Amino acid dipepetide frequency for Rhizobium phage RL2RES

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.647AlaAla: 4.647 ± 0.354
0.55AlaCys: 0.55 ± 0.108
4.138AlaAsp: 4.138 ± 0.319
4.24AlaGlu: 4.24 ± 0.304
2.772AlaPhe: 2.772 ± 0.275
4.077AlaGly: 4.077 ± 0.463
0.958AlaHis: 0.958 ± 0.123
4.586AlaIle: 4.586 ± 0.309
4.851AlaLys: 4.851 ± 0.33
5.605AlaLeu: 5.605 ± 0.313
1.733AlaMet: 1.733 ± 0.197
3.037AlaAsn: 3.037 ± 0.282
2.161AlaPro: 2.161 ± 0.255
1.733AlaGln: 1.733 ± 0.202
3.404AlaArg: 3.404 ± 0.233
4.79AlaSer: 4.79 ± 0.344
3.669AlaThr: 3.669 ± 0.323
4.464AlaVal: 4.464 ± 0.306
0.836AlaTrp: 0.836 ± 0.122
2.14AlaTyr: 2.14 ± 0.171
0.0AlaXaa: 0.0 ± 0.0
Cys
0.469CysAla: 0.469 ± 0.102
0.102CysCys: 0.102 ± 0.052
0.571CysAsp: 0.571 ± 0.122
0.591CysGlu: 0.591 ± 0.107
0.489CysPhe: 0.489 ± 0.099
0.489CysGly: 0.489 ± 0.107
0.204CysHis: 0.204 ± 0.066
0.428CysIle: 0.428 ± 0.103
0.693CysLys: 0.693 ± 0.121
0.693CysLeu: 0.693 ± 0.117
0.143CysMet: 0.143 ± 0.062
0.387CysAsn: 0.387 ± 0.08
0.306CysPro: 0.306 ± 0.073
0.347CysGln: 0.347 ± 0.083
0.734CysArg: 0.734 ± 0.108
0.285CysSer: 0.285 ± 0.075
0.347CysThr: 0.347 ± 0.105
0.856CysVal: 0.856 ± 0.145
0.061CysTrp: 0.061 ± 0.036
0.306CysTyr: 0.306 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
4.403AspAla: 4.403 ± 0.34
0.53AspCys: 0.53 ± 0.099
4.892AspAsp: 4.892 ± 0.375
5.3AspGlu: 5.3 ± 0.352
4.036AspPhe: 4.036 ± 0.314
4.708AspGly: 4.708 ± 0.372
1.366AspHis: 1.366 ± 0.153
4.464AspIle: 4.464 ± 0.258
4.464AspLys: 4.464 ± 0.307
5.911AspLeu: 5.911 ± 0.393
1.834AspMet: 1.834 ± 0.207
2.894AspAsn: 2.894 ± 0.238
2.935AspPro: 2.935 ± 0.224
2.161AspGln: 2.161 ± 0.235
3.363AspArg: 3.363 ± 0.24
3.547AspSer: 3.547 ± 0.303
3.343AspThr: 3.343 ± 0.239
4.973AspVal: 4.973 ± 0.39
1.121AspTrp: 1.121 ± 0.174
3.22AspTyr: 3.22 ± 0.287
0.0AspXaa: 0.0 ± 0.0
Glu
4.464GluAla: 4.464 ± 0.363
0.611GluCys: 0.611 ± 0.116
4.178GluAsp: 4.178 ± 0.294
5.728GluGlu: 5.728 ± 0.497
4.015GluPhe: 4.015 ± 0.321
3.424GluGly: 3.424 ± 0.244
1.243GluHis: 1.243 ± 0.152
5.87GluIle: 5.87 ± 0.389
5.218GluLys: 5.218 ± 0.398
6.074GluLeu: 6.074 ± 0.342
2.12GluMet: 2.12 ± 0.22
3.75GluAsn: 3.75 ± 0.261
1.936GluPro: 1.936 ± 0.186
1.957GluGln: 1.957 ± 0.203
3.526GluArg: 3.526 ± 0.315
3.343GluSer: 3.343 ± 0.244
4.077GluThr: 4.077 ± 0.281
4.627GluVal: 4.627 ± 0.332
1.019GluTrp: 1.019 ± 0.13
2.548GluTyr: 2.548 ± 0.235
0.0GluXaa: 0.0 ± 0.0
Phe
3.119PheAla: 3.119 ± 0.275
0.591PheCys: 0.591 ± 0.112
4.607PheAsp: 4.607 ± 0.286
4.403PheGlu: 4.403 ± 0.351
2.385PhePhe: 2.385 ± 0.21
3.282PheGly: 3.282 ± 0.245
0.815PheHis: 0.815 ± 0.115
2.691PheIle: 2.691 ± 0.228
3.363PheLys: 3.363 ± 0.331
3.71PheLeu: 3.71 ± 0.294
1.406PheMet: 1.406 ± 0.192
2.507PheAsn: 2.507 ± 0.256
1.304PhePro: 1.304 ± 0.177
1.284PheGln: 1.284 ± 0.191
2.303PheArg: 2.303 ± 0.278
3.363PheSer: 3.363 ± 0.246
3.465PheThr: 3.465 ± 0.25
3.852PheVal: 3.852 ± 0.324
0.632PheTrp: 0.632 ± 0.119
1.651PheTyr: 1.651 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
3.628GlyAla: 3.628 ± 0.4
0.53GlyCys: 0.53 ± 0.114
4.729GlyAsp: 4.729 ± 0.389
3.73GlyGlu: 3.73 ± 0.291
2.915GlyPhe: 2.915 ± 0.249
4.443GlyGly: 4.443 ± 0.666
1.203GlyHis: 1.203 ± 0.155
3.608GlyIle: 3.608 ± 0.338
3.995GlyLys: 3.995 ± 0.313
4.056GlyLeu: 4.056 ± 0.273
1.733GlyMet: 1.733 ± 0.231
3.445GlyAsn: 3.445 ± 0.305
1.814GlyPro: 1.814 ± 0.182
2.161GlyGln: 2.161 ± 0.198
3.017GlyArg: 3.017 ± 0.221
5.035GlySer: 5.035 ± 0.444
4.525GlyThr: 4.525 ± 0.45
3.913GlyVal: 3.913 ± 0.301
0.856GlyTrp: 0.856 ± 0.123
2.629GlyTyr: 2.629 ± 0.213
0.0GlyXaa: 0.0 ± 0.0
His
1.182HisAla: 1.182 ± 0.157
0.224HisCys: 0.224 ± 0.066
1.08HisAsp: 1.08 ± 0.147
1.06HisGlu: 1.06 ± 0.151
1.121HisPhe: 1.121 ± 0.162
1.162HisGly: 1.162 ± 0.166
0.51HisHis: 0.51 ± 0.104
1.019HisIle: 1.019 ± 0.127
0.938HisLys: 0.938 ± 0.145
1.753HisLeu: 1.753 ± 0.219
0.469HisMet: 0.469 ± 0.117
0.917HisAsn: 0.917 ± 0.123
0.876HisPro: 0.876 ± 0.154
0.754HisGln: 0.754 ± 0.137
0.938HisArg: 0.938 ± 0.133
0.856HisSer: 0.856 ± 0.134
1.08HisThr: 1.08 ± 0.143
1.488HisVal: 1.488 ± 0.187
0.245HisTrp: 0.245 ± 0.066
0.815HisTyr: 0.815 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
4.79IleAla: 4.79 ± 0.294
0.469IleCys: 0.469 ± 0.094
5.687IleAsp: 5.687 ± 0.338
4.871IleGlu: 4.871 ± 0.312
2.956IlePhe: 2.956 ± 0.254
4.729IleGly: 4.729 ± 0.383
1.223IleHis: 1.223 ± 0.145
3.547IleIle: 3.547 ± 0.226
4.097IleLys: 4.097 ± 0.283
4.199IleLeu: 4.199 ± 0.262
1.101IleMet: 1.101 ± 0.137
3.567IleAsn: 3.567 ± 0.327
2.487IlePro: 2.487 ± 0.2
2.201IleGln: 2.201 ± 0.211
3.424IleArg: 3.424 ± 0.23
4.81IleSer: 4.81 ± 0.288
3.873IleThr: 3.873 ± 0.29
5.422IleVal: 5.422 ± 0.341
0.815IleTrp: 0.815 ± 0.143
2.426IleTyr: 2.426 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
4.077LysAla: 4.077 ± 0.294
0.53LysCys: 0.53 ± 0.108
4.24LysAsp: 4.24 ± 0.296
4.77LysGlu: 4.77 ± 0.356
4.077LysPhe: 4.077 ± 0.295
2.792LysGly: 2.792 ± 0.266
1.141LysHis: 1.141 ± 0.163
5.952LysIle: 5.952 ± 0.416
5.544LysLys: 5.544 ± 0.447
5.3LysLeu: 5.3 ± 0.383
2.344LysMet: 2.344 ± 0.265
4.056LysAsn: 4.056 ± 0.284
1.896LysPro: 1.896 ± 0.236
2.426LysGln: 2.426 ± 0.206
3.485LysArg: 3.485 ± 0.303
3.832LysSer: 3.832 ± 0.215
4.79LysThr: 4.79 ± 0.298
4.362LysVal: 4.362 ± 0.331
0.856LysTrp: 0.856 ± 0.142
2.548LysTyr: 2.548 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
5.524LeuAla: 5.524 ± 0.374
0.734LeuCys: 0.734 ± 0.124
5.463LeuAsp: 5.463 ± 0.33
5.463LeuGlu: 5.463 ± 0.342
3.363LeuPhe: 3.363 ± 0.238
4.24LeuGly: 4.24 ± 0.326
1.366LeuHis: 1.366 ± 0.175
4.545LeuIle: 4.545 ± 0.318
5.789LeuLys: 5.789 ± 0.391
5.218LeuLeu: 5.218 ± 0.35
1.834LeuMet: 1.834 ± 0.223
4.382LeuAsn: 4.382 ± 0.272
3.119LeuPro: 3.119 ± 0.284
2.711LeuGln: 2.711 ± 0.207
4.097LeuArg: 4.097 ± 0.319
5.483LeuSer: 5.483 ± 0.356
4.79LeuThr: 4.79 ± 0.378
5.035LeuVal: 5.035 ± 0.366
0.876LeuTrp: 0.876 ± 0.121
2.752LeuTyr: 2.752 ± 0.32
0.0LeuXaa: 0.0 ± 0.0
Met
1.814MetAla: 1.814 ± 0.173
0.163MetCys: 0.163 ± 0.053
0.999MetAsp: 0.999 ± 0.156
1.569MetGlu: 1.569 ± 0.185
1.141MetPhe: 1.141 ± 0.152
0.917MetGly: 0.917 ± 0.147
0.53MetHis: 0.53 ± 0.092
1.549MetIle: 1.549 ± 0.188
2.609MetLys: 2.609 ± 0.222
1.529MetLeu: 1.529 ± 0.206
0.632MetMet: 0.632 ± 0.122
1.427MetAsn: 1.427 ± 0.163
0.958MetPro: 0.958 ± 0.135
0.917MetGln: 0.917 ± 0.155
1.468MetArg: 1.468 ± 0.182
2.364MetSer: 2.364 ± 0.234
2.426MetThr: 2.426 ± 0.222
1.141MetVal: 1.141 ± 0.15
0.224MetTrp: 0.224 ± 0.062
0.693MetTyr: 0.693 ± 0.11
0.0MetXaa: 0.0 ± 0.0
Asn
3.934AsnAla: 3.934 ± 0.314
0.326AsnCys: 0.326 ± 0.09
3.587AsnAsp: 3.587 ± 0.242
4.015AsnGlu: 4.015 ± 0.285
2.711AsnPhe: 2.711 ± 0.208
4.28AsnGly: 4.28 ± 0.313
0.876AsnHis: 0.876 ± 0.124
3.139AsnIle: 3.139 ± 0.261
2.65AsnLys: 2.65 ± 0.263
3.975AsnLeu: 3.975 ± 0.298
1.406AsnMet: 1.406 ± 0.174
2.629AsnAsn: 2.629 ± 0.218
2.426AsnPro: 2.426 ± 0.207
1.366AsnGln: 1.366 ± 0.15
2.691AsnArg: 2.691 ± 0.242
3.608AsnSer: 3.608 ± 0.294
2.527AsnThr: 2.527 ± 0.22
3.485AsnVal: 3.485 ± 0.294
0.611AsnTrp: 0.611 ± 0.119
2.222AsnTyr: 2.222 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
1.834ProAla: 1.834 ± 0.196
0.204ProCys: 0.204 ± 0.058
2.364ProAsp: 2.364 ± 0.262
2.854ProGlu: 2.854 ± 0.242
1.508ProPhe: 1.508 ± 0.208
2.548ProGly: 2.548 ± 0.27
0.693ProHis: 0.693 ± 0.141
2.161ProIle: 2.161 ± 0.192
1.998ProLys: 1.998 ± 0.205
2.691ProLeu: 2.691 ± 0.24
0.632ProMet: 0.632 ± 0.111
1.733ProAsn: 1.733 ± 0.202
0.795ProPro: 0.795 ± 0.118
0.836ProGln: 0.836 ± 0.128
1.529ProArg: 1.529 ± 0.198
2.833ProSer: 2.833 ± 0.255
2.222ProThr: 2.222 ± 0.191
3.078ProVal: 3.078 ± 0.244
0.448ProTrp: 0.448 ± 0.102
1.447ProTyr: 1.447 ± 0.192
0.0ProXaa: 0.0 ± 0.0
Gln
1.834GlnAla: 1.834 ± 0.21
0.285GlnCys: 0.285 ± 0.083
1.753GlnAsp: 1.753 ± 0.201
2.344GlnGlu: 2.344 ± 0.207
1.549GlnPhe: 1.549 ± 0.195
1.427GlnGly: 1.427 ± 0.157
0.408GlnHis: 0.408 ± 0.09
2.466GlnIle: 2.466 ± 0.203
2.527GlnLys: 2.527 ± 0.263
2.609GlnLeu: 2.609 ± 0.223
0.815GlnMet: 0.815 ± 0.148
2.283GlnAsn: 2.283 ± 0.226
0.673GlnPro: 0.673 ± 0.124
1.284GlnGln: 1.284 ± 0.148
1.631GlnArg: 1.631 ± 0.185
1.753GlnSer: 1.753 ± 0.213
2.181GlnThr: 2.181 ± 0.22
1.977GlnVal: 1.977 ± 0.205
0.53GlnTrp: 0.53 ± 0.115
1.141GlnTyr: 1.141 ± 0.19
0.0GlnXaa: 0.0 ± 0.0
Arg
3.159ArgAla: 3.159 ± 0.234
0.387ArgCys: 0.387 ± 0.088
3.363ArgAsp: 3.363 ± 0.253
3.017ArgGlu: 3.017 ± 0.297
3.098ArgPhe: 3.098 ± 0.268
3.139ArgGly: 3.139 ± 0.25
1.019ArgHis: 1.019 ± 0.155
3.587ArgIle: 3.587 ± 0.297
3.302ArgLys: 3.302 ± 0.298
4.219ArgLeu: 4.219 ± 0.276
1.243ArgMet: 1.243 ± 0.14
2.854ArgAsn: 2.854 ± 0.231
1.631ArgPro: 1.631 ± 0.218
1.753ArgGln: 1.753 ± 0.184
2.813ArgArg: 2.813 ± 0.281
2.894ArgSer: 2.894 ± 0.269
2.609ArgThr: 2.609 ± 0.221
4.464ArgVal: 4.464 ± 0.292
0.448ArgTrp: 0.448 ± 0.097
2.324ArgTyr: 2.324 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
3.873SerAla: 3.873 ± 0.397
0.55SerCys: 0.55 ± 0.098
4.403SerAsp: 4.403 ± 0.299
3.975SerGlu: 3.975 ± 0.281
3.547SerPhe: 3.547 ± 0.239
4.912SerGly: 4.912 ± 0.461
1.284SerHis: 1.284 ± 0.178
4.933SerIle: 4.933 ± 0.427
4.505SerLys: 4.505 ± 0.271
5.116SerLeu: 5.116 ± 0.302
1.549SerMet: 1.549 ± 0.175
3.649SerAsn: 3.649 ± 0.266
1.977SerPro: 1.977 ± 0.191
1.794SerGln: 1.794 ± 0.205
3.241SerArg: 3.241 ± 0.26
5.096SerSer: 5.096 ± 0.425
3.995SerThr: 3.995 ± 0.353
4.015SerVal: 4.015 ± 0.332
0.917SerTrp: 0.917 ± 0.123
2.792SerTyr: 2.792 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
4.178ThrAla: 4.178 ± 0.329
0.51ThrCys: 0.51 ± 0.107
4.26ThrAsp: 4.26 ± 0.367
3.608ThrGlu: 3.608 ± 0.257
3.098ThrPhe: 3.098 ± 0.231
4.566ThrGly: 4.566 ± 0.417
1.162ThrHis: 1.162 ± 0.135
4.138ThrIle: 4.138 ± 0.301
4.219ThrLys: 4.219 ± 0.284
4.871ThrLeu: 4.871 ± 0.39
1.121ThrMet: 1.121 ± 0.166
2.976ThrAsn: 2.976 ± 0.305
2.65ThrPro: 2.65 ± 0.246
1.569ThrGln: 1.569 ± 0.164
3.017ThrArg: 3.017 ± 0.242
4.708ThrSer: 4.708 ± 0.463
3.363ThrThr: 3.363 ± 0.439
4.301ThrVal: 4.301 ± 0.333
0.611ThrTrp: 0.611 ± 0.098
2.629ThrTyr: 2.629 ± 0.227
0.0ThrXaa: 0.0 ± 0.0
Val
4.464ValAla: 4.464 ± 0.353
0.591ValCys: 0.591 ± 0.106
5.157ValAsp: 5.157 ± 0.36
5.381ValGlu: 5.381 ± 0.359
3.343ValPhe: 3.343 ± 0.259
3.975ValGly: 3.975 ± 0.306
1.345ValHis: 1.345 ± 0.144
4.321ValIle: 4.321 ± 0.325
5.157ValLys: 5.157 ± 0.351
5.157ValLeu: 5.157 ± 0.282
1.671ValMet: 1.671 ± 0.195
3.363ValAsn: 3.363 ± 0.327
2.65ValPro: 2.65 ± 0.272
2.385ValGln: 2.385 ± 0.224
3.832ValArg: 3.832 ± 0.283
4.525ValSer: 4.525 ± 0.353
4.953ValThr: 4.953 ± 0.351
4.79ValVal: 4.79 ± 0.329
0.836ValTrp: 0.836 ± 0.135
2.385ValTyr: 2.385 ± 0.221
0.0ValXaa: 0.0 ± 0.0
Trp
0.489TrpAla: 0.489 ± 0.092
0.224TrpCys: 0.224 ± 0.077
0.836TrpAsp: 0.836 ± 0.148
0.693TrpGlu: 0.693 ± 0.116
0.775TrpPhe: 0.775 ± 0.13
0.347TrpGly: 0.347 ± 0.091
0.428TrpHis: 0.428 ± 0.101
1.04TrpIle: 1.04 ± 0.145
1.019TrpLys: 1.019 ± 0.124
1.08TrpLeu: 1.08 ± 0.146
0.387TrpMet: 0.387 ± 0.082
0.836TrpAsn: 0.836 ± 0.151
0.367TrpPro: 0.367 ± 0.097
0.367TrpGln: 0.367 ± 0.09
0.591TrpArg: 0.591 ± 0.111
0.754TrpSer: 0.754 ± 0.118
0.978TrpThr: 0.978 ± 0.134
0.856TrpVal: 0.856 ± 0.129
0.265TrpTrp: 0.265 ± 0.076
0.428TrpTyr: 0.428 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.192
0.387TyrCys: 0.387 ± 0.08
3.18TyrAsp: 3.18 ± 0.245
2.12TyrGlu: 2.12 ± 0.206
1.834TyrPhe: 1.834 ± 0.196
2.507TyrGly: 2.507 ± 0.236
0.713TyrHis: 0.713 ± 0.112
2.65TyrIle: 2.65 ± 0.289
2.079TyrLys: 2.079 ± 0.195
3.017TyrLeu: 3.017 ± 0.26
0.876TyrMet: 0.876 ± 0.148
1.773TyrAsn: 1.773 ± 0.177
1.447TyrPro: 1.447 ± 0.181
1.468TyrGln: 1.468 ± 0.186
2.222TyrArg: 2.222 ± 0.214
2.242TyrSer: 2.242 ± 0.234
2.344TyrThr: 2.344 ± 0.249
3.18TyrVal: 3.18 ± 0.267
0.55TyrTrp: 0.55 ± 0.097
1.692TyrTyr: 1.692 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 261 proteins (49062 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski