Streptomyces sp. 8K308

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4PL97|A0A4R4PL97_9ACTN Glycoside hydrolase OS=Streptomyces sp. 8K308 OX=2530388 GN=E1265_14805 PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84GARR5 pKa = 11.84SAKK8 pKa = 8.19WVVSAIVVAMAATACGSDD26 pKa = 3.77SDD28 pKa = 5.22DD29 pKa = 4.37NGDD32 pKa = 3.69GGASGEE38 pKa = 4.3GNSAGIVRR46 pKa = 11.84VDD48 pKa = 3.27GGEE51 pKa = 4.01PQNPLIPTNTTEE63 pKa = 3.86QYY65 pKa = 10.31GALVIQNVWSKK76 pKa = 11.06LVDD79 pKa = 4.0FDD81 pKa = 5.48DD82 pKa = 3.76QGQVFTVAAEE92 pKa = 4.18SVEE95 pKa = 4.22PNEE98 pKa = 4.83DD99 pKa = 3.17ASVWTVTLKK108 pKa = 10.99DD109 pKa = 2.91GWSFHH114 pKa = 6.3NGEE117 pKa = 4.3AVTAQSYY124 pKa = 9.45VDD126 pKa = 3.38AWNWAANVNNNQQNSFWFSDD146 pKa = 2.9IAGYY150 pKa = 10.92EE151 pKa = 4.04DD152 pKa = 3.51VHH154 pKa = 8.1PEE156 pKa = 3.91EE157 pKa = 5.41GEE159 pKa = 4.08PTADD163 pKa = 3.03TMSGLEE169 pKa = 4.11VVDD172 pKa = 5.45DD173 pKa = 3.96LTFTITLNTPQSYY186 pKa = 8.71YY187 pKa = 10.91DD188 pKa = 3.47YY189 pKa = 10.98RR190 pKa = 11.84LGYY193 pKa = 10.28DD194 pKa = 3.83AFAPLPSVFYY204 pKa = 10.39DD205 pKa = 3.51DD206 pKa = 4.88PEE208 pKa = 5.64AFGEE212 pKa = 4.26HH213 pKa = 7.31PIGNGPYY220 pKa = 10.52EE221 pKa = 4.05FTEE224 pKa = 4.23WNHH227 pKa = 5.74NEE229 pKa = 4.39SISLTAWDD237 pKa = 5.32EE238 pKa = 4.24YY239 pKa = 10.97PGDD242 pKa = 4.52DD243 pKa = 3.75KK244 pKa = 11.22PANGGVEE251 pKa = 3.92LVAYY255 pKa = 9.86DD256 pKa = 4.27NLHH259 pKa = 5.96TAYY262 pKa = 10.48QDD264 pKa = 3.88LLSGNLDD271 pKa = 3.36VLRR274 pKa = 11.84EE275 pKa = 3.85VDD277 pKa = 3.84AQDD280 pKa = 3.62LPVYY284 pKa = 8.81EE285 pKa = 4.45TDD287 pKa = 3.66LGDD290 pKa = 3.51RR291 pKa = 11.84AIAQPYY297 pKa = 10.19NGTQSIVPAFYY308 pKa = 10.34SDD310 pKa = 3.41EE311 pKa = 4.6FEE313 pKa = 5.3DD314 pKa = 3.31VDD316 pKa = 3.88PRR318 pKa = 11.84VLQGMSLAIDD328 pKa = 3.68RR329 pKa = 11.84EE330 pKa = 4.73TITGTVLNGSATPADD345 pKa = 3.99SFVAPGVFGYY355 pKa = 10.15QEE357 pKa = 4.54GIGAEE362 pKa = 4.15FTAYY366 pKa = 10.28DD367 pKa = 3.73PEE369 pKa = 4.23RR370 pKa = 11.84ARR372 pKa = 11.84EE373 pKa = 3.96LFEE376 pKa = 6.12AGGGIPGNTVTIQYY390 pKa = 10.11NADD393 pKa = 3.32GPHH396 pKa = 6.35RR397 pKa = 11.84EE398 pKa = 4.05WVEE401 pKa = 3.9AVCGNLRR408 pKa = 11.84DD409 pKa = 4.03NLGVEE414 pKa = 4.73CEE416 pKa = 4.21TDD418 pKa = 3.63VKK420 pKa = 11.04PDD422 pKa = 3.6FATDD426 pKa = 3.79LEE428 pKa = 4.4ARR430 pKa = 11.84DD431 pKa = 3.62ADD433 pKa = 4.19EE434 pKa = 3.85VVGMYY439 pKa = 10.28RR440 pKa = 11.84GGWFQDD446 pKa = 3.39YY447 pKa = 9.82PLNVNFLRR455 pKa = 11.84EE456 pKa = 4.37LYY458 pKa = 9.02RR459 pKa = 11.84TGASTNYY466 pKa = 9.4GRR468 pKa = 11.84YY469 pKa = 9.79SNEE472 pKa = 3.49EE473 pKa = 3.4VDD475 pKa = 4.48ALFAEE480 pKa = 5.19GDD482 pKa = 3.76SSASLDD488 pKa = 3.75DD489 pKa = 3.52TVAAYY494 pKa = 10.14QEE496 pKa = 4.38AEE498 pKa = 3.99EE499 pKa = 5.61LLFQDD504 pKa = 4.56MPAIPLWFQNINGGYY519 pKa = 9.14SEE521 pKa = 5.21NVDD524 pKa = 3.1NVRR527 pKa = 11.84FDD529 pKa = 3.4MAGQPVLTEE538 pKa = 3.89VTVNN542 pKa = 3.26

Molecular weight: 59.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4NTH5|A0A4R4NTH5_9ACTN PAS domain-containing protein (Fragment) OS=Streptomyces sp. 8K308 OX=2530388 GN=E1265_30100 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.43GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6672

0

6672

2115711

18

3386

317.1

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.063 ± 0.054

0.758 ± 0.009

6.001 ± 0.026

6.025 ± 0.031

2.679 ± 0.017

9.483 ± 0.025

2.288 ± 0.013

3.069 ± 0.022

1.293 ± 0.02

10.674 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.631 ± 0.013

1.719 ± 0.017

6.208 ± 0.024

2.576 ± 0.017

8.8 ± 0.035

4.75 ± 0.023

6.008 ± 0.027

8.402 ± 0.027

1.578 ± 0.013

1.997 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski