Helicobacter sp. TUL
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1662 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A2GPK0|A0A2A2GPK0_9HELI Uncharacterized protein (Fragment) OS=Helicobacter sp. TUL OX=1848928 GN=B9T66_08630 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.64 KK3 pKa = 9.8 MIICIICVFGIAYY16 pKa = 9.84 AEE18 pKa = 4.45 PEE20 pKa = 4.23 NSTDD24 pKa = 3.22 ATIKK28 pKa = 10.58 QEE30 pKa = 4.1 SSPSNNVVQNTDD42 pKa = 4.07 PINTDD47 pKa = 3.27 SQNTASMPYY56 pKa = 10.69 DD57 pKa = 4.19 DD58 pKa = 5.28 FTAMMQNANASLNSAMAFAKK78 pKa = 10.2 MGDD81 pKa = 3.82 YY82 pKa = 11.04
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.82
IPC2_protein 4.101
IPC_protein 3.961
Toseland 3.757
ProMoST 4.151
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.795
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.101
DTASelect 4.317
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 1.952
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.977
Protein with the highest isoelectric point:
>tr|A0A2A2GSJ0|A0A2A2GSJ0_9HELI Uncharacterized protein OS=Helicobacter sp. TUL OX=1848928 GN=B9T66_04400 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.09 QPHH8 pKa = 5.34 NTPKK12 pKa = 10.47 KK13 pKa = 7.58 RR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.63 TKK25 pKa = 9.91 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.41 GRR39 pKa = 11.84 KK40 pKa = 8.68 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1662
0
1662
514895
37
2889
309.8
34.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.207 ± 0.048
1.362 ± 0.028
5.205 ± 0.039
6.013 ± 0.061
5.103 ± 0.056
6.162 ± 0.06
2.335 ± 0.032
8.216 ± 0.06
6.935 ± 0.063
10.298 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.025
4.865 ± 0.05
3.338 ± 0.029
4.257 ± 0.045
3.764 ± 0.041
6.821 ± 0.042
5.189 ± 0.05
5.844 ± 0.054
0.78 ± 0.019
3.969 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here