Gordonia phage Huffy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vendettavirus; unclassified Vendettavirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9WS61|A0A1U9WS61_9CAUD Uncharacterized protein OS=Gordonia phage Huffy OX=1958909 GN=80 PE=4 SV=1
MM1 pKa = 7.28TGEE4 pKa = 4.2DD5 pKa = 3.64LGMPEE10 pKa = 4.47YY11 pKa = 10.75ASSGYY16 pKa = 9.28WDD18 pKa = 4.64PEE20 pKa = 4.2CSAHH24 pKa = 7.53GDD26 pKa = 3.58GEE28 pKa = 4.03QWQRR32 pKa = 11.84GKK34 pKa = 11.07ADD36 pKa = 3.22GG37 pKa = 3.94

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9WS58|A0A1U9WS58_9CAUD Uncharacterized protein OS=Gordonia phage Huffy OX=1958909 GN=82 PE=4 SV=1
MM1 pKa = 6.42TAKK4 pKa = 10.53GAGRR8 pKa = 11.84STRR11 pKa = 11.84RR12 pKa = 11.84WRR14 pKa = 11.84RR15 pKa = 11.84LKK17 pKa = 10.92AEE19 pKa = 4.16FRR21 pKa = 11.84RR22 pKa = 11.84LCADD26 pKa = 3.38RR27 pKa = 11.84RR28 pKa = 11.84APCWICGQPIDD39 pKa = 4.08YY40 pKa = 10.13RR41 pKa = 11.84LEE43 pKa = 4.45HH44 pKa = 6.38PHH46 pKa = 6.61PEE48 pKa = 3.64AWEE51 pKa = 4.18PDD53 pKa = 3.45HH54 pKa = 5.81YY55 pKa = 11.25HH56 pKa = 6.6PVAKK60 pKa = 10.35RR61 pKa = 11.84PDD63 pKa = 3.74LAEE66 pKa = 4.67DD67 pKa = 3.7PANLRR72 pKa = 11.84PSHH75 pKa = 6.16SSCNRR80 pKa = 11.84SRR82 pKa = 11.84GDD84 pKa = 3.41RR85 pKa = 11.84EE86 pKa = 4.17VVLGLGRR93 pKa = 11.84QSRR96 pKa = 11.84QWW98 pKa = 3.01

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14107

37

1152

172.0

18.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.611 ± 0.502

0.801 ± 0.124

6.926 ± 0.241

6.6 ± 0.303

2.935 ± 0.168

9.527 ± 0.531

1.935 ± 0.169

3.36 ± 0.165

3.53 ± 0.251

7.493 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.123

2.757 ± 0.15

4.764 ± 0.262

3.027 ± 0.176

7.946 ± 0.405

5.196 ± 0.254

5.607 ± 0.238

8.159 ± 0.294

2.027 ± 0.122

2.502 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski