Cotton leaf curl Alabad virus-[802a]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Cotton leaf curl Alabad virus

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q77CF0|Q77CF0_9GEMI Protein V2 OS=Cotton leaf curl Alabad virus-[802a] OX=222457 GN=AV2 PE=3 SV=1
MM1 pKa = 7.59PRR3 pKa = 11.84NNSFCINAKK12 pKa = 10.45NIFLTFPKK20 pKa = 10.37CPIPKK25 pKa = 9.7EE26 pKa = 3.87QMLDD30 pKa = 2.9ILSNISCPSDD40 pKa = 3.09KK41 pKa = 11.04LFIRR45 pKa = 11.84VAQEE49 pKa = 3.46KK50 pKa = 9.49HH51 pKa = 5.19QDD53 pKa = 3.38GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.83GKK67 pKa = 10.55AKK69 pKa = 9.97FRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 5.97FDD77 pKa = 2.95VTHH80 pKa = 6.8PHH82 pKa = 6.82TSTQFHH88 pKa = 7.33PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.55KK106 pKa = 10.86DD107 pKa = 2.8GDD109 pKa = 3.98YY110 pKa = 10.51IDD112 pKa = 4.32WGTFQIDD119 pKa = 3.03GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 8.46EE144 pKa = 3.51AAMAIIRR151 pKa = 11.84EE152 pKa = 4.04KK153 pKa = 10.92LPEE156 pKa = 3.95KK157 pKa = 10.64FIFQYY162 pKa = 11.22HH163 pKa = 5.88NLNSNLDD170 pKa = 4.12RR171 pKa = 11.84IFTPPLEE178 pKa = 4.56VYY180 pKa = 10.35VSPFLSSSFNQVPEE194 pKa = 4.2EE195 pKa = 3.99LEE197 pKa = 3.93EE198 pKa = 4.22WVSEE202 pKa = 4.09NVMDD206 pKa = 5.26AAARR210 pKa = 11.84PLRR213 pKa = 11.84PQSIVIEE220 pKa = 4.44GDD222 pKa = 3.12SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 4.79DD262 pKa = 4.33VDD264 pKa = 3.73PHH266 pKa = 6.08FLKK269 pKa = 10.67HH270 pKa = 5.9FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.07KK312 pKa = 10.49EE313 pKa = 3.93YY314 pKa = 10.96LDD316 pKa = 3.83EE317 pKa = 5.21EE318 pKa = 4.63KK319 pKa = 11.11NQALKK324 pKa = 10.79NWAVKK329 pKa = 10.09NAVFITLEE337 pKa = 3.94EE338 pKa = 4.03PLYY341 pKa = 10.9SIAHH345 pKa = 5.35QSPAQGGQEE354 pKa = 3.67EE355 pKa = 4.37DD356 pKa = 3.45QEE358 pKa = 4.47EE359 pKa = 4.49EE360 pKa = 4.22SGSS363 pKa = 3.6

Molecular weight:
41.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q77CF1|Q77CF1_9GEMI Capsid protein OS=Cotton leaf curl Alabad virus-[802a] OX=222457 GN=AV1 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.21RR4 pKa = 11.84AADD7 pKa = 3.55IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSPYY27 pKa = 8.67TSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.53VTRR97 pKa = 11.84GVGLTHH103 pKa = 7.8RR104 pKa = 11.84IGKK107 pKa = 8.54RR108 pKa = 11.84FCVKK112 pKa = 9.96SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight:
29.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1109

85

363

184.8

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.869 ± 1.055

2.344 ± 0.67

4.689 ± 0.426

5.05 ± 0.893

4.328 ± 0.595

4.779 ± 0.392

3.877 ± 0.568

5.32 ± 0.665

5.771 ± 0.744

7.214 ± 1.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.976 ± 0.662

6.041 ± 0.584

6.222 ± 0.658

5.23 ± 0.726

6.312 ± 1.113

8.656 ± 1.514

5.23 ± 0.902

6.041 ± 1.416

1.262 ± 0.154

3.787 ± 0.703

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski