332 pKa = 8.47 IIASITSIYY341 pKa = 10.32 NLYY344 pKa = 10.65 RR345 pKa = 11.84 FTTHH349 pKa = 7.41 KK350 pKa = 10.79 LSLQDD355 pKa = 3.66 FCVNLCGVIGAAGLASDD372 pKa = 5.64 IITQLIITVRR382 pKa = 11.84 SLFIMPIAQTLSACNFNIIKK402 pKa = 10.49 SIFLSLYY409 pKa = 9.77 CIFVGTLPGKK419 pKa = 8.53 HH420 pKa = 5.38 TVDD423 pKa = 3.54 EE424 pKa = 4.66 FALRR428 pKa = 11.84 MDD430 pKa = 5.34 RR431 pKa = 11.84 FPKK434 pKa = 9.63 MISGIEE440 pKa = 4.18 TMWSKK445 pKa = 11.52 LDD447 pKa = 3.72 LVVGQAHH454 pKa = 5.91 SFIEE458 pKa = 4.4 EE459 pKa = 4.07 KK460 pKa = 10.8 FLGRR464 pKa = 11.84 NNKK467 pKa = 8.85 FVSSEE472 pKa = 3.91 MLDD475 pKa = 3.85 EE476 pKa = 4.15 VTAWADD482 pKa = 3.94 DD483 pKa = 3.49 VATYY487 pKa = 10.47 AGYY490 pKa = 8.67 MEE492 pKa = 4.89 RR493 pKa = 11.84 NEE495 pKa = 4.0 INRR498 pKa = 11.84 DD499 pKa = 3.25 IEE501 pKa = 4.4 TMTAASKK508 pKa = 10.33 LYY510 pKa = 9.54 PRR512 pKa = 11.84 GVRR515 pKa = 11.84 LIKK518 pKa = 10.33 EE519 pKa = 4.16 CTRR522 pKa = 11.84 LKK524 pKa = 10.48 LAPANLNLIRR534 pKa = 11.84 SLLPGAIKK542 pKa = 10.76 LSDD545 pKa = 3.28 AAFKK549 pKa = 10.95 SGANKK554 pKa = 9.92 HH555 pKa = 5.11 SLRR558 pKa = 11.84 VEE560 pKa = 4.58 PIVAWFTGSTGLGKK574 pKa = 8.83 TGMTYY579 pKa = 10.75 PFIIDD584 pKa = 3.34 MMRR587 pKa = 11.84 VFGPVPADD595 pKa = 3.21 WQQNIHH601 pKa = 6.32 ARR603 pKa = 11.84 IAEE606 pKa = 3.86 NEE608 pKa = 4.06 YY609 pKa = 9.98 WDD611 pKa = 5.24 GYY613 pKa = 11.32 DD614 pKa = 3.38 DD615 pKa = 4.84 QEE617 pKa = 4.15 YY618 pKa = 10.76 LIYY621 pKa = 10.7 DD622 pKa = 3.98 DD623 pKa = 6.02 FLQKK627 pKa = 10.06 KK628 pKa = 10.21 DD629 pKa = 3.26 SAANPNVEE637 pKa = 4.58 LFEE640 pKa = 4.66 MIRR643 pKa = 11.84 VTNAFPFQLHH653 pKa = 5.59 MSSVEE658 pKa = 3.98 DD659 pKa = 4.49 KK660 pKa = 11.43 SNKK663 pKa = 8.7 FCNAKK668 pKa = 10.13 FVFLSSNLDD677 pKa = 3.67 VIKK680 pKa = 9.96 TEE682 pKa = 4.4 SLNCPEE688 pKa = 3.87 AVQRR692 pKa = 11.84 RR693 pKa = 11.84 IDD695 pKa = 3.3 YY696 pKa = 10.19 AYY698 pKa = 9.84 RR699 pKa = 11.84 VSIKK703 pKa = 10.46 PEE705 pKa = 3.32 FRR707 pKa = 11.84 EE708 pKa = 4.3 YY709 pKa = 10.85 YY710 pKa = 10.59 VNASGQQCFKK720 pKa = 11.03 LDD722 pKa = 3.38 AAKK725 pKa = 10.48 ARR727 pKa = 11.84 SAARR731 pKa = 11.84 ILLGPNKK738 pKa = 10.26 KK739 pKa = 9.96 NLTTNNLDD747 pKa = 3.16 VYY749 pKa = 10.06 IFEE752 pKa = 4.67 RR753 pKa = 11.84 FSVFDD758 pKa = 3.88 GKK760 pKa = 8.46 TLQTNMSYY768 pKa = 11.18 KK769 pKa = 10.55 DD770 pKa = 3.55 VVEE773 pKa = 4.32 QCSSALEE780 pKa = 3.95 NRR782 pKa = 11.84 FARR785 pKa = 11.84 HH786 pKa = 5.22 VDD788 pKa = 3.04 SAEE791 pKa = 3.72 YY792 pKa = 10.78 LEE794 pKa = 5.63 AYY796 pKa = 9.34 RR797 pKa = 11.84 NPQWLDD803 pKa = 3.39 SIPPPNYY810 pKa = 8.92 EE811 pKa = 3.7 ASLDD815 pKa = 3.58 IVEE818 pKa = 5.38 FPLQASAEE826 pKa = 4.19 IGVGSATAVIAARR839 pKa = 11.84 MSRR842 pKa = 11.84 ILILQHH848 pKa = 6.57 FINAFFFGNRR858 pKa = 11.84 EE859 pKa = 3.93 SALMDD864 pKa = 3.53 FLYY867 pKa = 10.56 AIKK870 pKa = 10.41 HH871 pKa = 4.97 GMSKK875 pKa = 9.69 ILEE878 pKa = 4.29 KK879 pKa = 10.93 FNIIPRR885 pKa = 11.84 SSSIWEE891 pKa = 4.08 NEE893 pKa = 3.89 SFSYY897 pKa = 8.1 YY898 pKa = 10.3 QNYY901 pKa = 9.47 ALSVKK906 pKa = 10.47 NYY908 pKa = 10.5 LSNCKK913 pKa = 8.9 QQLTDD918 pKa = 3.81 TLVSTLGAGWKK929 pKa = 9.11 YY930 pKa = 10.8 FKK932 pKa = 10.34 IALLAGIFILTTMFVKK948 pKa = 10.48 SVEE951 pKa = 4.08 RR952 pKa = 11.84 KK953 pKa = 9.2 YY954 pKa = 10.86 LPKK957 pKa = 10.46 FISEE961 pKa = 4.17 DD962 pKa = 3.31 EE963 pKa = 4.23 KK964 pKa = 11.48 LSVLVKK970 pKa = 10.18 EE971 pKa = 5.56 ANTCLDD977 pKa = 3.68 NDD979 pKa = 3.81 CRR981 pKa = 11.84 NCKK984 pKa = 8.54 TCSNSVSTPLNAKK997 pKa = 8.82 WNSNCACYY1005 pKa = 9.75 IRR1007 pKa = 11.84 RR1008 pKa = 11.84 MEE1010 pKa = 3.97 VSRR1013 pKa = 11.84 DD1014 pKa = 3.45 NIRR1017 pKa = 11.84 QYY1019 pKa = 10.74 CVSMYY1024 pKa = 10.55 GNQRR1028 pKa = 11.84 VSPDD1032 pKa = 3.09 HH1033 pKa = 6.93 EE1034 pKa = 4.35 MTLTDD1039 pKa = 3.63 MYY1041 pKa = 11.6 DD1042 pKa = 3.6 VIEE1045 pKa = 4.36 QICNCDD1051 pKa = 3.12 CSVCHH1056 pKa = 5.67 YY1057 pKa = 11.2 CNDD1060 pKa = 3.65 EE1061 pKa = 4.03 EE1062 pKa = 4.7 LKK1064 pKa = 11.12 SKK1066 pKa = 10.73 LFEE1069 pKa = 3.89 TAKK1072 pKa = 10.34 LHH1074 pKa = 5.94 KK1075 pKa = 8.72 TNCVCLLTRR1084 pKa = 11.84 FYY1086 pKa = 11.07 QGFRR1090 pKa = 11.84 TEE1092 pKa = 4.51 SLLQFLLTLKK1102 pKa = 10.77 DD1103 pKa = 3.64 NLPAYY1108 pKa = 9.41 SLKK1111 pKa = 10.69 NKK1113 pKa = 9.6 EE1114 pKa = 4.47 LIRR1117 pKa = 11.84 LISQHH1122 pKa = 6.29 GIKK1125 pKa = 10.2 DD1126 pKa = 3.53 IPVLPEE1132 pKa = 3.6 FSQNLTQGPLYY1143 pKa = 10.16 DD1144 pKa = 4.34 AKK1146 pKa = 9.43 TKK1148 pKa = 10.65 SVAPITKK1155 pKa = 9.59 IVNQAPAIYY1164 pKa = 9.87 EE1165 pKa = 3.89 GRR1167 pKa = 11.84 QARR1170 pKa = 11.84 NAIRR1174 pKa = 11.84 TVIKK1178 pKa = 10.48 NQAPHH1183 pKa = 6.52 YY1184 pKa = 7.6 DD1185 pKa = 2.98 AKK1187 pKa = 9.49 MKK1189 pKa = 10.48 HH1190 pKa = 5.33 VATSRR1195 pKa = 11.84 IVNQSVANLTPFVDD1209 pKa = 4.31 DD1210 pKa = 3.31 VRR1212 pKa = 11.84 TIISKK1217 pKa = 10.88 ADD1219 pKa = 2.91 IHH1221 pKa = 6.74 VRR1223 pKa = 11.84 NACTISNCGRR1233 pKa = 11.84 CLSEE1237 pKa = 3.88 QATPSLQRR1245 pKa = 11.84 NLPEE1249 pKa = 3.87 QDD1251 pKa = 2.87 VGAITIVRR1259 pKa = 11.84 DD1260 pKa = 3.66 VVYY1263 pKa = 10.9 KK1264 pKa = 10.82 NLFKK1268 pKa = 10.85 FVVTKK1273 pKa = 10.29 TDD1275 pKa = 3.11 SGNVKK1280 pKa = 7.54 TTTYY1284 pKa = 9.91 YY1285 pKa = 11.15 GQIFMLGGRR1294 pKa = 11.84 LGLIPKK1300 pKa = 9.85 HH1301 pKa = 5.72 FLRR1304 pKa = 11.84 AIKK1307 pKa = 9.52 MDD1309 pKa = 3.85 LEE1311 pKa = 4.87 LGFSLEE1317 pKa = 4.12 FCLEE1321 pKa = 3.81 DD1322 pKa = 3.34 AFAVITSQYY1331 pKa = 8.58 PVEE1334 pKa = 5.88 IILDD1338 pKa = 3.78 AEE1340 pKa = 4.0 NHH1342 pKa = 6.19 IEE1344 pKa = 4.21 HH1345 pKa = 7.77 DD1346 pKa = 4.06 SRR1348 pKa = 11.84 DD1349 pKa = 3.45 LAIIQLPINAGCYY1362 pKa = 8.95 SQAFKK1367 pKa = 10.81 HH1368 pKa = 6.45 IVDD1371 pKa = 3.84 EE1372 pKa = 4.07 QDD1374 pKa = 3.31 LFRR1377 pKa = 11.84 VGHH1380 pKa = 6.09 NPGILARR1387 pKa = 11.84 YY1388 pKa = 5.76 QTATEE1393 pKa = 3.92 KK1394 pKa = 10.99 DD1395 pKa = 3.21 RR1396 pKa = 11.84 LKK1398 pKa = 11.02 GIRR1401 pKa = 11.84 HH1402 pKa = 4.85 YY1403 pKa = 11.33 RR1404 pKa = 11.84 EE1405 pKa = 3.49 MFYY1408 pKa = 11.02 LSTLTPEE1415 pKa = 4.67 DD1416 pKa = 3.83 SLVEE1420 pKa = 3.96 TNMRR1424 pKa = 11.84 DD1425 pKa = 3.5 EE1426 pKa = 5.21 IITNRR1431 pKa = 11.84 GSYY1434 pKa = 9.87 LYY1436 pKa = 10.47 HH1437 pKa = 6.78 AVTVPGDD1444 pKa = 3.71 CGSVLVARR1452 pKa = 11.84 STSITQKK1459 pKa = 10.26 IVGIHH1464 pKa = 5.79 IAGLMGVVEE1473 pKa = 5.24 GISVSITQQMIVKK1486 pKa = 9.11 MMSHH1490 pKa = 7.27 FKK1492 pKa = 10.74 SSSQYY1497 pKa = 8.57 GHH1499 pKa = 6.76 AVVPFDD1505 pKa = 3.6 VRR1507 pKa = 11.84 SDD1509 pKa = 3.18 ILRR1512 pKa = 11.84 EE1513 pKa = 3.83 NGTFQLHH1520 pKa = 4.79 GTKK1523 pKa = 10.36 VGVRR1527 pKa = 11.84 INGSVKK1533 pKa = 9.24 TAMSRR1538 pKa = 11.84 SAAFGALCVSPNKK1551 pKa = 9.64 PGHH1554 pKa = 6.41 LRR1556 pKa = 11.84 PFVNPQGEE1564 pKa = 4.51 RR1565 pKa = 11.84 IDD1567 pKa = 3.65 PMKK1570 pKa = 10.52 LQRR1573 pKa = 11.84 SKK1575 pKa = 11.33 YY1576 pKa = 8.35 GVVRR1580 pKa = 11.84 PFVTFSRR1587 pKa = 11.84 VQTVYY1592 pKa = 10.55 EE1593 pKa = 4.02 AMTVFYY1599 pKa = 10.57 HH1600 pKa = 7.53 RR1601 pKa = 11.84 EE1602 pKa = 3.94 YY1603 pKa = 11.06 QNTPEE1608 pKa = 4.41 WYY1610 pKa = 9.12 KK1611 pKa = 10.92 QPLTLEE1617 pKa = 4.01 EE1618 pKa = 4.77 AIIGIDD1624 pKa = 3.43 GDD1626 pKa = 4.04 PFINAINRR1634 pKa = 11.84 QTAPGYY1640 pKa = 9.73 PYY1642 pKa = 10.57 TLNKK1646 pKa = 9.95 PKK1648 pKa = 10.01 GTVGKK1653 pKa = 10.17 QGWFGKK1659 pKa = 9.62 EE1660 pKa = 3.5 MEE1662 pKa = 4.18 YY1663 pKa = 11.25 DD1664 pKa = 3.55 LTNSHH1669 pKa = 6.95 CLQLLDD1675 pKa = 5.63 DD1676 pKa = 4.38 VEE1678 pKa = 4.18 QLKK1681 pKa = 11.13 LSMLEE1686 pKa = 3.77 NVRR1689 pKa = 11.84 PEE1691 pKa = 4.43 VIWIDD1696 pKa = 3.58 TLKK1699 pKa = 10.73 DD1700 pKa = 3.37 AKK1702 pKa = 10.69 IPIAKK1707 pKa = 9.89 ADD1709 pKa = 3.29 IGKK1712 pKa = 8.11 TRR1714 pKa = 11.84 LFTACPMHH1722 pKa = 6.35 YY1723 pKa = 10.25 SIAFRR1728 pKa = 11.84 QYY1730 pKa = 8.13 FLPFIAHH1737 pKa = 7.01 AMRR1740 pKa = 11.84 NRR1742 pKa = 11.84 VDD1744 pKa = 3.07 NSLAVGINPTSVEE1757 pKa = 4.05 WTKK1760 pKa = 10.61 LAQRR1764 pKa = 11.84 LQRR1767 pKa = 11.84 QGSNVIAGDD1776 pKa = 3.54 YY1777 pKa = 11.07 SNFDD1781 pKa = 2.98 GTLPVQYY1788 pKa = 10.89 VEE1790 pKa = 4.22 VAVKK1794 pKa = 10.28 IMCDD1798 pKa = 2.91 WLRR1801 pKa = 11.84 VNWEE1805 pKa = 4.29 NIVKK1809 pKa = 10.44 AKK1811 pKa = 10.57 RR1812 pKa = 11.84 NVVCGRR1818 pKa = 11.84 NLTEE1822 pKa = 3.7 EE1823 pKa = 3.81 QFYY1826 pKa = 11.47 DD1827 pKa = 3.71 FLYY1830 pKa = 10.66 KK1831 pKa = 10.68 LGMEE1835 pKa = 5.12 CFNHH1839 pKa = 6.03 LHH1841 pKa = 6.3 IANHH1845 pKa = 6.14 EE1846 pKa = 4.12 EE1847 pKa = 3.94 AKK1849 pKa = 10.53 GALVYY1854 pKa = 8.81 FVRR1857 pKa = 11.84 NGIPSGCPATAILNSIVNHH1876 pKa = 6.11 CVLADD1881 pKa = 3.33 SWLSIMQNEE1890 pKa = 4.15 PLYY1893 pKa = 10.93 EE1894 pKa = 4.25 NLATMSAFFEE1904 pKa = 4.43 HH1905 pKa = 6.74 TSSIFYY1911 pKa = 10.87 GDD1913 pKa = 3.95 DD1914 pKa = 3.64 FIMNIRR1920 pKa = 11.84 HH1921 pKa = 5.71 SVIDD1925 pKa = 4.52 LYY1927 pKa = 11.41 NQEE1930 pKa = 4.45 TLTQVLKK1937 pKa = 10.33 TNLDD1941 pKa = 3.07 MDD1943 pKa = 3.96 MTDD1946 pKa = 3.21 EE1947 pKa = 4.81 AKK1949 pKa = 10.31 TGDD1952 pKa = 3.21 IVKK1955 pKa = 10.09 ARR1957 pKa = 11.84 KK1958 pKa = 8.8 LADD1961 pKa = 3.16 VSFLKK1966 pKa = 10.56 RR1967 pKa = 11.84 KK1968 pKa = 9.17 FRR1970 pKa = 11.84 FEE1972 pKa = 3.91 EE1973 pKa = 4.86 SIQLWVSPIDD1983 pKa = 3.66 INVLLDD1989 pKa = 3.37 APNWVRR1995 pKa = 11.84 AGNASALQICVDD2007 pKa = 3.92 TLSTYY2012 pKa = 10.94 CLTEE2016 pKa = 4.02 LALHH2020 pKa = 6.7 DD2021 pKa = 5.12 RR2022 pKa = 11.84 DD2023 pKa = 3.91 VDD2025 pKa = 4.24 DD2026 pKa = 3.44 YY2027 pKa = 11.08 WRR2029 pKa = 11.84 PKK2031 pKa = 9.69 MVACGLNITRR2041 pKa = 11.84 GTRR2044 pKa = 11.84 IVFNPDD2050 pKa = 2.28 NRR2052 pKa = 11.84 RR2053 pKa = 11.84 SVLAKK2058 pKa = 10.17 FRR2060 pKa = 11.84 NEE2062 pKa = 3.86 QLNTEE2067 pKa = 4.2 INFF2070 pKa = 3.68
Molecular weight: 235.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.896
IPC2_protein 7.249
IPC_protein 7.044
Toseland 6.839
ProMoST 7.658
Dawson 7.732
Bjellqvist 8.17
Wikipedia 7.644
Rodwell 7.761
Grimsley 6.927
Solomon 7.746
Lehninger 7.761
Nozaki 8.361
DTASelect 7.907
Thurlkill 7.966
EMBOSS 7.966
Sillero 8.273
Patrickios 4.126
IPC_peptide 7.746
IPC2_peptide 7.293
IPC2.peptide.svr19 7.183
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2843
773
2070
1421.5
160.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.598 ± 0.683
1.653 ± 0.584
5.135 ± 0.113
4.643 ± 0.658
5.276 ± 0.281
4.784 ± 0.6
2.005 ± 0.499
7.281 ± 0.551
4.713 ± 1.227
8.864 ± 0.766
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.353
5.628 ± 0.899
4.326 ± 0.902
3.975 ± 0.115
5.171 ± 0.002
7.633 ± 1.198
6.824 ± 1.148
6.789 ± 0.501
0.95 ± 0.044
4.256 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here