Xanthomonas phage RiverRider
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1JUQ7|A0A2P1JUQ7_9CAUD Uncharacterized protein OS=Xanthomonas phage RiverRider OX=2108116 GN=RIVERRIDER_4 PE=4 SV=1
MM1 pKa = 7.55 NYY3 pKa = 9.7 IGDD6 pKa = 4.49 NIWHH10 pKa = 6.53 NEE12 pKa = 3.83 VATDD16 pKa = 4.35 LYY18 pKa = 10.4 PVGYY22 pKa = 10.03 YY23 pKa = 10.52 FSDD26 pKa = 3.33 EE27 pKa = 4.38 TNDD30 pKa = 4.1 LNGPYY35 pKa = 10.74 DD36 pKa = 4.12 NDD38 pKa = 3.45 TAAAIAACSHH48 pKa = 5.93 AVSLGG53 pKa = 3.25
Molecular weight: 5.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.908
IPC2_protein 3.681
IPC_protein 3.541
Toseland 3.363
ProMoST 3.795
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.287
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.694
Patrickios 0.006
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.668
Protein with the highest isoelectric point:
>tr|A0A2P1JUT4|A0A2P1JUT4_9CAUD Uncharacterized protein OS=Xanthomonas phage RiverRider OX=2108116 GN=RIVERRIDER_37 PE=4 SV=1
MM1 pKa = 7.93 SIRR4 pKa = 11.84 TRR6 pKa = 11.84 IAGIASAVGATKK18 pKa = 10.5 LAAKK22 pKa = 10.25 LSEE25 pKa = 4.09 EE26 pKa = 4.2 TGQKK30 pKa = 9.5 IVATPEE36 pKa = 3.93 PEE38 pKa = 5.2 GIPSQPEE45 pKa = 4.18 VKK47 pKa = 10.13 RR48 pKa = 11.84 LPRR51 pKa = 11.84 TRR53 pKa = 11.84 EE54 pKa = 3.05 TWYY57 pKa = 10.28 QRR59 pKa = 11.84 WLTEE63 pKa = 3.68 RR64 pKa = 11.84 PQRR67 pKa = 11.84 LRR69 pKa = 11.84 AYY71 pKa = 10.28 ASYY74 pKa = 10.8 KK75 pKa = 9.63 GGKK78 pKa = 6.74 HH79 pKa = 5.03 HH80 pKa = 7.21 RR81 pKa = 11.84 GVPAHH86 pKa = 6.74 IQQQYY91 pKa = 7.95 QFEE94 pKa = 4.73 AEE96 pKa = 4.5 VKK98 pKa = 10.46 RR99 pKa = 11.84 QGKK102 pKa = 7.92 DD103 pKa = 2.6 AKK105 pKa = 10.2 RR106 pKa = 11.84 ARR108 pKa = 11.84 DD109 pKa = 3.6 HH110 pKa = 7.59 SLATYY115 pKa = 10.25 NNPCLKK121 pKa = 10.67 GG122 pKa = 3.41
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.648
IPC_protein 10.087
Toseland 10.482
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 10.994
Grimsley 10.672
Solomon 10.672
Lehninger 10.643
Nozaki 10.452
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.526
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 9.004
IPC2.peptide.svr19 8.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
23209
38
3442
260.8
28.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.358 ± 0.505
0.724 ± 0.136
6.092 ± 0.145
6.118 ± 0.228
3.494 ± 0.153
6.842 ± 0.301
1.784 ± 0.149
5.726 ± 0.262
5.494 ± 0.268
8.355 ± 0.213
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.766 ± 0.138
5.218 ± 0.146
4.149 ± 0.171
4.843 ± 0.259
5.162 ± 0.174
6.183 ± 0.15
6.411 ± 0.221
6.678 ± 0.237
1.314 ± 0.108
3.288 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here