Amino acid dipepetide frequency for Bacillus phage Eldridge

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.855AlaAla: 4.855 ± 0.342
0.49AlaCys: 0.49 ± 0.092
4.365AlaAsp: 4.365 ± 0.288
4.406AlaGlu: 4.406 ± 0.365
2.693AlaPhe: 2.693 ± 0.221
4.875AlaGly: 4.875 ± 0.382
0.775AlaHis: 0.775 ± 0.11
5.079AlaIle: 5.079 ± 0.367
4.304AlaLys: 4.304 ± 0.285
5.487AlaLeu: 5.487 ± 0.385
1.591AlaMet: 1.591 ± 0.188
3.774AlaAsn: 3.774 ± 0.347
2.244AlaPro: 2.244 ± 0.452
2.631AlaGln: 2.631 ± 0.254
2.896AlaArg: 2.896 ± 0.234
3.978AlaSer: 3.978 ± 0.352
3.937AlaThr: 3.937 ± 0.344
4.182AlaVal: 4.182 ± 0.324
0.592AlaTrp: 0.592 ± 0.101
3.06AlaTyr: 3.06 ± 0.26
0.0AlaXaa: 0.0 ± 0.0
Cys
0.326CysAla: 0.326 ± 0.075
0.204CysCys: 0.204 ± 0.064
0.53CysAsp: 0.53 ± 0.115
0.449CysGlu: 0.449 ± 0.09
0.326CysPhe: 0.326 ± 0.086
0.51CysGly: 0.51 ± 0.132
0.265CysHis: 0.265 ± 0.073
0.326CysIle: 0.326 ± 0.082
0.755CysLys: 0.755 ± 0.144
0.694CysLeu: 0.694 ± 0.131
0.204CysMet: 0.204 ± 0.062
0.551CysAsn: 0.551 ± 0.101
0.51CysPro: 0.51 ± 0.118
0.184CysGln: 0.184 ± 0.059
0.306CysArg: 0.306 ± 0.07
0.53CysSer: 0.53 ± 0.104
0.632CysThr: 0.632 ± 0.112
0.632CysVal: 0.632 ± 0.102
0.102CysTrp: 0.102 ± 0.038
0.204CysTyr: 0.204 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
4.182AspAla: 4.182 ± 0.32
0.592AspCys: 0.592 ± 0.116
2.978AspAsp: 2.978 ± 0.285
4.386AspGlu: 4.386 ± 0.385
2.876AspPhe: 2.876 ± 0.24
4.834AspGly: 4.834 ± 0.292
0.898AspHis: 0.898 ± 0.139
4.447AspIle: 4.447 ± 0.327
4.916AspLys: 4.916 ± 0.383
4.916AspLeu: 4.916 ± 0.331
2.04AspMet: 2.04 ± 0.18
3.508AspAsn: 3.508 ± 0.283
2.162AspPro: 2.162 ± 0.223
1.877AspGln: 1.877 ± 0.17
2.774AspArg: 2.774 ± 0.271
3.427AspSer: 3.427 ± 0.24
4.141AspThr: 4.141 ± 0.345
4.528AspVal: 4.528 ± 0.275
0.898AspTrp: 0.898 ± 0.134
2.876AspTyr: 2.876 ± 0.235
0.0AspXaa: 0.0 ± 0.0
Glu
4.732GluAla: 4.732 ± 0.322
0.551GluCys: 0.551 ± 0.108
4.957GluAsp: 4.957 ± 0.357
7.71GluGlu: 7.71 ± 0.647
2.631GluPhe: 2.631 ± 0.27
4.63GluGly: 4.63 ± 0.347
1.244GluHis: 1.244 ± 0.205
4.793GluIle: 4.793 ± 0.312
5.487GluLys: 5.487 ± 0.49
6.935GluLeu: 6.935 ± 0.406
1.999GluMet: 1.999 ± 0.193
3.712GluAsn: 3.712 ± 0.263
1.938GluPro: 1.938 ± 0.224
2.733GluGln: 2.733 ± 0.292
2.754GluArg: 2.754 ± 0.231
3.243GluSer: 3.243 ± 0.272
3.447GluThr: 3.447 ± 0.315
5.813GluVal: 5.813 ± 0.359
0.959GluTrp: 0.959 ± 0.135
2.733GluTyr: 2.733 ± 0.23
0.0GluXaa: 0.0 ± 0.0
Phe
2.06PheAla: 2.06 ± 0.202
0.428PheCys: 0.428 ± 0.099
2.958PheAsp: 2.958 ± 0.252
2.652PheGlu: 2.652 ± 0.224
1.469PhePhe: 1.469 ± 0.177
2.529PheGly: 2.529 ± 0.248
0.796PheHis: 0.796 ± 0.153
2.937PheIle: 2.937 ± 0.227
2.835PheLys: 2.835 ± 0.241
3.223PheLeu: 3.223 ± 0.253
0.979PheMet: 0.979 ± 0.157
2.713PheAsn: 2.713 ± 0.276
1.448PhePro: 1.448 ± 0.197
1.265PheGln: 1.265 ± 0.189
1.367PheArg: 1.367 ± 0.164
3.264PheSer: 3.264 ± 0.294
3.264PheThr: 3.264 ± 0.243
2.631PheVal: 2.631 ± 0.218
0.245PheTrp: 0.245 ± 0.07
1.754PheTyr: 1.754 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
4.406GlyAla: 4.406 ± 0.39
0.571GlyCys: 0.571 ± 0.122
4.08GlyAsp: 4.08 ± 0.285
3.916GlyGlu: 3.916 ± 0.29
2.998GlyPhe: 2.998 ± 0.252
5.752GlyGly: 5.752 ± 0.776
0.959GlyHis: 0.959 ± 0.163
4.08GlyIle: 4.08 ± 0.292
4.671GlyLys: 4.671 ± 0.3
5.609GlyLeu: 5.609 ± 0.321
1.775GlyMet: 1.775 ± 0.196
4.018GlyAsn: 4.018 ± 0.289
0.673GlyPro: 0.673 ± 0.101
2.305GlyGln: 2.305 ± 0.213
2.611GlyArg: 2.611 ± 0.249
4.855GlySer: 4.855 ± 0.394
4.793GlyThr: 4.793 ± 0.431
5.263GlyVal: 5.263 ± 0.333
1.04GlyTrp: 1.04 ± 0.163
3.508GlyTyr: 3.508 ± 0.294
0.0GlyXaa: 0.0 ± 0.0
His
1.02HisAla: 1.02 ± 0.151
0.224HisCys: 0.224 ± 0.069
1.061HisAsp: 1.061 ± 0.161
0.938HisGlu: 0.938 ± 0.141
0.796HisPhe: 0.796 ± 0.132
0.979HisGly: 0.979 ± 0.126
0.367HisHis: 0.367 ± 0.095
1.224HisIle: 1.224 ± 0.165
1.571HisLys: 1.571 ± 0.177
1.591HisLeu: 1.591 ± 0.192
0.408HisMet: 0.408 ± 0.102
1.55HisAsn: 1.55 ± 0.209
0.551HisPro: 0.551 ± 0.098
0.306HisGln: 0.306 ± 0.074
1.142HisArg: 1.142 ± 0.142
0.959HisSer: 0.959 ± 0.179
1.183HisThr: 1.183 ± 0.14
1.407HisVal: 1.407 ± 0.169
0.061HisTrp: 0.061 ± 0.055
0.734HisTyr: 0.734 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.243IleAla: 4.243 ± 0.259
0.551IleCys: 0.551 ± 0.102
5.446IleAsp: 5.446 ± 0.348
5.773IleGlu: 5.773 ± 0.367
2.142IlePhe: 2.142 ± 0.217
3.835IleGly: 3.835 ± 0.342
1.244IleHis: 1.244 ± 0.164
4.141IleIle: 4.141 ± 0.332
4.916IleLys: 4.916 ± 0.318
4.182IleLeu: 4.182 ± 0.391
1.448IleMet: 1.448 ± 0.163
3.774IleAsn: 3.774 ± 0.33
2.305IlePro: 2.305 ± 0.227
3.06IleGln: 3.06 ± 0.249
3.08IleArg: 3.08 ± 0.257
3.529IleSer: 3.529 ± 0.303
4.528IleThr: 4.528 ± 0.332
3.916IleVal: 3.916 ± 0.296
0.449IleTrp: 0.449 ± 0.095
2.019IleTyr: 2.019 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
