Geovibrio thiophilus

Taxonomy: cellular organisms; Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Deferribacteraceae; Geovibrio

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410JUL7|A0A410JUL7_9BACT 3-isopropylmalate dehydratase OS=Geovibrio thiophilus OX=139438 GN=leuD PE=3 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84CFMKK6 pKa = 9.47KK7 pKa = 8.51TILIIFVIFAFFLYY21 pKa = 10.67GCGADD26 pKa = 4.55DD27 pKa = 5.49GSDD30 pKa = 3.65GADD33 pKa = 3.5GADD36 pKa = 3.87GLTGTQGEE44 pKa = 4.37QGPAGPVGPQGEE56 pKa = 4.27QGPAGPQGEE65 pKa = 4.53PGPAGAAGAVGPAGSDD81 pKa = 2.95GATYY85 pKa = 11.21VLTSPAYY92 pKa = 9.43SADD95 pKa = 3.14VSVAVSANMVFNSVTYY111 pKa = 10.17ASGNISYY118 pKa = 10.86NSVTGIVTFNEE129 pKa = 3.61MGRR132 pKa = 11.84YY133 pKa = 7.33IVNWNVSTDD142 pKa = 3.61GLIGAVNTATADD154 pKa = 3.51LSFALSSSQGDD165 pKa = 4.33FIASSSLLSTGQISGFGIIDD185 pKa = 3.56IAAAPVTMSLINDD198 pKa = 3.85SSNDD202 pKa = 3.4VQLADD207 pKa = 4.11VMPKK211 pKa = 10.48ANLMINRR218 pKa = 11.84SDD220 pKa = 3.58GGVALQISRR229 pKa = 11.84NN230 pKa = 3.69

Molecular weight: 23.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R5UUE6|A0A3R5UUE6_9BACT DNA-directed RNA polymerase subunit beta OS=Geovibrio thiophilus OX=139438 GN=rpoB PE=3 SV=1
MM1 pKa = 7.4LTMKK5 pKa = 10.44KK6 pKa = 10.07PSNLKK11 pKa = 9.92RR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84KK15 pKa = 8.8HH16 pKa = 4.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.72TKK25 pKa = 10.45NGRR28 pKa = 11.84LVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.39GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2687

0

2687

900581

25

2958

335.2

37.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.253 ± 0.057

1.168 ± 0.021

5.583 ± 0.043

7.24 ± 0.054

4.824 ± 0.035

7.442 ± 0.044

1.729 ± 0.019

7.134 ± 0.039

6.769 ± 0.052

9.07 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.837 ± 0.021

4.059 ± 0.027

3.481 ± 0.03

2.369 ± 0.022

4.806 ± 0.041

6.515 ± 0.041

5.388 ± 0.049

7.04 ± 0.037

0.77 ± 0.015

3.522 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski