Amino acid dipepetide frequency for Thermus phage TSP4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.426AlaAla: 7.426 ± 0.856
0.264AlaCys: 0.264 ± 0.099
4.184AlaAsp: 4.184 ± 0.361
7.049AlaGlu: 7.049 ± 1.28
4.071AlaPhe: 4.071 ± 0.333
6.106AlaGly: 6.106 ± 0.691
1.621AlaHis: 1.621 ± 0.336
3.129AlaIle: 3.129 ± 0.284
5.842AlaLys: 5.842 ± 0.795
11.685AlaLeu: 11.685 ± 1.159
2.035AlaMet: 2.035 ± 0.295
2.45AlaAsn: 2.45 ± 0.266
4.071AlaPro: 4.071 ± 0.337
3.882AlaGln: 3.882 ± 0.53
5.541AlaArg: 5.541 ± 0.654
5.918AlaSer: 5.918 ± 0.499
3.053AlaThr: 3.053 ± 0.32
6.257AlaVal: 6.257 ± 0.428
1.282AlaTrp: 1.282 ± 0.206
3.882AlaTyr: 3.882 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
0.302CysAla: 0.302 ± 0.102
0.0CysCys: 0.0 ± 0.0
0.038CysAsp: 0.038 ± 0.037
0.264CysGlu: 0.264 ± 0.098
0.188CysPhe: 0.188 ± 0.1
0.452CysGly: 0.452 ± 0.164
0.113CysHis: 0.113 ± 0.055
0.151CysIle: 0.151 ± 0.078
0.075CysLys: 0.075 ± 0.044
0.49CysLeu: 0.49 ± 0.145
0.188CysMet: 0.188 ± 0.086
0.113CysAsn: 0.113 ± 0.066
0.452CysPro: 0.452 ± 0.163
0.113CysGln: 0.113 ± 0.066
0.188CysArg: 0.188 ± 0.082
0.377CysSer: 0.377 ± 0.154
0.339CysThr: 0.339 ± 0.117
0.302CysVal: 0.302 ± 0.118
0.113CysTrp: 0.113 ± 0.073
0.151CysTyr: 0.151 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
4.335AspAla: 4.335 ± 0.424
0.151AspCys: 0.151 ± 0.062
2.186AspAsp: 2.186 ± 0.321
3.204AspGlu: 3.204 ± 0.29
2.262AspPhe: 2.262 ± 0.31
3.845AspGly: 3.845 ± 0.582
0.905AspHis: 0.905 ± 0.231
2.563AspIle: 2.563 ± 0.353
2.111AspLys: 2.111 ± 0.263
7.199AspLeu: 7.199 ± 0.438
0.867AspMet: 0.867 ± 0.18
1.432AspAsn: 1.432 ± 0.257
3.392AspPro: 3.392 ± 0.329
1.772AspGln: 1.772 ± 0.262
3.091AspArg: 3.091 ± 0.341
2.601AspSer: 2.601 ± 0.313
2.601AspThr: 2.601 ± 0.41
2.375AspVal: 2.375 ± 0.294
1.282AspTrp: 1.282 ± 0.21
2.299AspTyr: 2.299 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
7.991GluAla: 7.991 ± 1.169
0.151GluCys: 0.151 ± 0.076
3.317GluAsp: 3.317 ± 0.364
7.35GluGlu: 7.35 ± 0.869
2.752GluPhe: 2.752 ± 0.264
5.051GluGly: 5.051 ± 0.401
1.319GluHis: 1.319 ± 0.251
2.262GluIle: 2.262 ± 0.236
2.752GluLys: 2.752 ± 0.392
9.235GluLeu: 9.235 ± 0.91
1.47GluMet: 1.47 ± 0.194
1.47GluAsn: 1.47 ± 0.229
3.166GluPro: 3.166 ± 0.424
2.488GluGln: 2.488 ± 0.317
5.202GluArg: 5.202 ± 0.824
2.714GluSer: 2.714 ± 0.264
2.375GluThr: 2.375 ± 0.277
7.011GluVal: 7.011 ± 0.392
1.018GluTrp: 1.018 ± 0.181
2.639GluTyr: 2.639 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
3.053PheAla: 3.053 ± 0.29
0.075PheCys: 0.075 ± 0.05
1.621PheAsp: 1.621 ± 0.206
1.432PheGlu: 1.432 ± 0.208
1.47PhePhe: 1.47 ± 0.215
3.053PheGly: 3.053 ± 0.312
1.093PheHis: 1.093 ± 0.243
1.545PheIle: 1.545 ± 0.204
1.809PheLys: 1.809 ± 0.235
4.523PheLeu: 4.523 ± 0.431
0.905PheMet: 0.905 ± 0.16
1.395PheAsn: 1.395 ± 0.212
1.96PhePro: 1.96 ± 0.45
1.282PheGln: 1.282 ± 0.188
2.412PheArg: 2.412 ± 0.284
2.978PheSer: 2.978 ± 0.365
2.525PheThr: 2.525 ± 0.346
2.865PheVal: 2.865 ± 0.329
0.377PheTrp: 0.377 ± 0.148
1.885PheTyr: 1.885 ± 0.261
0.0PheXaa: 0.0 ± 0.0
Gly
4.712GlyAla: 4.712 ± 0.48
0.038GlyCys: 0.038 ± 0.034
2.752GlyAsp: 2.752 ± 0.302
4.9GlyGlu: 4.9 ± 0.384
3.204GlyPhe: 3.204 ± 0.36
5.051GlyGly: 5.051 ± 0.608
1.545GlyHis: 1.545 ± 0.296
3.129GlyIle: 3.129 ± 0.312
3.166GlyLys: 3.166 ± 0.415
8.519GlyLeu: 8.519 ± 1.014
2.375GlyMet: 2.375 ± 0.359
2.639GlyAsn: 2.639 ± 0.345
2.563GlyPro: 2.563 ± 0.301
3.015GlyGln: 3.015 ± 0.365
4.523GlyArg: 4.523 ± 0.371
4.184GlySer: 4.184 ± 0.407
3.92GlyThr: 3.92 ± 0.341
6.408GlyVal: 6.408 ± 0.446
1.395GlyTrp: 1.395 ± 0.331
3.166GlyTyr: 3.166 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.357HisAla: 1.357 ± 0.301
0.075HisCys: 0.075 ± 0.047
0.942HisAsp: 0.942 ± 0.221
0.754HisGlu: 0.754 ± 0.17
0.415HisPhe: 0.415 ± 0.115
1.508HisGly: 1.508 ± 0.288
0.302HisHis: 0.302 ± 0.121
1.055HisIle: 1.055 ± 0.243
0.603HisLys: 0.603 ± 0.116
2.525HisLeu: 2.525 ± 0.402
0.151HisMet: 0.151 ± 0.069
0.603HisAsn: 0.603 ± 0.178
1.621HisPro: 1.621 ± 0.313
0.754HisGln: 0.754 ± 0.169
1.282HisArg: 1.282 ± 0.255
1.093HisSer: 1.093 ± 0.302
1.093HisThr: 1.093 ± 0.211
1.206HisVal: 1.206 ± 0.278
0.302HisTrp: 0.302 ± 0.134
0.49HisTyr: 0.49 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
3.807IleAla: 3.807 ± 0.351
0.188IleCys: 0.188 ± 0.078
1.621IleAsp: 1.621 ± 0.255
2.262IleGlu: 2.262 ± 0.288
0.98IlePhe: 0.98 ± 0.192
2.865IleGly: 2.865 ± 0.306
0.678IleHis: 0.678 ± 0.197
1.545IleIle: 1.545 ± 0.254
1.885IleLys: 1.885 ± 0.249
4.146IleLeu: 4.146 ± 0.349
0.792IleMet: 0.792 ± 0.198
1.357IleAsn: 1.357 ± 0.181
2.714IlePro: 2.714 ± 0.312
1.922IleGln: 1.922 ± 0.235
2.714IleArg: 2.714 ± 0.264
1.772IleSer: 1.772 ± 0.263
2.149IleThr: 2.149 ± 0.347
2.488IleVal: 2.488 ± 0.369
0.49IleTrp: 0.49 ± 0.147
1.508IleTyr: 1.508 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
5.767LysAla: 5.767 ± 0.684
0.0LysCys: 0.0 ± 0.0
2.752LysAsp: 2.752 ± 0.249
4.674LysGlu: 4.674 ± 0.648
1.319LysPhe: 1.319 ± 0.267
3.958LysGly: 3.958 ± 0.389
0.867LysHis: 0.867 ± 0.196
1.093LysIle: 1.093 ± 0.177
1.96LysLys: 1.96 ± 0.279
5.842LysLeu: 5.842 ± 0.462
0.452LysMet: 0.452 ± 0.128
1.244LysAsn: 1.244 ± 0.207
2.789LysPro: 2.789 ± 0.368
1.922LysGln: 1.922 ± 0.522
3.732LysArg: 3.732 ± 0.418
2.035LysSer: 2.035 ± 0.222
2.865LysThr: 2.865 ± 0.307
4.146LysVal: 4.146 ± 0.295
0.377LysTrp: 0.377 ± 0.122
1.885LysTyr: 1.885 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
11.119LeuAla: 11.119 ± 1.046
0.565LeuCys: 0.565 ± 0.154
7.199LeuAsp: 7.199 ± 0.439
9.235LeuGlu: 9.235 ± 0.749
3.656LeuPhe: 3.656 ± 0.293
7.463LeuGly: 7.463 ± 0.817
2.488LeuHis: 2.488 ± 0.399
3.732LeuIle: 3.732 ± 0.348
7.199LeuLys: 7.199 ± 0.821
10.667LeuLeu: 10.667 ± 0.602
2.111LeuMet: 2.111 ± 0.25
3.845LeuAsn: 3.845 ± 0.397
5.993LeuPro: 5.993 ± 0.495
5.88LeuGln: 5.88 ± 0.445
6.521LeuArg: 6.521 ± 0.412
7.991LeuSer: 7.991 ± 0.84
6.031LeuThr: 6.031 ± 0.498
8.707LeuVal: 8.707 ± 0.487
1.357LeuTrp: 1.357 ± 0.282
4.599LeuTyr: 4.599 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.149MetAla: 2.149 ± 0.374
0.188MetCys: 0.188 ± 0.101
1.357MetAsp: 1.357 ± 0.195
1.772MetGlu: 1.772 ± 0.276
0.49MetPhe: 0.49 ± 0.126
1.395MetGly: 1.395 ± 0.257
0.302MetHis: 0.302 ± 0.119
0.829MetIle: 0.829 ± 0.248
0.565MetLys: 0.565 ± 0.173
2.035MetLeu: 2.035 ± 0.264
0.377MetMet: 0.377 ± 0.101
0.905MetAsn: 0.905 ± 0.182
1.131MetPro: 1.131 ± 0.232
0.678MetGln: 0.678 ± 0.266
1.658MetArg: 1.658 ± 0.213
1.244MetSer: 1.244 ± 0.212
1.395MetThr: 1.395 ± 0.217
1.432MetVal: 1.432 ± 0.21
0.151MetTrp: 0.151 ± 0.066
0.452MetTyr: 0.452 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
2.639AsnAla: 2.639 ± 0.32
0.188AsnCys: 0.188 ± 0.081
1.357AsnAsp: 1.357 ± 0.268
1.508AsnGlu: 1.508 ± 0.201
1.131AsnPhe: 1.131 ± 0.221
3.392AsnGly: 3.392 ± 0.508
0.603AsnHis: 0.603 ± 0.177
1.168AsnIle: 1.168 ± 0.196
1.206AsnLys: 1.206 ± 0.195
4.297AsnLeu: 4.297 ± 0.473
0.528AsnMet: 0.528 ± 0.163
1.319AsnAsn: 1.319 ± 0.282
2.262AsnPro: 2.262 ± 0.305
1.658AsnGln: 1.658 ± 0.264
2.299AsnArg: 2.299 ± 0.26
1.847AsnSer: 1.847 ± 0.195
1.998AsnThr: 1.998 ± 0.302
1.47AsnVal: 1.47 ± 0.277
0.641AsnTrp: 0.641 ± 0.139
0.867AsnTyr: 0.867 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
3.845ProAla: 3.845 ± 0.311
0.377ProCys: 0.377 ± 0.142
3.468ProAsp: 3.468 ± 0.485
4.109ProGlu: 4.109 ± 0.351
2.224ProPhe: 2.224 ± 0.302
3.958ProGly: 3.958 ± 0.493
0.942ProHis: 0.942 ± 0.229
2.45ProIle: 2.45 ± 0.318
3.355ProLys: 3.355 ± 0.385
5.239ProLeu: 5.239 ± 0.593
0.754ProMet: 0.754 ± 0.143
1.922ProAsn: 1.922 ± 0.265
2.601ProPro: 2.601 ± 0.304
1.47ProGln: 1.47 ± 0.247
2.337ProArg: 2.337 ± 0.366
3.392ProSer: 3.392 ± 0.407
2.789ProThr: 2.789 ± 0.314
3.581ProVal: 3.581 ± 0.483
0.716ProTrp: 0.716 ± 0.182
2.488ProTyr: 2.488 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
5.164GlnAla: 5.164 ± 0.692
0.113GlnCys: 0.113 ± 0.057
1.885GlnAsp: 1.885 ± 0.279
3.581GlnGlu: 3.581 ± 0.352
1.244GlnPhe: 1.244 ± 0.218
2.94GlnGly: 2.94 ± 0.301
0.377GlnHis: 0.377 ± 0.12
1.432GlnIle: 1.432 ± 0.233
2.262GlnLys: 2.262 ± 0.46
5.88GlnLeu: 5.88 ± 0.94
0.754GlnMet: 0.754 ± 0.166
1.432GlnAsn: 1.432 ± 0.267
1.508GlnPro: 1.508 ± 0.215
1.696GlnGln: 1.696 ± 0.37
2.337GlnArg: 2.337 ± 0.266
1.772GlnSer: 1.772 ± 0.228
1.357GlnThr: 1.357 ± 0.262
4.033GlnVal: 4.033 ± 0.414
0.528GlnTrp: 0.528 ± 0.145
1.282GlnTyr: 1.282 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
5.164ArgAla: 5.164 ± 0.521
0.377ArgCys: 0.377 ± 0.128
2.601ArgAsp: 2.601 ± 0.323
4.749ArgGlu: 4.749 ± 0.613
2.827ArgPhe: 2.827 ± 0.287
2.789ArgGly: 2.789 ± 0.289
0.867ArgHis: 0.867 ± 0.169
3.091ArgIle: 3.091 ± 0.321
3.392ArgLys: 3.392 ± 0.423
7.689ArgLeu: 7.689 ± 0.608
1.922ArgMet: 1.922 ± 0.218
1.998ArgAsn: 1.998 ± 0.274
2.488ArgPro: 2.488 ± 0.417
2.94ArgGln: 2.94 ± 0.605
4.523ArgArg: 4.523 ± 0.397
3.581ArgSer: 3.581 ± 0.39
3.581ArgThr: 3.581 ± 0.383
5.428ArgVal: 5.428 ± 0.524
1.168ArgTrp: 1.168 ± 0.261
3.619ArgTyr: 3.619 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
5.013SerAla: 5.013 ± 0.496
0.603SerCys: 0.603 ± 0.144
3.129SerAsp: 3.129 ± 0.495
3.053SerGlu: 3.053 ± 0.284
2.149SerPhe: 2.149 ± 0.27
4.825SerGly: 4.825 ± 0.351
0.716SerHis: 0.716 ± 0.172
2.111SerIle: 2.111 ± 0.289
2.714SerLys: 2.714 ± 0.281
6.332SerLeu: 6.332 ± 0.464
1.583SerMet: 1.583 ± 0.212
1.734SerAsn: 1.734 ± 0.233
2.978SerPro: 2.978 ± 0.284
2.111SerGln: 2.111 ± 0.353
4.297SerArg: 4.297 ± 0.363
3.882SerSer: 3.882 ± 0.601
3.053SerThr: 3.053 ± 0.293
5.202SerVal: 5.202 ± 0.495
0.829SerTrp: 0.829 ± 0.182
1.922SerTyr: 1.922 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
4.523ThrAla: 4.523 ± 0.332
0.302ThrCys: 0.302 ± 0.12
2.337ThrAsp: 2.337 ± 0.233
3.015ThrGlu: 3.015 ± 0.246
2.299ThrPhe: 2.299 ± 0.25
3.619ThrGly: 3.619 ± 0.36
0.98ThrHis: 0.98 ± 0.183
2.262ThrIle: 2.262 ± 0.28
2.563ThrLys: 2.563 ± 0.278
5.767ThrLeu: 5.767 ± 0.422
0.678ThrMet: 0.678 ± 0.21
1.395ThrAsn: 1.395 ± 0.208
3.204ThrPro: 3.204 ± 0.326
1.658ThrGln: 1.658 ± 0.232
2.412ThrArg: 2.412 ± 0.323
3.053ThrSer: 3.053 ± 0.332
1.847ThrThr: 1.847 ± 0.372
4.599ThrVal: 4.599 ± 0.601
0.716ThrTrp: 0.716 ± 0.253
1.847ThrTyr: 1.847 ± 0.309
0.0ThrXaa: 0.0 ± 0.0
Val
5.729ValAla: 5.729 ± 0.449
0.377ValCys: 0.377 ± 0.137
4.787ValAsp: 4.787 ± 0.407
5.202ValGlu: 5.202 ± 0.373
3.619ValPhe: 3.619 ± 0.475
4.674ValGly: 4.674 ± 0.389
1.508ValHis: 1.508 ± 0.316
2.525ValIle: 2.525 ± 0.389
3.845ValLys: 3.845 ± 0.352
8.669ValLeu: 8.669 ± 0.624
1.395ValMet: 1.395 ± 0.205
2.639ValAsn: 2.639 ± 0.272
4.41ValPro: 4.41 ± 0.671
3.958ValGln: 3.958 ± 0.477
5.202ValArg: 5.202 ± 0.347
4.749ValSer: 4.749 ± 0.408
2.752ValThr: 2.752 ± 0.348
5.993ValVal: 5.993 ± 0.71
1.432ValTrp: 1.432 ± 0.311
3.656ValTyr: 3.656 ± 0.397
0.0ValXaa: 0.0 ± 0.0
Trp
1.206TrpAla: 1.206 ± 0.202
0.075TrpCys: 0.075 ± 0.058
0.905TrpAsp: 0.905 ± 0.203
0.942TrpGlu: 0.942 ± 0.243
0.528TrpPhe: 0.528 ± 0.142
0.754TrpGly: 0.754 ± 0.158
0.339TrpHis: 0.339 ± 0.12
0.754TrpIle: 0.754 ± 0.164
0.603TrpLys: 0.603 ± 0.164
1.734TrpLeu: 1.734 ± 0.25
0.226TrpMet: 0.226 ± 0.086
0.754TrpAsn: 0.754 ± 0.203
0.716TrpPro: 0.716 ± 0.198
0.603TrpGln: 0.603 ± 0.133
1.131TrpArg: 1.131 ± 0.239
0.754TrpSer: 0.754 ± 0.181
0.867TrpThr: 0.867 ± 0.236
1.131TrpVal: 1.131 ± 0.284
0.302TrpTrp: 0.302 ± 0.118
0.867TrpTyr: 0.867 ± 0.26
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.448TyrAla: 4.448 ± 0.439
0.302TyrCys: 0.302 ± 0.112
2.035TyrAsp: 2.035 ± 0.248
2.676TyrGlu: 2.676 ± 0.324
1.432TyrPhe: 1.432 ± 0.243
3.505TyrGly: 3.505 ± 0.372
0.565TyrHis: 0.565 ± 0.184
1.055TyrIle: 1.055 ± 0.223
1.772TyrLys: 1.772 ± 0.248
3.882TyrLeu: 3.882 ± 0.332
0.905TyrMet: 0.905 ± 0.226
1.734TyrAsn: 1.734 ± 0.233
1.998TyrPro: 1.998 ± 0.371
1.809TyrGln: 1.809 ± 0.216
3.392TyrArg: 3.392 ± 0.321
2.299TyrSer: 2.299 ± 0.261
2.488TyrThr: 2.488 ± 0.412
2.488TyrVal: 2.488 ± 0.478
0.716TyrTrp: 0.716 ± 0.115
1.734TyrTyr: 1.734 ± 0.358
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (26531 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski