Cotton leaf curl Alabad virus-[802a]
Average proteome isoelectric point is 7.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q77CF0|Q77CF0_9GEMI Protein V2 OS=Cotton leaf curl Alabad virus-[802a] OX=222457 GN=AV2 PE=3 SV=1
MM1 pKa = 7.59 PRR3 pKa = 11.84 NNSFCINAKK12 pKa = 10.45 NIFLTFPKK20 pKa = 10.37 CPIPKK25 pKa = 9.7 EE26 pKa = 3.87 QMLDD30 pKa = 2.9 ILSNISCPSDD40 pKa = 3.09 KK41 pKa = 11.04 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.46 KK50 pKa = 9.49 HH51 pKa = 5.19 QDD53 pKa = 3.38 GSLHH57 pKa = 5.16 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.83 GKK67 pKa = 10.55 AKK69 pKa = 9.97 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 5.97 FDD77 pKa = 2.95 VTHH80 pKa = 6.8 PHH82 pKa = 6.82 TSTQFHH88 pKa = 7.33 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.23 SYY103 pKa = 10.8 IEE105 pKa = 4.55 KK106 pKa = 10.86 DD107 pKa = 2.8 GDD109 pKa = 3.98 YY110 pKa = 10.51 IDD112 pKa = 4.32 WGTFQIDD119 pKa = 3.03 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.92 ALNAGSKK143 pKa = 8.46 EE144 pKa = 3.51 AAMAIIRR151 pKa = 11.84 EE152 pKa = 4.04 KK153 pKa = 10.92 LPEE156 pKa = 3.95 KK157 pKa = 10.64 FIFQYY162 pKa = 11.22 HH163 pKa = 5.88 NLNSNLDD170 pKa = 4.12 RR171 pKa = 11.84 IFTPPLEE178 pKa = 4.56 VYY180 pKa = 10.35 VSPFLSSSFNQVPEE194 pKa = 4.2 EE195 pKa = 3.99 LEE197 pKa = 3.93 EE198 pKa = 4.22 WVSEE202 pKa = 4.09 NVMDD206 pKa = 5.26 AAARR210 pKa = 11.84 PLRR213 pKa = 11.84 PQSIVIEE220 pKa = 4.44 GDD222 pKa = 3.12 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 4.79 DD262 pKa = 4.33 VDD264 pKa = 3.73 PHH266 pKa = 6.08 FLKK269 pKa = 10.67 HH270 pKa = 5.9 FKK272 pKa = 10.69 EE273 pKa = 4.43 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPNSSYY311 pKa = 11.07 KK312 pKa = 10.49 EE313 pKa = 3.93 YY314 pKa = 10.96 LDD316 pKa = 3.83 EE317 pKa = 5.21 EE318 pKa = 4.63 KK319 pKa = 11.11 NQALKK324 pKa = 10.79 NWAVKK329 pKa = 10.09 NAVFITLEE337 pKa = 3.94 EE338 pKa = 4.03 PLYY341 pKa = 10.9 SIAHH345 pKa = 5.35 QSPAQGGQEE354 pKa = 3.67 EE355 pKa = 4.37 DD356 pKa = 3.45 QEE358 pKa = 4.47 EE359 pKa = 4.49 EE360 pKa = 4.22 SGSS363 pKa = 3.6
Molecular weight: 41.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.697
IPC2_protein 5.614
IPC_protein 5.652
Toseland 6.084
ProMoST 5.982
Dawson 5.906
Bjellqvist 5.919
Wikipedia 5.906
Rodwell 5.893
Grimsley 6.262
Solomon 5.906
Lehninger 5.893
Nozaki 6.173
DTASelect 6.351
Thurlkill 6.364
EMBOSS 6.326
Sillero 6.262
Patrickios 4.177
IPC_peptide 5.919
IPC2_peptide 6.262
IPC2.peptide.svr19 6.221
Protein with the highest isoelectric point:
>tr|Q77CF1|Q77CF1_9GEMI Capsid protein OS=Cotton leaf curl Alabad virus-[802a] OX=222457 GN=AV1 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.21 RR4 pKa = 11.84 AADD7 pKa = 3.55 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFGSPYY27 pKa = 8.67 TSRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 9.91 QQAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.8 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.8 RR104 pKa = 11.84 IGKK107 pKa = 8.54 RR108 pKa = 11.84 FCVKK112 pKa = 9.96 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.0 RR173 pKa = 11.84 DD174 pKa = 3.31 RR175 pKa = 11.84 YY176 pKa = 8.78 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.58 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.68 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.56
IPC_protein 9.78
Toseland 10.16
ProMoST 9.867
Dawson 10.365
Bjellqvist 10.058
Wikipedia 10.555
Rodwell 10.73
Grimsley 10.438
Solomon 10.394
Lehninger 10.365
Nozaki 10.189
DTASelect 10.043
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.306
IPC_peptide 10.394
IPC2_peptide 8.916
IPC2.peptide.svr19 8.532
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1109
85
363
184.8
21.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.869 ± 1.055
2.344 ± 0.67
4.689 ± 0.426
5.05 ± 0.893
4.328 ± 0.595
4.779 ± 0.392
3.877 ± 0.568
5.32 ± 0.665
5.771 ± 0.744
7.214 ± 1.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.976 ± 0.662
6.041 ± 0.584
6.222 ± 0.658
5.23 ± 0.726
6.312 ± 1.113
8.656 ± 1.514
5.23 ± 0.902
6.041 ± 1.416
1.262 ± 0.154
3.787 ± 0.703
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here