5.161LysAla: 5.161 ± 0.332
0.469LysCys: 0.469 ± 0.106
4.488LysAsp: 4.488 ± 0.305
6.996LysGlu: 6.996 ± 0.496
2.427LysPhe: 2.427 ± 0.218
4.916LysGly: 4.916 ± 0.419
1.305LysHis: 1.305 ± 0.188
3.978LysIle: 3.978 ± 0.37
5.671LysLys: 5.671 ± 0.448
6.242LysLeu: 6.242 ± 0.268
2.57LysMet: 2.57 ± 0.222
2.978LysAsn: 2.978 ± 0.27
2.693LysPro: 2.693 ± 0.275
3.345LysGln: 3.345 ± 0.252
3.447LysArg: 3.447 ± 0.302
4.222LysSer: 4.222 ± 0.358
3.631LysThr: 3.631 ± 0.269
4.855LysVal: 4.855 ± 0.344
0.612LysTrp: 0.612 ± 0.106
3.182LysTyr: 3.182 ± 0.225
0.0LysXaa: 0.0 ± 0.0
Leu
5.813LeuAla: 5.813 ± 0.343
0.469LeuCys: 0.469 ± 0.11
5.671LeuAsp: 5.671 ± 0.327
5.936LeuGlu: 5.936 ± 0.362
3.937LeuPhe: 3.937 ± 0.278
4.977LeuGly: 4.977 ± 0.358
1.775LeuHis: 1.775 ± 0.182
4.488LeuIle: 4.488 ± 0.283
6.16LeuLys: 6.16 ± 0.343
5.732LeuLeu: 5.732 ± 0.423
1.938LeuMet: 1.938 ± 0.185
4.549LeuAsn: 4.549 ± 0.289
2.998LeuPro: 2.998 ± 0.317
3.182LeuGln: 3.182 ± 0.254
3.202LeuArg: 3.202 ± 0.262
5.059LeuSer: 5.059 ± 0.3
5.752LeuThr: 5.752 ± 0.364
5.059LeuVal: 5.059 ± 0.344
0.653LeuTrp: 0.653 ± 0.118
3.468LeuTyr: 3.468 ± 0.257
0.0LeuXaa: 0.0 ± 0.0
Met
1.815MetAla: 1.815 ± 0.152
0.224MetCys: 0.224 ± 0.065
1.367MetAsp: 1.367 ± 0.163
1.999MetGlu: 1.999 ± 0.234
1.183MetPhe: 1.183 ± 0.159
1.448MetGly: 1.448 ± 0.162
0.408MetHis: 0.408 ± 0.078
1.754MetIle: 1.754 ± 0.196
2.203MetLys: 2.203 ± 0.229
2.346MetLeu: 2.346 ± 0.23
0.632MetMet: 0.632 ± 0.124
1.346MetAsn: 1.346 ± 0.16
0.592MetPro: 0.592 ± 0.104
0.938MetGln: 0.938 ± 0.16
1.387MetArg: 1.387 ± 0.16
2.081MetSer: 2.081 ± 0.217
1.897MetThr: 1.897 ± 0.21
1.53MetVal: 1.53 ± 0.204
0.143MetTrp: 0.143 ± 0.056
1.265MetTyr: 1.265 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.121AsnAla: 3.121 ± 0.276
0.51AsnCys: 0.51 ± 0.123
2.794AsnAsp: 2.794 ± 0.235
3.304AsnGlu: 3.304 ± 0.304
1.938AsnPhe: 1.938 ± 0.246
4.508AsnGly: 4.508 ± 0.358
1.183AsnHis: 1.183 ± 0.166
3.876AsnIle: 3.876 ± 0.388
4.202AsnLys: 4.202 ± 0.278
4.508AsnLeu: 4.508 ± 0.339
1.632AsnMet: 1.632 ± 0.18
2.958AsnAsn: 2.958 ± 0.29
2.244AsnPro: 2.244 ± 0.261
1.836AsnGln: 1.836 ± 0.241
2.448AsnArg: 2.448 ± 0.208
3.59AsnSer: 3.59 ± 0.367
4.141AsnThr: 4.141 ± 0.324
3.631AsnVal: 3.631 ± 0.31
0.51AsnTrp: 0.51 ± 0.092
2.713AsnTyr: 2.713 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
2.489ProAla: 2.489 ± 0.326
0.449ProCys: 0.449 ± 0.115
2.264ProAsp: 2.264 ± 0.188
2.285ProGlu: 2.285 ± 0.221
1.122ProPhe: 1.122 ± 0.148
1.101ProGly: 1.101 ± 0.166
0.571ProHis: 0.571 ± 0.1
2.019ProIle: 2.019 ± 0.207
2.856ProLys: 2.856 ± 0.25
2.244ProLeu: 2.244 ± 0.259
0.755ProMet: 0.755 ± 0.142
1.917ProAsn: 1.917 ± 0.239
0.979ProPro: 0.979 ± 0.186
1.203ProGln: 1.203 ± 0.198
1.142ProArg: 1.142 ± 0.193
2.264ProSer: 2.264 ± 0.245
2.713ProThr: 2.713 ± 0.263
2.203ProVal: 2.203 ± 0.291
0.245ProTrp: 0.245 ± 0.07
1.571ProTyr: 1.571 ± 0.188
0.0ProXaa: 0.0 ± 0.0
Gln
3.264GlnAla: 3.264 ± 0.28
0.265GlnCys: 0.265 ± 0.068
2.142GlnAsp: 2.142 ± 0.207
3.08GlnGlu: 3.08 ± 0.298
1.591GlnPhe: 1.591 ± 0.184
2.305GlnGly: 2.305 ± 0.191
0.734GlnHis: 0.734 ± 0.124
1.917GlnIle: 1.917 ± 0.228
1.999GlnLys: 1.999 ± 0.203
3.304GlnLeu: 3.304 ± 0.29
1.183GlnMet: 1.183 ± 0.197
1.856GlnAsn: 1.856 ± 0.219
1.122GlnPro: 1.122 ± 0.191
1.979GlnGln: 1.979 ± 0.278
1.693GlnArg: 1.693 ± 0.183
2.183GlnSer: 2.183 ± 0.251
1.979GlnThr: 1.979 ± 0.172
2.366GlnVal: 2.366 ± 0.198
0.51GlnTrp: 0.51 ± 0.096
1.428GlnTyr: 1.428 ± 0.169
0.0GlnXaa: 0.0 ± 0.0
Arg
2.55ArgAla: 2.55 ± 0.212
0.469ArgCys: 0.469 ± 0.115
2.835ArgAsp: 2.835 ± 0.275
2.998ArgGlu: 2.998 ± 0.274
1.897ArgPhe: 1.897 ± 0.165
2.937ArgGly: 2.937 ± 0.298
0.857ArgHis: 0.857 ± 0.11
3.019ArgIle: 3.019 ± 0.234
2.835ArgLys: 2.835 ± 0.206
3.733ArgLeu: 3.733 ± 0.24
1.509ArgMet: 1.509 ± 0.152
2.121ArgAsn: 2.121 ± 0.223
1.326ArgPro: 1.326 ± 0.169
1.387ArgGln: 1.387 ± 0.16
1.734ArgArg: 1.734 ± 0.192
2.142ArgSer: 2.142 ± 0.237
2.815ArgThr: 2.815 ± 0.287
2.978ArgVal: 2.978 ± 0.256
0.49ArgTrp: 0.49 ± 0.1
1.754ArgTyr: 1.754 ± 0.178
0.0ArgXaa: 0.0 ± 0.0
Ser
3.712SerAla: 3.712 ± 0.254
0.367SerCys: 0.367 ± 0.103
3.366SerAsp: 3.366 ± 0.253
4.059SerGlu: 4.059 ± 0.35
3.121SerPhe: 3.121 ± 0.269
5.099SerGly: 5.099 ± 0.481
1.02SerHis: 1.02 ± 0.156
4.202SerIle: 4.202 ± 0.298
4.263SerLys: 4.263 ± 0.264
4.732SerLeu: 4.732 ± 0.289
1.509SerMet: 1.509 ± 0.207
3.427SerAsn: 3.427 ± 0.257
1.693SerPro: 1.693 ± 0.195
1.999SerGln: 1.999 ± 0.184
2.754SerArg: 2.754 ± 0.228
4.141SerSer: 4.141 ± 0.363
3.896SerThr: 3.896 ± 0.412
4.324SerVal: 4.324 ± 0.353
0.734SerTrp: 0.734 ± 0.13
2.754SerTyr: 2.754 ± 0.225
0.0SerXaa: 0.0 ± 0.0
Thr
4.488ThrAla: 4.488 ± 0.372
0.449ThrCys: 0.449 ± 0.087
4.263ThrAsp: 4.263 ± 0.321
3.712ThrGlu: 3.712 ± 0.251
2.794ThrPhe: 2.794 ± 0.264
4.957ThrGly: 4.957 ± 0.334
1.224ThrHis: 1.224 ± 0.18
4.386ThrIle: 4.386 ± 0.349
4.243ThrLys: 4.243 ± 0.314
5.344ThrLeu: 5.344 ± 0.351
1.265ThrMet: 1.265 ± 0.153
3.447ThrAsn: 3.447 ± 0.369
2.693ThrPro: 2.693 ± 0.278
2.366ThrGln: 2.366 ± 0.248
2.244ThrArg: 2.244 ± 0.206
4.243ThrSer: 4.243 ± 0.342
4.324ThrThr: 4.324 ± 0.373
5.283ThrVal: 5.283 ± 0.441
0.673ThrTrp: 0.673 ± 0.122
3.223ThrTyr: 3.223 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
4.508ValAla: 4.508 ± 0.292
0.388ValCys: 0.388 ± 0.092
4.365ValAsp: 4.365 ± 0.296
4.651ValGlu: 4.651 ± 0.303
2.835ValPhe: 2.835 ± 0.286
4.182ValGly: 4.182 ± 0.322
1.55ValHis: 1.55 ± 0.153
4.549ValIle: 4.549 ± 0.284
5.528ValLys: 5.528 ± 0.361
5.263ValLeu: 5.263 ± 0.31
1.775ValMet: 1.775 ± 0.168
4.039ValAsn: 4.039 ± 0.3
2.754ValPro: 2.754 ± 0.221
2.407ValGln: 2.407 ± 0.215
3.039ValArg: 3.039 ± 0.245
4.161ValSer: 4.161 ± 0.3
4.732ValThr: 4.732 ± 0.385
4.488ValVal: 4.488 ± 0.364
0.612ValTrp: 0.612 ± 0.111
3.366ValTyr: 3.366 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
0.653TrpAla: 0.653 ± 0.135
0.102TrpCys: 0.102 ± 0.043
0.816TrpAsp: 0.816 ± 0.139
0.816TrpGlu: 0.816 ± 0.123
0.428TrpPhe: 0.428 ± 0.085
0.51TrpGly: 0.51 ± 0.094
0.143TrpHis: 0.143 ± 0.061
0.551TrpIle: 0.551 ± 0.086
0.755TrpLys: 0.755 ± 0.103
0.796TrpLeu: 0.796 ± 0.129
0.326TrpMet: 0.326 ± 0.085
0.551TrpAsn: 0.551 ± 0.104
0.082TrpPro: 0.082 ± 0.038
0.408TrpGln: 0.408 ± 0.082
0.245TrpArg: 0.245 ± 0.063
0.734TrpSer: 0.734 ± 0.112
0.612TrpThr: 0.612 ± 0.114
0.918TrpVal: 0.918 ± 0.142
0.163TrpTrp: 0.163 ± 0.056
0.469TrpTyr: 0.469 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.815TyrAla: 2.815 ± 0.216
0.469TyrCys: 0.469 ± 0.089
2.509TyrAsp: 2.509 ± 0.262
2.937TyrGlu: 2.937 ± 0.257
1.611TyrPhe: 1.611 ± 0.201
2.998TyrGly: 2.998 ± 0.22
0.755TyrHis: 0.755 ± 0.131
2.815TyrIle: 2.815 ± 0.286
3.019TyrLys: 3.019 ± 0.272
3.937TyrLeu: 3.937 ± 0.299
0.999TyrMet: 0.999 ± 0.153
2.876TyrAsn: 2.876 ± 0.257
1.387TyrPro: 1.387 ± 0.148
1.489TyrGln: 1.489 ± 0.19
2.121TyrArg: 2.121 ± 0.216
2.57TyrSer: 2.57 ± 0.278
3.202TyrThr: 3.202 ± 0.265
3.182TyrVal: 3.182 ± 0.234
0.388TyrTrp: 0.388 ± 0.097
1.917TyrTyr: 1.917 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 231 proteins (49026 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski