Sphingomonas sp. HDW15C

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7ZLN4|A0A6G7ZLN4_9SPHN RNA polymerase sigma factor OS=Sphingomonas sp. HDW15C OX=2714944 GN=G7078_02855 PE=3 SV=1
MM1 pKa = 7.81VDD3 pKa = 3.21IPGDD7 pKa = 3.6TTTTSTISVGSMVDD21 pKa = 3.38NAIEE25 pKa = 4.19TVGDD29 pKa = 3.95HH30 pKa = 6.99DD31 pKa = 4.31WYY33 pKa = 11.04AVTLTAGQKK42 pKa = 8.01VTIALNIITLEE53 pKa = 4.09DD54 pKa = 4.07PYY56 pKa = 11.56LYY58 pKa = 10.87LRR60 pKa = 11.84DD61 pKa = 4.08SNGSLIAEE69 pKa = 4.4NDD71 pKa = 3.47DD72 pKa = 3.84GGGGRR77 pKa = 11.84GSRR80 pKa = 11.84LVFTAPTDD88 pKa = 3.31GTYY91 pKa = 10.85YY92 pKa = 10.24IDD94 pKa = 3.57VAAWAPTEE102 pKa = 4.06VVPGYY107 pKa = 8.99TGTGAYY113 pKa = 9.34RR114 pKa = 11.84LSVSDD119 pKa = 3.72YY120 pKa = 9.02VAPSEE125 pKa = 4.2GTLDD129 pKa = 4.42DD130 pKa = 5.06FADD133 pKa = 3.66QMTHH137 pKa = 6.01GFFDD141 pKa = 3.86GDD143 pKa = 3.59YY144 pKa = 10.03HH145 pKa = 7.28HH146 pKa = 7.35FNVTQGGSLTVNFQGLTDD164 pKa = 4.17AGRR167 pKa = 11.84TVALQALQQWTDD179 pKa = 3.15IIGVNFVEE187 pKa = 4.72TNGAAQITFDD197 pKa = 5.75DD198 pKa = 4.67LDD200 pKa = 3.63QGTGAFADD208 pKa = 4.13TVSSNHH214 pKa = 5.36ITSSAIVNVALYY226 pKa = 10.29RR227 pKa = 11.84LNLHH231 pKa = 6.45TYY233 pKa = 6.25MHH235 pKa = 7.43EE236 pKa = 3.98IGHH239 pKa = 6.67ALGIGHH245 pKa = 7.1TSNSNAGTAGAIYY258 pKa = 10.09PNDD261 pKa = 4.07ALWSNDD267 pKa = 2.34GSAISIMSYY276 pKa = 10.72FDD278 pKa = 3.3NGEE281 pKa = 3.75NGYY284 pKa = 10.0YY285 pKa = 10.0SSRR288 pKa = 11.84GFSNLPVVTPQVADD302 pKa = 4.57IIAMGNLYY310 pKa = 10.61GLSTTTRR317 pKa = 11.84TGNTTYY323 pKa = 11.17GFNNTSGRR331 pKa = 11.84AAFDD335 pKa = 3.54AALHH339 pKa = 6.09PNYY342 pKa = 10.49SYY344 pKa = 11.07TIFDD348 pKa = 3.57NGGVDD353 pKa = 3.31TLDD356 pKa = 3.28YY357 pKa = 11.41SGFISNQLIDD367 pKa = 4.73LNPEE371 pKa = 3.71AFSNIGAFVGNVVIARR387 pKa = 11.84GTVIEE392 pKa = 4.05NAIGGSGNDD401 pKa = 3.41TLVGNGAANVLTGNSGIDD419 pKa = 3.34TLYY422 pKa = 11.17GADD425 pKa = 4.46GNDD428 pKa = 3.49TLTGGLGADD437 pKa = 3.56VLVGGTGGDD446 pKa = 3.87FFRR449 pKa = 11.84DD450 pKa = 3.28TAAGLNGDD458 pKa = 4.28TIVDD462 pKa = 4.45FSAQDD467 pKa = 3.42RR468 pKa = 11.84IVITNVGLASFSYY481 pKa = 10.68SLVGNTLTYY490 pKa = 10.26TGGSLTFSNALAGHH504 pKa = 6.67LFVRR508 pKa = 11.84AADD511 pKa = 3.79GGGVEE516 pKa = 4.92LQMADD521 pKa = 3.0RR522 pKa = 11.84TGFGLTDD529 pKa = 3.79FNGDD533 pKa = 4.13GIADD537 pKa = 3.88VLWRR541 pKa = 11.84SDD543 pKa = 3.23TGVITTWLGQADD555 pKa = 4.13GTFIDD560 pKa = 4.07NSANTGQAIPLDD572 pKa = 3.92WNIVGTGDD580 pKa = 3.67YY581 pKa = 11.04NGDD584 pKa = 3.62GLGDD588 pKa = 3.57IMWRR592 pKa = 11.84SDD594 pKa = 3.85SGVMTQWLGQLDD606 pKa = 3.93GTFRR610 pKa = 11.84DD611 pKa = 3.9NFAKK615 pKa = 9.74TGQVIPLDD623 pKa = 3.61WNVIGTGDD631 pKa = 3.78FNGDD635 pKa = 3.42GLGDD639 pKa = 4.64LIWRR643 pKa = 11.84SDD645 pKa = 3.26AGVITEE651 pKa = 4.32WLGNLDD657 pKa = 4.03GSFRR661 pKa = 11.84DD662 pKa = 3.79NFAGTGQHH670 pKa = 6.8IPLNWTIDD678 pKa = 3.28GMGDD682 pKa = 3.48FNGDD686 pKa = 3.51GLEE689 pKa = 3.97DD690 pKa = 4.84LIWRR694 pKa = 11.84SDD696 pKa = 3.19AGVITEE702 pKa = 4.5WLGQNDD708 pKa = 4.51GSFKK712 pKa = 11.28DD713 pKa = 3.74NFAGTGQTIPTNWQIVGVGDD733 pKa = 4.48FNADD737 pKa = 3.17GYY739 pKa = 11.89ADD741 pKa = 4.33ILWQSTAGTLTNWLGNPDD759 pKa = 4.32GSFTDD764 pKa = 3.56NFVNTGQVIPQGWEE778 pKa = 3.63LVGVGDD784 pKa = 4.91LNGDD788 pKa = 3.51GRR790 pKa = 11.84DD791 pKa = 3.85DD792 pKa = 4.25ILWQTGPDD800 pKa = 3.65SVEE803 pKa = 3.58TWFGQDD809 pKa = 2.52NGGFTGGSSGTLAAAHH825 pKa = 6.74AATAGTNGDD834 pKa = 4.03LFYY837 pKa = 11.18LGG839 pKa = 4.25

Molecular weight: 88.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7ZQW8|A0A6G7ZQW8_9SPHN Probable multidrug resistance protein NorM OS=Sphingomonas sp. HDW15C OX=2714944 GN=G7078_04370 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLNARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.44LSAA44 pKa = 3.94

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2087

0

2087

640540

41

1791

306.9

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.1 ± 0.083

0.761 ± 0.016

5.913 ± 0.045

5.748 ± 0.057

3.541 ± 0.035

8.84 ± 0.052

1.836 ± 0.024

4.923 ± 0.036

3.261 ± 0.044

10.0 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.023

2.495 ± 0.029

5.334 ± 0.042

3.285 ± 0.03

7.537 ± 0.057

5.391 ± 0.036

4.944 ± 0.038

7.352 ± 0.044

1.372 ± 0.025

2.096 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski